Rift valley fever virus (strain ZH-548 M12) (RVFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Rift Valley fever phlebovirus

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P21700|NCAP_RVFVZ Nucleoprotein OS=Rift valley fever virus (strain ZH-548 M12) OX=11589 GN=N PE=1 SV=1
MM1 pKa = 8.36DD2 pKa = 4.9YY3 pKa = 10.96FPVISVDD10 pKa = 3.68LQSGRR15 pKa = 11.84RR16 pKa = 11.84VVSVEE21 pKa = 3.54YY22 pKa = 10.58FRR24 pKa = 11.84GDD26 pKa = 3.39GPPRR30 pKa = 11.84IPYY33 pKa = 10.8SMVGPCCVFLMHH45 pKa = 6.78HH46 pKa = 6.51RR47 pKa = 11.84PSHH50 pKa = 4.79EE51 pKa = 3.6VRR53 pKa = 11.84LRR55 pKa = 11.84FSDD58 pKa = 4.43FYY60 pKa = 11.69NVGEE64 pKa = 3.95FPYY67 pKa = 10.48RR68 pKa = 11.84VGLGDD73 pKa = 3.64FASNVAPPPAKK84 pKa = 10.09PFQRR88 pKa = 11.84LIDD91 pKa = 4.95LIGHH95 pKa = 5.42MTLSDD100 pKa = 3.52FTRR103 pKa = 11.84FPNLKK108 pKa = 9.9EE109 pKa = 4.39AISWPLGEE117 pKa = 4.82PSLAFFDD124 pKa = 4.54LSSTRR129 pKa = 11.84VHH131 pKa = 6.71RR132 pKa = 11.84NDD134 pKa = 5.09DD135 pKa = 3.07IRR137 pKa = 11.84RR138 pKa = 11.84DD139 pKa = 3.87QIATLAMRR147 pKa = 11.84SCKK150 pKa = 8.73ITNDD154 pKa = 3.67LEE156 pKa = 4.43DD157 pKa = 4.02SFVGLHH163 pKa = 6.38RR164 pKa = 11.84MIATEE169 pKa = 4.63AILRR173 pKa = 11.84GIDD176 pKa = 3.45LCLLPGFDD184 pKa = 4.68LMYY187 pKa = 10.65EE188 pKa = 4.3VAHH191 pKa = 5.14VQCVRR196 pKa = 11.84LLQAAKK202 pKa = 10.31EE203 pKa = 4.41DD204 pKa = 3.32ISNAVVPNSALIVLMEE220 pKa = 4.43EE221 pKa = 4.29SLMLRR226 pKa = 11.84SSLPSMMGRR235 pKa = 11.84NNWIPVIPPIPDD247 pKa = 2.86VEE249 pKa = 4.31MEE251 pKa = 4.3SEE253 pKa = 4.35EE254 pKa = 4.48EE255 pKa = 3.98SDD257 pKa = 3.98DD258 pKa = 4.23DD259 pKa = 5.24GFVEE263 pKa = 4.86VDD265 pKa = 2.97

Molecular weight: 29.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P21401-5|GP-5_RVFVZ Isoform of P21401 Isoform NSm' protein of Envelopment polyprotein OS=Rift valley fever virus (strain ZH-548 M12) OX=11589 GN=GP PE=1 SV=1
MM1 pKa = 7.79PEE3 pKa = 4.33EE4 pKa = 4.46LSCSISGIRR13 pKa = 11.84EE14 pKa = 4.08VKK16 pKa = 9.61TSSQEE21 pKa = 3.56LYY23 pKa = 10.04RR24 pKa = 11.84ALKK27 pKa = 10.43AIIAADD33 pKa = 3.58GLNNITCHH41 pKa = 6.42GKK43 pKa = 10.26DD44 pKa = 3.6PEE46 pKa = 4.67DD47 pKa = 4.53KK48 pKa = 10.41ISLIKK53 pKa = 10.62GPPHH57 pKa = 6.89KK58 pKa = 10.26KK59 pKa = 9.01RR60 pKa = 11.84VGIVRR65 pKa = 11.84CEE67 pKa = 3.61RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84DD71 pKa = 3.3AKK73 pKa = 10.8QIGRR77 pKa = 11.84KK78 pKa = 7.15TMAGIAMTVLPALAVFALAPVVFAA102 pKa = 5.8

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

2

6

4073

102

2149

678.8

75.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.85 ± 1.237

2.946 ± 0.868

5.009 ± 0.404

6.359 ± 0.326

4.591 ± 0.493

6.31 ± 0.554

2.431 ± 0.15

6.187 ± 0.565

6.113 ± 0.589

9.305 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.192 ± 0.533

3.585 ± 0.246

4.493 ± 0.588

3.094 ± 0.281

5.573 ± 0.773

8.618 ± 0.738

4.739 ± 0.519

6.752 ± 0.297

1.154 ± 0.13

2.701 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski