Rhinolophus simulator polyomavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223Z965|A0A223Z965_9POLY Minor capsid protein VP2 OS=Rhinolophus simulator polyomavirus 2 OX=2029305 GN=VP3 PE=3 SV=1
MM1 pKa = 7.32GAFLAVLAEE10 pKa = 4.05VFEE13 pKa = 4.78LSSITGLSVEE23 pKa = 4.49TFLSGEE29 pKa = 4.08AFATAEE35 pKa = 4.36LLEE38 pKa = 4.25SHH40 pKa = 7.02ISNLVVYY47 pKa = 10.33GGLTEE52 pKa = 4.68AEE54 pKa = 4.46AIAATEE60 pKa = 4.21ISAEE64 pKa = 4.19AYY66 pKa = 9.42SALTSLEE73 pKa = 3.87ATFPQAFTALATTEE87 pKa = 4.06LATTGTLTVGAAIAAALYY105 pKa = 9.09PYY107 pKa = 10.4YY108 pKa = 10.6YY109 pKa = 10.43DD110 pKa = 4.18LSVPIANLDD119 pKa = 3.27RR120 pKa = 11.84TMALQIYY127 pKa = 9.19FPDD130 pKa = 3.68YY131 pKa = 11.2DD132 pKa = 3.35IDD134 pKa = 4.28FPGVRR139 pKa = 11.84ALARR143 pKa = 11.84FLNYY147 pKa = 9.32IDD149 pKa = 5.06PYY151 pKa = 9.41TWASDD156 pKa = 3.75LYY158 pKa = 10.72HH159 pKa = 7.04SIGRR163 pKa = 11.84HH164 pKa = 3.4FWEE167 pKa = 4.23RR168 pKa = 11.84VQRR171 pKa = 11.84YY172 pKa = 6.37GQNVLQQQLEE182 pKa = 4.24QQTRR186 pKa = 11.84ALAARR191 pKa = 11.84GVQSVSEE198 pKa = 4.61LIAQYY203 pKa = 10.61FEE205 pKa = 3.75NARR208 pKa = 11.84WAITSGPQTFYY219 pKa = 11.64NSLQAYY225 pKa = 8.49YY226 pKa = 10.81SEE228 pKa = 4.89LPGVNPIQARR238 pKa = 11.84DD239 pKa = 3.32LYY241 pKa = 10.98RR242 pKa = 11.84RR243 pKa = 11.84LGEE246 pKa = 4.32NMPQRR251 pKa = 11.84YY252 pKa = 9.01GISADD257 pKa = 4.12AIADD261 pKa = 3.79QQSAEE266 pKa = 4.18YY267 pKa = 9.42VHH269 pKa = 7.2RR270 pKa = 11.84EE271 pKa = 4.05GPPGGAEE278 pKa = 4.22QRR280 pKa = 11.84TTPDD284 pKa = 2.28WMLPLILGLYY294 pKa = 10.49GEE296 pKa = 5.2LTPSWSHH303 pKa = 4.87TLEE306 pKa = 4.07EE307 pKa = 4.79LEE309 pKa = 4.68EE310 pKa = 4.49EE311 pKa = 4.01EE312 pKa = 5.96DD313 pKa = 4.05GPKK316 pKa = 10.04KK317 pKa = 10.41KK318 pKa = 10.27KK319 pKa = 10.08LRR321 pKa = 11.84RR322 pKa = 11.84DD323 pKa = 3.25TT324 pKa = 3.8

Molecular weight: 35.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223Z924|A0A223Z924_9POLY Minor capsid protein OS=Rhinolophus simulator polyomavirus 2 OX=2029305 GN=VP2 PE=3 SV=1
MM1 pKa = 7.42AHH3 pKa = 7.34LSGKK7 pKa = 10.33SGGKK11 pKa = 9.37NLIQSLIYY19 pKa = 10.21FVMKK23 pKa = 10.58QSLKK27 pKa = 10.22MILRR31 pKa = 11.84PLTKK35 pKa = 9.63EE36 pKa = 3.59RR37 pKa = 11.84QRR39 pKa = 11.84MKK41 pKa = 9.59THH43 pKa = 5.64HH44 pKa = 5.88QGPRR48 pKa = 11.84LLHH51 pKa = 5.96LRR53 pKa = 11.84RR54 pKa = 11.84KK55 pKa = 10.04KK56 pKa = 10.6KK57 pKa = 9.38ILPRR61 pKa = 11.84GTYY64 pKa = 10.5LKK66 pKa = 10.38II67 pKa = 3.83

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1776

67

657

296.0

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.025 ± 1.641

2.421 ± 0.951

4.899 ± 0.425

7.601 ± 0.683

4.673 ± 0.599

6.137 ± 0.71

2.309 ± 0.223

4.73 ± 0.288

6.025 ± 1.616

10.586 ± 0.912

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.446

3.998 ± 0.816

5.912 ± 0.762

4.955 ± 0.633

5.068 ± 0.58

5.856 ± 0.226

5.743 ± 0.675

5.068 ± 0.829

1.52 ± 0.268

4.11 ± 0.749
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski