Wenling hepe-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KKF8|A0A1L3KKF8_9VIRU Uncharacterized protein OS=Wenling hepe-like virus 4 OX=1923496 PE=4 SV=1
MM1 pKa = 7.38SSISSFEE8 pKa = 4.32EE9 pKa = 3.61IEE11 pKa = 4.26MISDD15 pKa = 4.17FKK17 pKa = 11.51KK18 pKa = 10.12EE19 pKa = 4.09LEE21 pKa = 4.25SFALSPKK28 pKa = 10.32AIYY31 pKa = 9.14QQYY34 pKa = 10.11CFPCMHH40 pKa = 7.51DD41 pKa = 4.18EE42 pKa = 4.15YY43 pKa = 10.76TLNDD47 pKa = 4.01SIYY50 pKa = 10.8LRR52 pKa = 11.84VFDD55 pKa = 4.25FTFVLPATNTYY66 pKa = 11.12DD67 pKa = 4.24DD68 pKa = 4.54LWNIAADD75 pKa = 3.29QFGFLRR81 pKa = 11.84HH82 pKa = 6.26YY83 pKa = 11.1INDD86 pKa = 3.77HH87 pKa = 5.78NFQKK91 pKa = 10.86YY92 pKa = 10.13LEE94 pKa = 4.37FFSRR98 pKa = 11.84FCPNFKK104 pKa = 10.61FMLDD108 pKa = 3.58FGDD111 pKa = 4.03GEE113 pKa = 4.59VIVRR117 pKa = 11.84IVHH120 pKa = 6.08NSASRR125 pKa = 11.84VFEE128 pKa = 3.86EE129 pKa = 4.44HH130 pKa = 6.81SYY132 pKa = 9.12EE133 pKa = 4.14TFSTSGYY140 pKa = 10.36SIGEE144 pKa = 4.05AVKK147 pKa = 9.48VTLQAFFNSEE157 pKa = 4.04TFRR160 pKa = 11.84LISKK164 pKa = 10.09SEE166 pKa = 3.67II167 pKa = 3.47

Molecular weight: 19.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KK85|A0A1L3KK85_9VIRU Putative structural protein OS=Wenling hepe-like virus 4 OX=1923496 PE=4 SV=1
MM1 pKa = 7.62ASFFYY6 pKa = 10.92VCLFAFIISAEE17 pKa = 4.11PKK19 pKa = 9.57KK20 pKa = 10.55NLRR23 pKa = 11.84PTVLNVLLRR32 pKa = 11.84IEE34 pKa = 4.29KK35 pKa = 10.52VLIGLDD41 pKa = 3.58SEE43 pKa = 4.51IAALSASTVASNSLLMSVKK62 pKa = 10.25NYY64 pKa = 9.83QKK66 pKa = 10.78NIEE69 pKa = 4.27RR70 pKa = 11.84NTNKK74 pKa = 9.96QYY76 pKa = 11.58SLTQNWNSVINHH88 pKa = 5.1VDD90 pKa = 3.25RR91 pKa = 11.84FVKK94 pKa = 10.62LDD96 pKa = 3.59GKK98 pKa = 10.34HH99 pKa = 6.85DD100 pKa = 3.67IVKK103 pKa = 10.61AGAPGTAMKK112 pKa = 10.95GEE114 pKa = 4.11LKK116 pKa = 10.68GRR118 pKa = 11.84GWLTCPAFKK127 pKa = 10.7GSTQLCKK134 pKa = 10.69YY135 pKa = 7.75VFPHH139 pKa = 6.44ARR141 pKa = 11.84YY142 pKa = 9.15DD143 pKa = 3.5VNRR146 pKa = 11.84AGEE149 pKa = 4.13ASRR152 pKa = 11.84FTPDD156 pKa = 2.56FWGKK160 pKa = 10.55KK161 pKa = 6.5YY162 pKa = 11.03

Molecular weight: 18.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3231

162

1738

538.5

60.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.252 ± 0.678

2.631 ± 0.218

6.097 ± 0.474

5.633 ± 0.968

6.035 ± 0.841

5.323 ± 0.33

2.105 ± 0.501

5.602 ± 0.354

5.911 ± 0.899

8.511 ± 0.451

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.919 ± 0.208

4.952 ± 0.208

3.838 ± 0.525

3.59 ± 0.394

4.673 ± 0.576

8.48 ± 1.624

5.509 ± 0.865

7.954 ± 0.647

0.836 ± 0.203

4.147 ± 0.581
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski