Dysgonomonas macrotermitis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Dysgonomonadaceae; Dysgonomonas

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4009 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5GYG6|A0A1M5GYG6_9BACT DUF2185 domain-containing protein OS=Dysgonomonas macrotermitis OX=1346286 GN=SAMN05444362_11523 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.6SLVTTLFLSSFILFSVGCSNDD23 pKa = 3.5NNSDD27 pKa = 4.14LIDD30 pKa = 4.32GGTEE34 pKa = 3.62VTGIQQSEE42 pKa = 3.98IKK44 pKa = 10.54LRR46 pKa = 11.84IAEE49 pKa = 4.36YY50 pKa = 10.45YY51 pKa = 10.02SAPSTSAQSVSNIQTEE67 pKa = 4.25FDD69 pKa = 3.64KK70 pKa = 11.2KK71 pKa = 10.68FPNATDD77 pKa = 4.04VEE79 pKa = 4.72WKK81 pKa = 10.47ASNNVYY87 pKa = 10.53EE88 pKa = 4.38IDD90 pKa = 3.9FEE92 pKa = 4.37ISNVDD97 pKa = 3.14YY98 pKa = 10.61EE99 pKa = 4.2AWYY102 pKa = 10.13DD103 pKa = 3.89SNANLLMYY111 pKa = 10.24KK112 pKa = 10.17YY113 pKa = 10.46DD114 pKa = 3.52IANGEE119 pKa = 4.22LPSAVSSAIASDD131 pKa = 3.7YY132 pKa = 10.78TGYY135 pKa = 8.68TLDD138 pKa = 3.91EE139 pKa = 4.33AEE141 pKa = 4.46KK142 pKa = 9.87VYY144 pKa = 10.63KK145 pKa = 10.84GEE147 pKa = 4.2IIGYY151 pKa = 9.43YY152 pKa = 10.26VDD154 pKa = 3.74LKK156 pKa = 10.89KK157 pKa = 10.94NKK159 pKa = 8.75TEE161 pKa = 3.4VHH163 pKa = 6.04AFYY166 pKa = 10.91KK167 pKa = 10.63EE168 pKa = 3.96DD169 pKa = 3.29GTFISKK175 pKa = 9.94NLWEE179 pKa = 5.47DD180 pKa = 4.18DD181 pKa = 3.87SVKK184 pKa = 10.17PANDD188 pKa = 3.83AEE190 pKa = 4.53TSTPEE195 pKa = 3.7ISGTLTDD202 pKa = 4.04EE203 pKa = 4.39ATDD206 pKa = 3.87ALIAAYY212 pKa = 10.54YY213 pKa = 9.97SGNDD217 pKa = 3.4TDD219 pKa = 4.75ILPANVPSAISSNFKK234 pKa = 9.78TVFPNARR241 pKa = 11.84DD242 pKa = 3.75IDD244 pKa = 4.05WEE246 pKa = 4.4TSANVYY252 pKa = 10.4KK253 pKa = 10.73VDD255 pKa = 4.23FEE257 pKa = 5.16INNVDD262 pKa = 2.88YY263 pKa = 10.88DD264 pKa = 3.06AWYY267 pKa = 10.84NSDD270 pKa = 3.41GTLLAYY276 pKa = 10.41RR277 pKa = 11.84FDD279 pKa = 3.71ITKK282 pKa = 10.46SSLPQSVQTAISDD295 pKa = 3.66KK296 pKa = 10.64FNGYY300 pKa = 8.01TIEE303 pKa = 4.58DD304 pKa = 3.64VEE306 pKa = 4.63KK307 pKa = 10.83VIKK310 pKa = 10.59ANSAGYY316 pKa = 9.87LVEE319 pKa = 5.06LEE321 pKa = 4.13NLNIEE326 pKa = 3.78EE327 pKa = 4.24DD328 pKa = 3.92AYY330 pKa = 10.14FTEE333 pKa = 5.21DD334 pKa = 2.59GTYY337 pKa = 10.17ISNSFYY343 pKa = 10.8KK344 pKa = 10.75ASTSGDD350 pKa = 3.4NTEE353 pKa = 4.65EE354 pKa = 4.08PVTTPEE360 pKa = 3.91IPVDD364 pKa = 3.66GNYY367 pKa = 10.01TDD369 pKa = 5.81DD370 pKa = 5.59EE371 pKa = 5.0IDD373 pKa = 4.02ALLASYY379 pKa = 9.88QQGRR383 pKa = 11.84DD384 pKa = 3.12KK385 pKa = 11.01DD386 pKa = 3.58INAANVPVLVTTAFNSQFASARR408 pKa = 11.84DD409 pKa = 3.56IDD411 pKa = 3.69WDD413 pKa = 4.04YY414 pKa = 11.59VGNVYY419 pKa = 10.56KK420 pKa = 10.68VDD422 pKa = 4.07FEE424 pKa = 4.35IANVDD429 pKa = 3.4YY430 pKa = 10.29EE431 pKa = 4.12AWYY434 pKa = 10.88VSDD437 pKa = 4.21GTLLMYY443 pKa = 8.92TQEE446 pKa = 3.84VRR448 pKa = 11.84YY449 pKa = 7.59PTIASTVQNAVAAKK463 pKa = 7.44YY464 pKa = 9.05TEE466 pKa = 4.15YY467 pKa = 11.07KK468 pKa = 10.53VDD470 pKa = 3.5GCDD473 pKa = 3.35YY474 pKa = 9.91FQKK477 pKa = 10.19GTIKK481 pKa = 10.72GYY483 pKa = 10.26IIEE486 pKa = 4.73LEE488 pKa = 4.2NKK490 pKa = 8.79RR491 pKa = 11.84TDD493 pKa = 3.04AEE495 pKa = 4.53LIVIYY500 pKa = 10.64KK501 pKa = 10.02EE502 pKa = 3.95DD503 pKa = 3.51GAFISEE509 pKa = 4.08ITDD512 pKa = 3.46YY513 pKa = 11.75

Molecular weight: 57.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4X4T7|A0A1M4X4T7_9BACT TonB-linked outer membrane protein SusC/RagA family OS=Dysgonomonas macrotermitis OX=1346286 GN=SAMN05444362_102396 PE=3 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84TSRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 7.8GFRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84FRR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 6.62RR21 pKa = 11.84TRR23 pKa = 11.84FRR25 pKa = 11.84GYY27 pKa = 9.0FRR29 pKa = 11.84RR30 pKa = 11.84FRR32 pKa = 11.84SFRR35 pKa = 11.84PFRR38 pKa = 11.84RR39 pKa = 11.84FRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84SSSGRR49 pKa = 11.84GLFSGRR55 pKa = 11.84RR56 pKa = 11.84TRR58 pKa = 11.84ILGFGVPTILLVLAVIVAIWKK79 pKa = 6.33WQPIKK84 pKa = 9.83TWVSKK89 pKa = 10.19FIKK92 pKa = 10.31VV93 pKa = 3.18

Molecular weight: 11.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4009

0

4009

1390717

28

2492

346.9

39.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.359 ± 0.04

0.955 ± 0.013

5.834 ± 0.026

6.184 ± 0.044

4.717 ± 0.029

6.584 ± 0.04

1.618 ± 0.018

7.929 ± 0.041

7.142 ± 0.037

8.945 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.016

5.817 ± 0.034

3.522 ± 0.02

3.358 ± 0.019

3.961 ± 0.025

6.837 ± 0.033

5.828 ± 0.037

6.133 ± 0.029

1.228 ± 0.016

4.682 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski