Salmonella phage phSE-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Tlsvirus; Salmonella virus phSE2

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160CA49|A0A160CA49_9CAUD Uncharacterized protein OS=Salmonella phage phSE-2 OX=1837218 PE=4 SV=1
MM1 pKa = 7.39AVYY4 pKa = 8.78TGKK7 pKa = 9.09MFEE10 pKa = 4.59SEE12 pKa = 3.94NPIYY16 pKa = 10.75GGTYY20 pKa = 9.93EE21 pKa = 5.7LIIDD25 pKa = 4.03EE26 pKa = 4.9DD27 pKa = 4.29RR28 pKa = 11.84VILADD33 pKa = 5.19DD34 pKa = 4.83DD35 pKa = 3.83NQEE38 pKa = 4.18YY39 pKa = 10.68YY40 pKa = 10.41EE41 pKa = 4.27VEE43 pKa = 3.68ADD45 pKa = 3.2KK46 pKa = 10.86WDD48 pKa = 3.81NDD50 pKa = 3.66VMFIVDD56 pKa = 3.3NSRR59 pKa = 11.84IKK61 pKa = 10.27FYY63 pKa = 10.94AVEE66 pKa = 4.06EE67 pKa = 4.45EE68 pKa = 4.37EE69 pKa = 4.19

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160CAA1|A0A160CAA1_9CAUD Uncharacterized protein OS=Salmonella phage phSE-2 OX=1837218 PE=4 SV=1
MM1 pKa = 7.77TYY3 pKa = 10.56FKK5 pKa = 10.66NIKK8 pKa = 8.02TGRR11 pKa = 11.84IYY13 pKa = 10.67RR14 pKa = 11.84VNIRR18 pKa = 11.84HH19 pKa = 6.0KK20 pKa = 10.62NALIMSKK27 pKa = 10.07GGCWVRR33 pKa = 11.84SARR36 pKa = 11.84YY37 pKa = 9.47RR38 pKa = 11.84NADD41 pKa = 3.14LTGYY45 pKa = 9.67PFIAVNSTNCC55 pKa = 3.54

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

14818

38

1258

178.5

20.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.496 ± 0.42

1.329 ± 0.185

6.249 ± 0.203

7.234 ± 0.262

4.09 ± 0.181

7.126 ± 0.276

1.809 ± 0.174

6.58 ± 0.215

7.376 ± 0.402

6.843 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.111 ± 0.166

4.92 ± 0.269

3.145 ± 0.199

3.853 ± 0.269

4.926 ± 0.202

5.52 ± 0.241

5.352 ± 0.268

6.85 ± 0.306

1.518 ± 0.114

3.671 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski