Klebsiella phage NTUH-K2044-K1-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; Klebsiella virus K244

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068Q6A7|A0A068Q6A7_9CAUD Putative HNH endonuclease OS=Klebsiella phage NTUH-K2044-K1-1 OX=1194091 PE=4 SV=1
MM1 pKa = 7.59DD2 pKa = 4.05QVLDD6 pKa = 4.1AYY8 pKa = 9.93KK9 pKa = 10.48NLAIAVSAVVYY20 pKa = 10.13DD21 pKa = 3.54AAVYY25 pKa = 9.37GVRR28 pKa = 11.84TPRR31 pKa = 11.84LNDD34 pKa = 3.08VYY36 pKa = 11.41DD37 pKa = 4.47ALDD40 pKa = 3.86KK41 pKa = 11.12LAALYY46 pKa = 10.99GMDD49 pKa = 4.95LEE51 pKa = 4.79LAATAFKK58 pKa = 10.44EE59 pKa = 4.24HH60 pKa = 6.94NDD62 pKa = 3.63LAAHH66 pKa = 6.6ADD68 pKa = 3.59KK69 pKa = 11.22LRR71 pKa = 11.84GDD73 pKa = 4.17DD74 pKa = 3.65LVLIRR79 pKa = 11.84VVGTLSIGLAEE90 pKa = 4.81IGSCIYY96 pKa = 10.86DD97 pKa = 3.38ADD99 pKa = 3.65QSLRR103 pKa = 11.84TPEE106 pKa = 4.14VIGDD110 pKa = 3.63VLGTVLVLSEE120 pKa = 4.7LGAA123 pKa = 4.53

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068Q7S2|A0A068Q7S2_9CAUD Putative head-tail connector protein OS=Klebsiella phage NTUH-K2044-K1-1 OX=1194091 PE=4 SV=1
MM1 pKa = 6.38VARR4 pKa = 11.84RR5 pKa = 11.84ITRR8 pKa = 11.84GQVRR12 pKa = 11.84AVTLKK17 pKa = 10.71LLKK20 pKa = 10.26EE21 pKa = 4.01QGGLCLICGKK31 pKa = 9.76PIEE34 pKa = 4.44LTAKK38 pKa = 8.83STSRR42 pKa = 11.84DD43 pKa = 3.45GPALDD48 pKa = 3.69HH49 pKa = 5.75SHH51 pKa = 5.99RR52 pKa = 11.84TGHH55 pKa = 5.83IRR57 pKa = 11.84GVLHH61 pKa = 6.81RR62 pKa = 11.84SCNGGIGRR70 pKa = 11.84AEE72 pKa = 4.01SVIGRR77 pKa = 11.84WVTGSMQDD85 pKa = 2.94EE86 pKa = 4.22AAIVDD91 pKa = 3.67DD92 pKa = 4.05MQRR95 pKa = 11.84MVDD98 pKa = 3.65YY99 pKa = 10.61LRR101 pKa = 11.84KK102 pKa = 9.82PEE104 pKa = 4.16TEE106 pKa = 4.25LIYY109 pKa = 9.54YY110 pKa = 6.16THH112 pKa = 7.49KK113 pKa = 11.05SPEE116 pKa = 4.06EE117 pKa = 3.4LAQAQKK123 pKa = 11.04LKK125 pKa = 10.62ARR127 pKa = 11.84KK128 pKa = 9.05ARR130 pKa = 11.84ARR132 pKa = 11.84RR133 pKa = 11.84KK134 pKa = 9.5ARR136 pKa = 11.84EE137 pKa = 3.88IIKK140 pKa = 10.56

Molecular weight: 15.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

12351

100

1232

352.9

39.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.08 ± 0.687

1.255 ± 0.229

6.218 ± 0.25

5.765 ± 0.424

3.093 ± 0.171

7.57 ± 0.249

1.87 ± 0.192

4.364 ± 0.179

4.477 ± 0.31

8.72 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.963 ± 0.224

3.967 ± 0.227

3.975 ± 0.291

4.769 ± 0.389

6.105 ± 0.278

6.582 ± 0.39

5.805 ± 0.294

7.109 ± 0.31

1.457 ± 0.11

3.854 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski