Klebsiella phage vB_KleS-HSE3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3H5E0|A0A6M3H5E0_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KleS-HSE3 OX=2713250 GN=HSE3_gp044 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 9.97MIGNLEE8 pKa = 4.08DD9 pKa = 3.83LKK11 pKa = 11.5AFAAANGYY19 pKa = 11.0AGMTDD24 pKa = 3.57EE25 pKa = 4.86EE26 pKa = 5.02LSVSLRR32 pKa = 11.84QANLWAWSLPWKK44 pKa = 10.4GEE46 pKa = 3.94QVEE49 pKa = 4.44PGQPDD54 pKa = 2.89IWPRR58 pKa = 11.84TICDD62 pKa = 4.29DD63 pKa = 4.24YY64 pKa = 12.06NCLDD68 pKa = 4.04YY69 pKa = 9.79DD70 pKa = 4.05TPPEE74 pKa = 4.2VIRR77 pKa = 11.84FVYY80 pKa = 9.61TVVCDD85 pKa = 3.83AVDD88 pKa = 3.25SGTEE92 pKa = 3.96FNTASAGAFKK102 pKa = 10.64KK103 pKa = 10.55RR104 pKa = 11.84VGIDD108 pKa = 3.12GAIDD112 pKa = 3.47VEE114 pKa = 4.5YY115 pKa = 11.01SEE117 pKa = 4.67QSLYY121 pKa = 10.99NAGFEE126 pKa = 4.27APYY129 pKa = 6.97MTGMIGHH136 pKa = 7.48WLDD139 pKa = 3.64GNGRR143 pKa = 11.84GCNIKK148 pKa = 10.49LEE150 pKa = 4.39RR151 pKa = 11.84GG152 pKa = 3.41

Molecular weight: 16.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3H360|A0A6M3H360_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KleS-HSE3 OX=2713250 GN=HSE3_gp009 PE=4 SV=1
MM1 pKa = 7.35QDD3 pKa = 2.54HH4 pKa = 7.93DD5 pKa = 3.93IRR7 pKa = 11.84HH8 pKa = 6.5PFAQPLRR15 pKa = 11.84LLAQKK20 pKa = 10.39ILLPSLAAVLVRR32 pKa = 11.84HH33 pKa = 5.84MEE35 pKa = 4.25PVDD38 pKa = 3.08VWIPVIEE45 pKa = 4.57PGFKK49 pKa = 10.17VNQVVILKK57 pKa = 9.63RR58 pKa = 11.84AYY60 pKa = 9.68RR61 pKa = 11.84IGWRR65 pKa = 11.84EE66 pKa = 3.52QVFFDD71 pKa = 4.6HH72 pKa = 7.32RR73 pKa = 11.84SSPPSKK79 pKa = 9.6ILRR82 pKa = 11.84AITSRR87 pKa = 11.84DD88 pKa = 3.31LFPPMRR94 pKa = 11.84RR95 pKa = 11.84CVTRR99 pKa = 11.84FGAAIRR105 pKa = 11.84CADD108 pKa = 2.9GRR110 pKa = 11.84IFVFTPRR117 pKa = 11.84LIFPLVLGDD126 pKa = 3.93ATCLSSAAFHH136 pKa = 6.4WCFAGILSLSGQNISS151 pKa = 3.49

Molecular weight: 17.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

15336

67

1474

251.4

27.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.146 ± 0.469

1.187 ± 0.154

6.149 ± 0.289

5.425 ± 0.372

3.619 ± 0.233

7.981 ± 0.292

1.663 ± 0.229

5.908 ± 0.198

4.799 ± 0.332

7.388 ± 0.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.179

4.975 ± 0.346

3.912 ± 0.29

4.656 ± 0.295

6.279 ± 0.349

6.266 ± 0.364

6.266 ± 0.56

6.586 ± 0.247

1.474 ± 0.112

2.863 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski