Streptococcus phage phiNJ2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4JU73|K4JU73_9CAUD Putative recombinase OS=Streptococcus phage phiNJ2 OX=1239381 GN=phiNJ2_001 PE=4 SV=1
MM1 pKa = 8.01ADD3 pKa = 3.33GTEE6 pKa = 4.3LDD8 pKa = 5.35DD9 pKa = 3.2ITGYY13 pKa = 10.58VIPDD17 pKa = 3.91DD18 pKa = 4.02NPVYY22 pKa = 10.56EE23 pKa = 4.55IFRR26 pKa = 11.84KK27 pKa = 9.73INEE30 pKa = 3.98DD31 pKa = 3.0RR32 pKa = 11.84RR33 pKa = 11.84EE34 pKa = 4.1GNRR37 pKa = 3.31

Molecular weight: 4.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4JU44|K4JU44_9CAUD Uncharacterized protein OS=Streptococcus phage phiNJ2 OX=1239381 GN=phiNJ2_0021 PE=4 SV=1
MM1 pKa = 7.88ADD3 pKa = 3.05VKK5 pKa = 10.62VTVNLDD11 pKa = 3.14GVEE14 pKa = 4.16RR15 pKa = 11.84KK16 pKa = 9.68VSPQAMQRR24 pKa = 11.84GKK26 pKa = 10.15IATGSEE32 pKa = 3.86ALLIMDD38 pKa = 4.07SSVPLRR44 pKa = 11.84AGGGALRR51 pKa = 11.84ASGRR55 pKa = 11.84VEE57 pKa = 3.97PNGDD61 pKa = 2.9ASYY64 pKa = 8.68NTVYY68 pKa = 10.94ARR70 pKa = 11.84AQFHH74 pKa = 5.45GTNGIVVFKK83 pKa = 10.58KK84 pKa = 9.32YY85 pKa = 6.29TTSGTGKK92 pKa = 10.28RR93 pKa = 11.84WDD95 pKa = 4.07KK96 pKa = 10.35PLKK99 pKa = 10.87ANIEE103 pKa = 4.18RR104 pKa = 11.84LKK106 pKa = 10.67KK107 pKa = 10.31AAIKK111 pKa = 10.82GMGIRR116 pKa = 4.21

Molecular weight: 12.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11796

37

1269

214.5

24.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.486 ± 0.657

0.509 ± 0.096

6.629 ± 0.392

6.451 ± 0.558

3.772 ± 0.171

6.901 ± 0.494

1.441 ± 0.129

7.113 ± 0.297

7.765 ± 0.466

7.503 ± 0.378

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.442 ± 0.166

5.79 ± 0.321

2.857 ± 0.18

4.23 ± 0.291

3.84 ± 0.28

6.587 ± 0.469

6.587 ± 0.433

6.757 ± 0.319

1.272 ± 0.163

4.069 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski