Rickettsia prowazekii (strain Madrid E)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Rickettsiaceae; Rickettsieae; Rickettsia; typhus group; Rickettsia prowazekii

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9ZE10|GATA_RICPR Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=gatA PE=3 SV=1
MM1 pKa = 7.5EE2 pKa = 5.49FCTNTIDD9 pKa = 3.87TISYY13 pKa = 6.28TTHH16 pKa = 6.53EE17 pKa = 4.95SIEE20 pKa = 4.08EE21 pKa = 4.11PNLKK25 pKa = 10.37EE26 pKa = 5.45KK27 pKa = 10.7DD28 pKa = 3.48IEE30 pKa = 4.93DD31 pKa = 4.19DD32 pKa = 3.99LVVVSLIGNN41 pKa = 4.29

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9ZCV6|BCRH_RICPR Uncharacterized transporter RP603 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=RP603 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.43GFRR19 pKa = 11.84ARR21 pKa = 11.84MITATGRR28 pKa = 11.84AILKK32 pKa = 9.81KK33 pKa = 9.59RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84HH40 pKa = 5.49KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

832

0

832

278968

41

2340

335.3

37.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.054 ± 0.071

1.094 ± 0.031

4.84 ± 0.057

5.789 ± 0.08

4.871 ± 0.067

5.428 ± 0.083

1.912 ± 0.034

10.879 ± 0.092

8.378 ± 0.084

10.093 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.165 ± 0.036

6.648 ± 0.115

3.151 ± 0.052

3.144 ± 0.037

3.391 ± 0.053

6.75 ± 0.057

5.213 ± 0.074

5.596 ± 0.062

0.718 ± 0.025

3.885 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski