Rhodococcus sp. LHW51113

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S3BNM3|A0A3S3BNM3_9NOCA DUF4233 domain-containing protein OS=Rhodococcus sp. LHW51113 OX=2487364 GN=EGT50_02690 PE=4 SV=1
MM1 pKa = 7.24FGVVSVSAMALGVAGMGAGAATADD25 pKa = 3.76TVVDD29 pKa = 3.89TVRR32 pKa = 11.84VGTAPFGVAVSPDD45 pKa = 2.97GSRR48 pKa = 11.84AYY50 pKa = 8.63VTNVVSDD57 pKa = 3.95SVSVIDD63 pKa = 3.7TAGNTVIDD71 pKa = 4.4SIGVGDD77 pKa = 4.58APAGVAVSPNGSHH90 pKa = 6.96VYY92 pKa = 8.51VTNQLEE98 pKa = 4.44DD99 pKa = 3.58TVSVIEE105 pKa = 4.19TAGNTVIDD113 pKa = 4.41TIEE116 pKa = 4.23VGDD119 pKa = 4.1TPVGVATTPDD129 pKa = 3.33GLGVYY134 pKa = 7.57VTNNGDD140 pKa = 3.45DD141 pKa = 3.66TVSVIEE147 pKa = 4.13TADD150 pKa = 3.63RR151 pKa = 11.84TVVEE155 pKa = 4.83TIGVGNGPQYY165 pKa = 11.41VAFTPNGSRR174 pKa = 11.84AFVTNALEE182 pKa = 4.47DD183 pKa = 3.85SVSVIEE189 pKa = 4.19TSEE192 pKa = 3.9NTLMNTIRR200 pKa = 11.84VGDD203 pKa = 4.04YY204 pKa = 10.49PRR206 pKa = 11.84GLAVTPDD213 pKa = 3.41GLRR216 pKa = 11.84VYY218 pKa = 9.63VANGGSDD225 pKa = 3.42TVSVIDD231 pKa = 3.72TATDD235 pKa = 3.55TVAQTIPAEE244 pKa = 4.19DD245 pKa = 4.57FPWGLALTPDD255 pKa = 3.87GSRR258 pKa = 11.84AYY260 pKa = 8.85VTNLYY265 pKa = 9.64DD266 pKa = 3.74ASVSVIDD273 pKa = 3.65TATNSVVEE281 pKa = 4.68RR282 pKa = 11.84IRR284 pKa = 11.84VGNYY288 pKa = 8.02PYY290 pKa = 10.95GVAVSPDD297 pKa = 3.35GSPVYY302 pKa = 10.39VVNHH306 pKa = 5.78EE307 pKa = 4.13ASTVSVIADD316 pKa = 4.03GTCFGSFCTSS326 pKa = 2.84

Molecular weight: 33.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S3AKQ9|A0A3S3AKQ9_9NOCA Carboxylic ester hydrolase OS=Rhodococcus sp. LHW51113 OX=2487364 GN=EGT50_09675 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.72GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3281

0

3281

1087130

21

8960

331.3

35.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.554 ± 0.056

0.721 ± 0.011

6.346 ± 0.034

5.781 ± 0.031

2.981 ± 0.027

9.17 ± 0.038

2.042 ± 0.018

4.2 ± 0.028

2.097 ± 0.032

9.859 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.93 ± 0.017

2.089 ± 0.023

5.625 ± 0.035

2.776 ± 0.021

7.337 ± 0.04

5.763 ± 0.027

6.177 ± 0.033

9.169 ± 0.04

1.386 ± 0.019

1.994 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski