Klebsiella phage K64-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Alcyoneusvirus; Klebsiella virus K64-1

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8J9R3|A0A0A8J9R3_9CAUD Uncharacterized protein OS=Klebsiella phage K64-1 OX=1439894 PE=4 SV=1
MM1 pKa = 7.59TISFNNGSNCSCNNSGIKK19 pKa = 9.92SMKK22 pKa = 9.81ISTDD26 pKa = 2.29GHH28 pKa = 6.85LIVTYY33 pKa = 10.87ADD35 pKa = 3.38NTIEE39 pKa = 4.68DD40 pKa = 3.74LGLVVGHH47 pKa = 7.25DD48 pKa = 4.03GSDD51 pKa = 3.22GSNFYY56 pKa = 10.49PNEE59 pKa = 3.93IGFEE63 pKa = 4.06IPDD66 pKa = 3.55SDD68 pKa = 4.22FNTDD72 pKa = 3.26KK73 pKa = 11.11TIGWSYY79 pKa = 11.41LSLANSTSVLYY90 pKa = 10.26FKK92 pKa = 10.26TSNPGEE98 pKa = 4.14SSTWNSVEE106 pKa = 4.11FGKK109 pKa = 11.04GEE111 pKa = 3.81QGEE114 pKa = 4.35QGKK117 pKa = 8.62PFHH120 pKa = 7.12IDD122 pKa = 2.48SSGTVFPTSGLFDD135 pKa = 4.66GYY137 pKa = 9.47TFYY140 pKa = 10.67NTNDD144 pKa = 3.27GKK146 pKa = 11.35LYY148 pKa = 10.32FYY150 pKa = 10.89DD151 pKa = 4.74SSTQTWSSGISFRR164 pKa = 11.84GEE166 pKa = 3.43VGPRR170 pKa = 11.84GRR172 pKa = 11.84FHH174 pKa = 7.4IDD176 pKa = 2.86STGSTFPDD184 pKa = 3.07ITNLPEE190 pKa = 5.22DD191 pKa = 3.73YY192 pKa = 10.73CFYY195 pKa = 10.09NTSDD199 pKa = 3.01GKK201 pKa = 10.89LYY203 pKa = 10.98YY204 pKa = 10.4VDD206 pKa = 3.75IVEE209 pKa = 4.51NTDD212 pKa = 3.47PVQKK216 pKa = 9.97EE217 pKa = 3.78WSTGIEE223 pKa = 3.97FRR225 pKa = 11.84GKK227 pKa = 10.45EE228 pKa = 3.97GLQGEE233 pKa = 4.62KK234 pKa = 10.38GDD236 pKa = 4.66KK237 pKa = 10.77GDD239 pKa = 3.64AGKK242 pKa = 10.51DD243 pKa = 2.75IVVIVNTIDD252 pKa = 3.09HH253 pKa = 6.87SYY255 pKa = 9.64TNTVLVIGTCPAGYY269 pKa = 9.92LVTNIEE275 pKa = 4.34VNVTQGYY282 pKa = 7.33EE283 pKa = 3.98PPVTDD288 pKa = 3.11MKK290 pKa = 10.91IRR292 pKa = 11.84FSGEE296 pKa = 3.54ANEE299 pKa = 5.45DD300 pKa = 3.03IGGTVIAGYY309 pKa = 10.55DD310 pKa = 3.48YY311 pKa = 11.4FDD313 pKa = 4.41ISMQSRR319 pKa = 11.84YY320 pKa = 10.25VINEE324 pKa = 3.88TNHH327 pKa = 5.52IVSDD331 pKa = 3.42KK332 pKa = 11.52DD333 pKa = 3.84EE334 pKa = 4.99IISCIFNEE342 pKa = 4.26SVNLSSYY349 pKa = 11.71GNMTIICTLSWQAPTLPISDD369 pKa = 4.65NII371 pKa = 3.84

Molecular weight: 40.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8J9Y6|A0A0A8J9Y6_9CAUD Putative Hef-like homing endonuclease OS=Klebsiella phage K64-1 OX=1439894 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.25KK3 pKa = 10.15YY4 pKa = 10.74LKK6 pKa = 10.86NLDD9 pKa = 3.98TEE11 pKa = 4.59TEE13 pKa = 3.95NLFNEE18 pKa = 3.99IRR20 pKa = 11.84SLLSNKK26 pKa = 8.93NSKK29 pKa = 8.95SWHH32 pKa = 5.03TSINASGRR40 pKa = 11.84EE41 pKa = 3.94HH42 pKa = 7.07LKK44 pKa = 10.36NAVLRR49 pKa = 11.84VYY51 pKa = 10.33PSSDD55 pKa = 3.23FLLSEE60 pKa = 4.04ALKK63 pKa = 10.9LIINQEE69 pKa = 3.99EE70 pKa = 4.19PPYY73 pKa = 10.2AVCGRR78 pKa = 11.84KK79 pKa = 9.11CKK81 pKa = 10.48YY82 pKa = 10.66FNGQYY87 pKa = 10.87DD88 pKa = 4.5RR89 pKa = 11.84YY90 pKa = 9.78CSLGVKK96 pKa = 9.29PNKK99 pKa = 8.78NTYY102 pKa = 9.35CEE104 pKa = 4.14KK105 pKa = 10.73CNKK108 pKa = 8.89HH109 pKa = 4.65WRR111 pKa = 11.84EE112 pKa = 4.04KK113 pKa = 10.46KK114 pKa = 10.36NKK116 pKa = 7.45STKK119 pKa = 9.69EE120 pKa = 3.77NCLNQYY126 pKa = 9.48NVEE129 pKa = 4.47HH130 pKa = 7.0PFQRR134 pKa = 11.84NDD136 pKa = 2.87VKK138 pKa = 10.57TKK140 pKa = 8.68VHH142 pKa = 4.8EE143 pKa = 4.32TKK145 pKa = 9.83IRR147 pKa = 11.84KK148 pKa = 9.39YY149 pKa = 7.98GTNYY153 pKa = 10.67NKK155 pKa = 10.22IISNKK160 pKa = 9.97SFSTYY165 pKa = 9.74YY166 pKa = 10.86NKK168 pKa = 10.02TGYY171 pKa = 9.91SCPLSNPDD179 pKa = 2.88IQQKK183 pKa = 10.04IKK185 pKa = 8.99EE186 pKa = 4.59TNIKK190 pKa = 10.35NYY192 pKa = 10.27GYY194 pKa = 9.08EE195 pKa = 4.26NPGSSPLIVQKK206 pKa = 10.79RR207 pKa = 11.84KK208 pKa = 8.13NTQKK212 pKa = 10.83KK213 pKa = 10.15LYY215 pKa = 8.75GHH217 pKa = 6.02EE218 pKa = 4.51HH219 pKa = 6.04SMQNGDD225 pKa = 3.27IKK227 pKa = 11.18LKK229 pKa = 10.93VLIKK233 pKa = 10.63SATQRR238 pKa = 11.84MDD240 pKa = 3.9SINSKK245 pKa = 11.21LEE247 pKa = 3.69FTNFIPLFDD256 pKa = 4.01ISDD259 pKa = 4.89LIGLDD264 pKa = 3.84TYY266 pKa = 11.99NKK268 pKa = 8.92MPYY271 pKa = 8.25WCNTHH276 pKa = 6.27GLFYY280 pKa = 10.85SCSNYY285 pKa = 10.42KK286 pKa = 10.06IICNEE291 pKa = 3.98CTPVHH296 pKa = 6.97ISTQHH301 pKa = 6.46KK302 pKa = 9.81EE303 pKa = 3.54ILNFIEE309 pKa = 4.53NLGIEE314 pKa = 4.71CVTNTRR320 pKa = 11.84SVLKK324 pKa = 9.85STMEE328 pKa = 4.0LDD330 pKa = 4.09IFIPDD335 pKa = 3.7LNVAIEE341 pKa = 4.0INGMYY346 pKa = 8.8WHH348 pKa = 5.75STEE351 pKa = 4.03YY352 pKa = 10.67KK353 pKa = 9.84EE354 pKa = 5.69RR355 pKa = 11.84LYY357 pKa = 10.72HH358 pKa = 4.69QNKK361 pKa = 8.19MLEE364 pKa = 4.37CKK366 pKa = 9.68EE367 pKa = 4.17HH368 pKa = 6.84GIHH371 pKa = 7.97LIQIWEE377 pKa = 4.45DD378 pKa = 3.04EE379 pKa = 4.5WNTKK383 pKa = 8.96QDD385 pKa = 3.58LVKK388 pKa = 10.4FRR390 pKa = 11.84LMNVLKK396 pKa = 10.5CSSTIYY402 pKa = 10.56ARR404 pKa = 11.84KK405 pKa = 9.92CKK407 pKa = 10.08IKK409 pKa = 10.33EE410 pKa = 4.03INKK413 pKa = 9.63KK414 pKa = 10.06EE415 pKa = 3.85CDD417 pKa = 3.44NFINTYY423 pKa = 9.61HH424 pKa = 6.38IQGSSSSSIRR434 pKa = 11.84IGLYY438 pKa = 10.01YY439 pKa = 10.46EE440 pKa = 4.22NEE442 pKa = 4.29LYY444 pKa = 11.04AVMTFGKK451 pKa = 10.32SRR453 pKa = 11.84FDD455 pKa = 3.1KK456 pKa = 9.88TVQYY460 pKa = 11.04EE461 pKa = 4.25LIRR464 pKa = 11.84YY465 pKa = 7.4CSKK468 pKa = 9.64STVVGGASKK477 pKa = 10.58LLKK480 pKa = 10.65YY481 pKa = 10.24FEE483 pKa = 4.73KK484 pKa = 10.39KK485 pKa = 10.02YY486 pKa = 10.88KK487 pKa = 9.42PVSLISYY494 pKa = 10.9ADD496 pKa = 3.8ACWSTGDD503 pKa = 4.66LYY505 pKa = 10.78IKK507 pKa = 10.77LGFTYY512 pKa = 10.7NGLTEE517 pKa = 4.5PGYY520 pKa = 10.44FYY522 pKa = 11.19YY523 pKa = 10.56KK524 pKa = 10.41SSTGRR529 pKa = 11.84ISRR532 pKa = 11.84HH533 pKa = 3.54QCQKK537 pKa = 11.01KK538 pKa = 9.71KK539 pKa = 10.64LVNQGYY545 pKa = 10.15DD546 pKa = 3.43EE547 pKa = 4.64NLSEE551 pKa = 4.16YY552 pKa = 10.25QICTTVLKK560 pKa = 10.45YY561 pKa = 11.18NKK563 pKa = 9.77IFDD566 pKa = 4.04CGNFKK571 pKa = 9.11FTKK574 pKa = 10.39YY575 pKa = 10.77YY576 pKa = 9.77II577 pKa = 3.9

Molecular weight: 67.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

39404

343

3321

615.7

69.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.548 ± 0.212

1.178 ± 0.115

6.563 ± 0.219

5.779 ± 0.274

4.281 ± 0.107

6.357 ± 0.256

1.391 ± 0.088

7.588 ± 0.181

6.644 ± 0.294

7.301 ± 0.163

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.096

7.606 ± 0.239

3.304 ± 0.11

3.672 ± 0.125

3.528 ± 0.128

8.382 ± 0.232

7.268 ± 0.266

6.228 ± 0.179

1.145 ± 0.057

5.017 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski