Seinonella peptonophila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Seinonella

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4TLC5|A0A1M4TLC5_9BACL 2-oxoisovalerate dehydrogenase subunit alpha OS=Seinonella peptonophila OX=112248 GN=SAMN05444392_101518 PE=3 SV=1
MM1 pKa = 7.27FLVTEE6 pKa = 5.03PIMRR10 pKa = 11.84TWVDD14 pKa = 2.89KK15 pKa = 9.33DD16 pKa = 3.37TCIACGACGATAPDD30 pKa = 3.76VYY32 pKa = 10.95DD33 pKa = 3.8YY34 pKa = 11.69DD35 pKa = 4.14EE36 pKa = 6.24DD37 pKa = 5.94GIAYY41 pKa = 10.06VILDD45 pKa = 4.85DD46 pKa = 3.96NTATADD52 pKa = 3.66VPEE55 pKa = 4.67EE56 pKa = 3.81FHH58 pKa = 7.85DD59 pKa = 4.47DD60 pKa = 3.38VRR62 pKa = 11.84DD63 pKa = 3.85AQEE66 pKa = 4.25GCPTDD71 pKa = 3.78SIKK74 pKa = 10.82VEE76 pKa = 4.13EE77 pKa = 4.22

Molecular weight: 8.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4WCK5|A0A1M4WCK5_9BACL Phosphatidylserine decarboxylase proenzyme OS=Seinonella peptonophila OX=112248 GN=psd PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84LITTLLIGGAALMAGRR18 pKa = 11.84AMMRR22 pKa = 11.84RR23 pKa = 11.84NRR25 pKa = 11.84SDD27 pKa = 3.09TLRR30 pKa = 11.84LLRR33 pKa = 11.84RR34 pKa = 11.84YY35 pKa = 10.51GKK37 pKa = 8.79MAGKK41 pKa = 10.77AMMGRR46 pKa = 11.84RR47 pKa = 11.84MIRR50 pKa = 11.84RR51 pKa = 11.84LWMAA55 pKa = 4.38

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3703

0

3703

1053506

29

3784

284.5

32.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.634 ± 0.037

0.852 ± 0.012

4.783 ± 0.03

6.782 ± 0.041

4.379 ± 0.033

6.48 ± 0.034

2.495 ± 0.022

7.737 ± 0.038

6.218 ± 0.047

10.453 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.018

3.804 ± 0.03

3.962 ± 0.027

5.251 ± 0.042

5.0 ± 0.033

6.066 ± 0.03

5.244 ± 0.029

6.539 ± 0.03

1.308 ± 0.017

3.403 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski