Proteome-pI 2.0 - Proteome Isoelectric Point Database

Beihai barnacle virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.81

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KI52|A0A1L3KI52_9VIRU RNA-dependent RNA polymerase OS=Beihai barnacle virus 10 OX=1922354 PE=4 SV=1

MM1 pKa = 7.5QSTNLLTSKK10 pKa = 10.48LIQTGCPRR18 pKa = 11.84APAGKK23 pKa = 9.79LGRR26 pKa = 11.84GYY28 pKa = 10.68RR29 pKa = 11.84IFIPHH34 pKa = 7.37LYY36 pKa = 10.6EE37 pKa = 5.34NGDD40 pKa = 3.51YY41 pKa = 11.07LLPGRR46 pKa = 11.84NHH48 pKa = 6.47SLLKK52 pKa = 10.64GVVVRR57 pKa = 11.84NRR59 pKa = 11.84MVKK62 pKa = 10.26PNLLISKK69 pKa = 7.68LTLMGFGKK77 pKa = 10.56RR78 pKa = 11.84FATLVSLRR86 pKa = 11.84PISHH90 pKa = 7.08IEE92 pKa = 3.72RR93 pKa = 11.84AEE95 pKa = 3.81QSVLGVIDD103 pKa = 4.49SLLLYY108 pKa = 10.53DD109 pKa = 4.46SEE111 pKa = 5.89LFITDD116 pKa = 3.27TGYY119 pKa = 11.76KK120 pKa = 9.54LLKK123 pKa = 10.45FIVKK127 pKa = 8.52KK128 pKa = 7.68TFKK131 pKa = 10.79VAIFNCALVTKK142 pKa = 9.64LWKK145 pKa = 10.37EE146 pKa = 3.37FSFYY150 pKa = 10.19VWAHH154 pKa = 3.91VTEE157 pKa = 4.55TVTRR161 pKa = 11.84EE162 pKa = 3.55KK163 pKa = 10.47PVRR166 pKa = 11.84KK167 pKa = 9.54RR168 pKa = 11.84EE169 pKa = 3.85NFFFSLVSEE178 pKa = 4.15QDD180 pKa = 3.14IEE182 pKa = 4.55VLKK185 pKa = 9.98LTLNKK190 pKa = 8.97KK191 pKa = 7.07TLTRR195 pKa = 11.84LAHH198 pKa = 5.65LTSSRR203 pKa = 11.84QFPPGEE209 pKa = 3.8RR210 pKa = 11.84QQEE213 pKa = 4.17IKK215 pKa = 10.49SLKK218 pKa = 10.3DD219 pKa = 3.36FEE221 pKa = 4.78SHH223 pKa = 4.46VTEE226 pKa = 4.87PYY228 pKa = 9.81IGNPVFLRR236 pKa = 11.84RR237 pKa = 11.84IRR239 pKa = 11.84LASRR243 pKa = 11.84VVGRR247 pKa = 11.84KK248 pKa = 8.7ARR250 pKa = 11.84RR251 pKa = 11.84LGMKK255 pKa = 9.86PLTDD259 pKa = 3.27SHH261 pKa = 7.22LSLAAAGSFYY271 pKa = 10.81TSVKK275 pKa = 10.47DD276 pKa = 3.41GGRR279 pKa = 11.84AEE281 pKa = 4.49EE282 pKa = 4.25LLNMLFKK289 pKa = 11.06YY290 pKa = 10.24LAYY293 pKa = 10.28VPKK296 pKa = 9.73EE297 pKa = 3.77SCEE300 pKa = 3.92IKK302 pKa = 10.11TPFYY306 pKa = 10.0TLKK309 pKa = 10.77DD310 pKa = 3.68VEE312 pKa = 4.76GVEE315 pKa = 5.31RR316 pKa = 11.84WRR318 pKa = 11.84TWCRR322 pKa = 11.84PAVYY326 pKa = 10.49DD327 pKa = 3.71HH328 pKa = 6.83FPNVSFGNLLPEE340 pKa = 4.05TLMGFEE346 pKa = 4.68TYY348 pKa = 10.52YY349 pKa = 10.72QGFDD353 pKa = 4.31EE354 pKa = 4.42ILGMQILACSYY365 pKa = 10.8LEE367 pKa = 3.99QEE369 pKa = 4.46EE370 pKa = 4.46HH371 pKa = 6.8LKK373 pKa = 10.9CNQNIPVRR381 pKa = 11.84VLTITEE387 pKa = 4.5PGSKK391 pKa = 10.25ARR393 pKa = 11.84IVTTGPCWLYY403 pKa = 10.24TLQQPVSHH411 pKa = 6.53VLRR414 pKa = 11.84GFLGQHH420 pKa = 6.55PSAAAGLTMSDD431 pKa = 3.75QAWQFLFLLEE441 pKa = 4.21KK442 pKa = 10.58AKK444 pKa = 10.87SHH446 pKa = 6.75FGDD449 pKa = 4.67DD450 pKa = 4.87FSCLSSDD457 pKa = 3.35LTAATDD463 pKa = 4.02VIPLCILKK471 pKa = 10.03EE472 pKa = 3.98LYY474 pKa = 10.41EE475 pKa = 4.48GFLEE479 pKa = 4.29GLRR482 pKa = 11.84IEE484 pKa = 5.27GPFLNIVGKK493 pKa = 9.46MIQLPRR499 pKa = 11.84LCSVEE504 pKa = 4.34KK505 pKa = 10.53INSYY509 pKa = 10.42FLNSRR514 pKa = 11.84GIFMGEE520 pKa = 4.28PIAKK524 pKa = 9.84VLLTLLNLSCEE535 pKa = 4.43EE536 pKa = 3.81IAIRR540 pKa = 11.84DD541 pKa = 3.71YY542 pKa = 11.58LGVDD546 pKa = 4.16FRR548 pKa = 11.84ASVQVRR554 pKa = 11.84WRR556 pKa = 11.84CFSVAGDD563 pKa = 3.45DD564 pKa = 4.55HH565 pKa = 7.63IAVGPVGYY573 pKa = 9.94LDD575 pKa = 5.56KK576 pKa = 10.6ITEE579 pKa = 4.17THH581 pKa = 6.06IKK583 pKa = 10.04AGSKK587 pKa = 10.13ISDD590 pKa = 3.76LKK592 pKa = 10.72HH593 pKa = 4.4GHH595 pKa = 5.29SRR597 pKa = 11.84IAVRR601 pKa = 11.84YY602 pKa = 8.23CEE604 pKa = 4.93KK605 pKa = 10.59ILDD608 pKa = 3.59VRR610 pKa = 11.84NFKK613 pKa = 10.91GSWSKK618 pKa = 9.18YY619 pKa = 7.59TINDD623 pKa = 3.16STEE626 pKa = 4.05AYY628 pKa = 9.56LASPFVDD635 pKa = 4.02SIKK638 pKa = 10.59VRR640 pKa = 11.84LLSPAAKK647 pKa = 9.98NVLSFNEE654 pKa = 4.01KK655 pKa = 8.53NTAFGKK661 pKa = 10.48GKK663 pKa = 10.43SLGRR667 pKa = 11.84TLQWLNKK674 pKa = 10.31DD675 pKa = 3.83CFDD678 pKa = 4.43SKK680 pKa = 10.44WISLVRR686 pKa = 11.84DD687 pKa = 3.33WFFKK691 pKa = 10.67RR692 pKa = 11.84MSSLLPDD699 pKa = 4.03RR700 pKa = 11.84SSGVYY705 pKa = 7.5WHH707 pKa = 7.08LLLPEE712 pKa = 4.27HH713 pKa = 6.82LGGLGLGTEE722 pKa = 4.71RR723 pKa = 11.84DD724 pKa = 3.85FEE726 pKa = 4.47DD727 pKa = 5.18LIVRR731 pKa = 11.84LPSPSRR737 pKa = 11.84TLLKK741 pKa = 10.58SIEE744 pKa = 4.5DD745 pKa = 3.74GNPNMGHH752 pKa = 6.26IRR754 pKa = 11.84LFSGFTSNTTYY765 pKa = 10.49RR766 pKa = 11.84GYY768 pKa = 10.82KK769 pKa = 9.45IPEE772 pKa = 4.09TEE774 pKa = 4.17KK775 pKa = 10.86EE776 pKa = 3.65IAALFFQGILEE787 pKa = 4.25EE788 pKa = 4.18EE789 pKa = 4.42KK790 pKa = 10.67SDD792 pKa = 4.11KK793 pKa = 11.02FEE795 pKa = 4.59NLVKK799 pKa = 10.64LYY801 pKa = 10.05RR802 pKa = 11.84YY803 pKa = 9.52EE804 pKa = 4.57DD805 pKa = 5.22LIPNTAIRR813 pKa = 11.84LLRR816 pKa = 11.84TQGWVRR822 pKa = 11.84KK823 pKa = 9.81DD824 pKa = 3.49YY825 pKa = 11.7LEE827 pKa = 4.9DD828 pKa = 4.71KK829 pKa = 10.33ILRR832 pKa = 11.84PFLFKK837 pKa = 10.74EE838 pKa = 3.9ILTGRR843 pKa = 11.84AKK845 pKa = 10.54VKK847 pKa = 10.73AFNTEE852 pKa = 4.05HH853 pKa = 6.64LKK855 pKa = 10.68SRR857 pKa = 11.84YY858 pKa = 9.12ARR860 pKa = 11.84LWDD863 pKa = 3.19LTYY866 pKa = 10.62RR867 pKa = 11.84GEE869 pKa = 4.23EE870 pKa = 4.19TISPQTLQVCFKK882 pKa = 9.79PPAYY886 pKa = 9.91HH887 pKa = 7.26LYY889 pKa = 10.59YY890 pKa = 11.2KK891 pKa = 10.14MGEE894 pKa = 4.06KK895 pKa = 10.61LEE897 pKa = 4.15LPFRR901 pKa = 11.84GRR903 pKa = 11.84VLKK906 pKa = 11.15VNLLEE911 pKa = 4.44EE912 pKa = 4.85GLLGMPDD919 pKa = 4.6LSIPWEE925 pKa = 4.21VIGDD929 pKa = 3.73ISGPLAPKK937 pKa = 10.02RR938 pKa = 11.84GNHH941 pKa = 5.12ARR943 pKa = 11.84SRR945 pKa = 11.84VIKK948 pKa = 10.2SPRR951 pKa = 11.84RR952 pKa = 11.84RR953 pKa = 11.84NSS955 pKa = 2.7

Molecular weight: 109.03 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KI52|A0A1L3KI52_9VIRU RNA-dependent RNA polymerase OS=Beihai barnacle virus 10 OX=1922354 PE=4 SV=1

MM1 pKa = 7.5QSTNLLTSKK10 pKa = 10.48LIQTGCPRR18 pKa = 11.84APAGKK23 pKa = 9.79LGRR26 pKa = 11.84GYY28 pKa = 10.68RR29 pKa = 11.84IFIPHH34 pKa = 7.37LYY36 pKa = 10.6EE37 pKa = 5.34NGDD40 pKa = 3.51YY41 pKa = 11.07LLPGRR46 pKa = 11.84NHH48 pKa = 6.47SLLKK52 pKa = 10.64GVVVRR57 pKa = 11.84NRR59 pKa = 11.84MVKK62 pKa = 10.26PNLLISKK69 pKa = 7.68LTLMGFGKK77 pKa = 10.56RR78 pKa = 11.84FATLVSLRR86 pKa = 11.84PISHH90 pKa = 7.08IEE92 pKa = 3.72RR93 pKa = 11.84AEE95 pKa = 3.81QSVLGVIDD103 pKa = 4.49SLLLYY108 pKa = 10.53DD109 pKa = 4.46SEE111 pKa = 5.89LFITDD116 pKa = 3.27TGYY119 pKa = 11.76KK120 pKa = 9.54LLKK123 pKa = 10.45FIVKK127 pKa = 8.52KK128 pKa = 7.68TFKK131 pKa = 10.79VAIFNCALVTKK142 pKa = 9.64LWKK145 pKa = 10.37EE146 pKa = 3.37FSFYY150 pKa = 10.19VWAHH154 pKa = 3.91VTEE157 pKa = 4.55TVTRR161 pKa = 11.84EE162 pKa = 3.55KK163 pKa = 10.47PVRR166 pKa = 11.84KK167 pKa = 9.54RR168 pKa = 11.84EE169 pKa = 3.85NFFFSLVSEE178 pKa = 4.15QDD180 pKa = 3.14IEE182 pKa = 4.55VLKK185 pKa = 9.98LTLNKK190 pKa = 8.97KK191 pKa = 7.07TLTRR195 pKa = 11.84LAHH198 pKa = 5.65LTSSRR203 pKa = 11.84QFPPGEE209 pKa = 3.8RR210 pKa = 11.84QQEE213 pKa = 4.17IKK215 pKa = 10.49SLKK218 pKa = 10.3DD219 pKa = 3.36FEE221 pKa = 4.78SHH223 pKa = 4.46VTEE226 pKa = 4.87PYY228 pKa = 9.81IGNPVFLRR236 pKa = 11.84RR237 pKa = 11.84IRR239 pKa = 11.84LASRR243 pKa = 11.84VVGRR247 pKa = 11.84KK248 pKa = 8.7ARR250 pKa = 11.84RR251 pKa = 11.84LGMKK255 pKa = 9.86PLTDD259 pKa = 3.27SHH261 pKa = 7.22LSLAAAGSFYY271 pKa = 10.81TSVKK275 pKa = 10.47DD276 pKa = 3.41GGRR279 pKa = 11.84AEE281 pKa = 4.49EE282 pKa = 4.25LLNMLFKK289 pKa = 11.06YY290 pKa = 10.24LAYY293 pKa = 10.28VPKK296 pKa = 9.73EE297 pKa = 3.77SCEE300 pKa = 3.92IKK302 pKa = 10.11TPFYY306 pKa = 10.0TLKK309 pKa = 10.77DD310 pKa = 3.68VEE312 pKa = 4.76GVEE315 pKa = 5.31RR316 pKa = 11.84WRR318 pKa = 11.84TWCRR322 pKa = 11.84PAVYY326 pKa = 10.49DD327 pKa = 3.71HH328 pKa = 6.83FPNVSFGNLLPEE340 pKa = 4.05TLMGFEE346 pKa = 4.68TYY348 pKa = 10.52YY349 pKa = 10.72QGFDD353 pKa = 4.31EE354 pKa = 4.42ILGMQILACSYY365 pKa = 10.8LEE367 pKa = 3.99QEE369 pKa = 4.46EE370 pKa = 4.46HH371 pKa = 6.8LKK373 pKa = 10.9CNQNIPVRR381 pKa = 11.84VLTITEE387 pKa = 4.5PGSKK391 pKa = 10.25ARR393 pKa = 11.84IVTTGPCWLYY403 pKa = 10.24TLQQPVSHH411 pKa = 6.53VLRR414 pKa = 11.84GFLGQHH420 pKa = 6.55PSAAAGLTMSDD431 pKa = 3.75QAWQFLFLLEE441 pKa = 4.21KK442 pKa = 10.58AKK444 pKa = 10.87SHH446 pKa = 6.75FGDD449 pKa = 4.67DD450 pKa = 4.87FSCLSSDD457 pKa = 3.35LTAATDD463 pKa = 4.02VIPLCILKK471 pKa = 10.03EE472 pKa = 3.98LYY474 pKa = 10.41EE475 pKa = 4.48GFLEE479 pKa = 4.29GLRR482 pKa = 11.84IEE484 pKa = 5.27GPFLNIVGKK493 pKa = 9.46MIQLPRR499 pKa = 11.84LCSVEE504 pKa = 4.34KK505 pKa = 10.53INSYY509 pKa = 10.42FLNSRR514 pKa = 11.84GIFMGEE520 pKa = 4.28PIAKK524 pKa = 9.84VLLTLLNLSCEE535 pKa = 4.43EE536 pKa = 3.81IAIRR540 pKa = 11.84DD541 pKa = 3.71YY542 pKa = 11.58LGVDD546 pKa = 4.16FRR548 pKa = 11.84ASVQVRR554 pKa = 11.84WRR556 pKa = 11.84CFSVAGDD563 pKa = 3.45DD564 pKa = 4.55HH565 pKa = 7.63IAVGPVGYY573 pKa = 9.94LDD575 pKa = 5.56KK576 pKa = 10.6ITEE579 pKa = 4.17THH581 pKa = 6.06IKK583 pKa = 10.04AGSKK587 pKa = 10.13ISDD590 pKa = 3.76LKK592 pKa = 10.72HH593 pKa = 4.4GHH595 pKa = 5.29SRR597 pKa = 11.84IAVRR601 pKa = 11.84YY602 pKa = 8.23CEE604 pKa = 4.93KK605 pKa = 10.59ILDD608 pKa = 3.59VRR610 pKa = 11.84NFKK613 pKa = 10.91GSWSKK618 pKa = 9.18YY619 pKa = 7.59TINDD623 pKa = 3.16STEE626 pKa = 4.05AYY628 pKa = 9.56LASPFVDD635 pKa = 4.02SIKK638 pKa = 10.59VRR640 pKa = 11.84LLSPAAKK647 pKa = 9.98NVLSFNEE654 pKa = 4.01KK655 pKa = 8.53NTAFGKK661 pKa = 10.48GKK663 pKa = 10.43SLGRR667 pKa = 11.84TLQWLNKK674 pKa = 10.31DD675 pKa = 3.83CFDD678 pKa = 4.43SKK680 pKa = 10.44WISLVRR686 pKa = 11.84DD687 pKa = 3.33WFFKK691 pKa = 10.67RR692 pKa = 11.84MSSLLPDD699 pKa = 4.03RR700 pKa = 11.84SSGVYY705 pKa = 7.5WHH707 pKa = 7.08LLLPEE712 pKa = 4.27HH713 pKa = 6.82LGGLGLGTEE722 pKa = 4.71RR723 pKa = 11.84DD724 pKa = 3.85FEE726 pKa = 4.47DD727 pKa = 5.18LIVRR731 pKa = 11.84LPSPSRR737 pKa = 11.84TLLKK741 pKa = 10.58SIEE744 pKa = 4.5DD745 pKa = 3.74GNPNMGHH752 pKa = 6.26IRR754 pKa = 11.84LFSGFTSNTTYY765 pKa = 10.49RR766 pKa = 11.84GYY768 pKa = 10.82KK769 pKa = 9.45IPEE772 pKa = 4.09TEE774 pKa = 4.17KK775 pKa = 10.86EE776 pKa = 3.65IAALFFQGILEE787 pKa = 4.25EE788 pKa = 4.18EE789 pKa = 4.42KK790 pKa = 10.67SDD792 pKa = 4.11KK793 pKa = 11.02FEE795 pKa = 4.59NLVKK799 pKa = 10.64LYY801 pKa = 10.05RR802 pKa = 11.84YY803 pKa = 9.52EE804 pKa = 4.57DD805 pKa = 5.22LIPNTAIRR813 pKa = 11.84LLRR816 pKa = 11.84TQGWVRR822 pKa = 11.84KK823 pKa = 9.81DD824 pKa = 3.49YY825 pKa = 11.7LEE827 pKa = 4.9DD828 pKa = 4.71KK829 pKa = 10.33ILRR832 pKa = 11.84PFLFKK837 pKa = 10.74EE838 pKa = 3.9ILTGRR843 pKa = 11.84AKK845 pKa = 10.54VKK847 pKa = 10.73AFNTEE852 pKa = 4.05HH853 pKa = 6.64LKK855 pKa = 10.68SRR857 pKa = 11.84YY858 pKa = 9.12ARR860 pKa = 11.84LWDD863 pKa = 3.19LTYY866 pKa = 10.62RR867 pKa = 11.84GEE869 pKa = 4.23EE870 pKa = 4.19TISPQTLQVCFKK882 pKa = 9.79PPAYY886 pKa = 9.91HH887 pKa = 7.26LYY889 pKa = 10.59YY890 pKa = 11.2KK891 pKa = 10.14MGEE894 pKa = 4.06KK895 pKa = 10.61LEE897 pKa = 4.15LPFRR901 pKa = 11.84GRR903 pKa = 11.84VLKK906 pKa = 11.15VNLLEE911 pKa = 4.44EE912 pKa = 4.85GLLGMPDD919 pKa = 4.6LSIPWEE925 pKa = 4.21VIGDD929 pKa = 3.73ISGPLAPKK937 pKa = 10.02RR938 pKa = 11.84GNHH941 pKa = 5.12ARR943 pKa = 11.84SRR945 pKa = 11.84VIKK948 pKa = 10.2SPRR951 pKa = 11.84RR952 pKa = 11.84RR953 pKa = 11.84NSS955 pKa = 2.7

Molecular weight: 109.03 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	955	955	955	955.0	109.03

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
4.817 ± 0.0	1.571 ± 0.0	4.084 ± 0.0	6.492 ± 0.0	5.34 ± 0.0	6.806 ± 0.0	2.304 ± 0.0	5.654 ± 0.0	7.12 ± 0.0	13.089 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.466 ± 0.0	3.455 ± 0.0	4.817 ± 0.0	2.408 ± 0.0	6.911 ± 0.0	7.12 ± 0.0	5.445 ± 0.0	5.969 ± 0.0	1.571 ± 0.0	3.56 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski