Shahe isopoda virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KL89|A0A1L3KL89_9VIRU Uncharacterized protein OS=Shahe isopoda virus 3 OX=1923423 PE=4 SV=1
MM1 pKa = 7.55AAPVGANYY9 pKa = 7.49FTQAALNTLAYY20 pKa = 9.94VAGIPGLDD28 pKa = 3.49PRR30 pKa = 11.84DD31 pKa = 4.74LIMQGHH37 pKa = 7.07PEE39 pKa = 3.91LFEE42 pKa = 4.24YY43 pKa = 10.48EE44 pKa = 4.49CLALPGMTCGEE55 pKa = 4.18SALRR59 pKa = 11.84LASAAVWSEE68 pKa = 3.85TNLKK72 pKa = 10.13KK73 pKa = 10.34KK74 pKa = 10.38RR75 pKa = 11.84QDD77 pKa = 3.13RR78 pKa = 11.84DD79 pKa = 3.41PPPFLLHH86 pKa = 6.23GVAGALTTLQMVRR99 pKa = 11.84RR100 pKa = 11.84LNRR103 pKa = 11.84LSMGSFPIYY112 pKa = 9.95RR113 pKa = 11.84YY114 pKa = 10.44AGLDD118 pKa = 3.32VLGAPMFDD126 pKa = 4.79LISGKK131 pKa = 9.25PRR133 pKa = 11.84ADD135 pKa = 3.17NYY137 pKa = 11.26ACVFVPQNLVLVPAFPVAHH156 pKa = 5.82WTFTTILPPPDD167 pKa = 3.33VPDD170 pKa = 3.75RR171 pKa = 11.84LVVGLPTLIYY181 pKa = 10.03TRR183 pKa = 11.84LPLAPQMDD191 pKa = 4.69PVPGQIVFWRR201 pKa = 11.84ARR203 pKa = 11.84VTPEE207 pKa = 3.41NAEE210 pKa = 3.98AFAAAAAVGVACKK223 pKa = 10.51CPWIQVCDD231 pKa = 4.09HH232 pKa = 5.8EE233 pKa = 4.75QAWLAVNPFFSRR245 pKa = 11.84VTCADD250 pKa = 3.64SACMEE255 pKa = 4.27GSSYY259 pKa = 11.87AMVLKK264 pKa = 9.61TLSPDD269 pKa = 4.22FILRR273 pKa = 11.84RR274 pKa = 11.84IPNNGVKK281 pKa = 9.92VLQHH285 pKa = 4.82NTVLKK290 pKa = 9.67EE291 pKa = 4.05EE292 pKa = 4.41NVFGCPTIRR301 pKa = 11.84SEE303 pKa = 5.14RR304 pKa = 11.84FFNTLATAFGSLEE317 pKa = 3.98LQPYY321 pKa = 9.43QSVAEE326 pKa = 4.0RR327 pKa = 11.84DD328 pKa = 3.08IYY330 pKa = 10.29PYY332 pKa = 10.48RR333 pKa = 11.84FRR335 pKa = 11.84VYY337 pKa = 9.24TYY339 pKa = 10.05GRR341 pKa = 11.84CVEE344 pKa = 4.27HH345 pKa = 5.83ATMYY349 pKa = 11.68NMFKK353 pKa = 9.83TLCLDD358 pKa = 3.66TASMLSPYY366 pKa = 9.18TFQRR370 pKa = 11.84EE371 pKa = 4.24FEE373 pKa = 4.37VEE375 pKa = 3.99PVAPAKK381 pKa = 10.26PWGEE385 pKa = 4.21CGDD388 pKa = 3.7LTLRR392 pKa = 11.84LPRR395 pKa = 11.84LNEE398 pKa = 3.66LLQRR402 pKa = 11.84LAVRR406 pKa = 11.84KK407 pKa = 8.99EE408 pKa = 3.63VTADD412 pKa = 3.43VVIDD416 pKa = 3.59TVRR419 pKa = 11.84RR420 pKa = 11.84LAHH423 pKa = 5.31EE424 pKa = 4.54EE425 pKa = 3.73KK426 pKa = 8.48WTTEE430 pKa = 3.1IDD432 pKa = 3.45RR433 pKa = 11.84DD434 pKa = 4.04EE435 pKa = 4.72FEE437 pKa = 4.48LWLSRR442 pKa = 11.84VVTEE446 pKa = 4.3AGEE449 pKa = 4.09RR450 pKa = 11.84AQPLTLAYY458 pKa = 9.78TNGQCWTCGTFGKK471 pKa = 7.08THH473 pKa = 5.6RR474 pKa = 11.84HH475 pKa = 4.33EE476 pKa = 4.95CKK478 pKa = 9.4ACKK481 pKa = 9.55RR482 pKa = 11.84RR483 pKa = 11.84ARR485 pKa = 11.84NRR487 pKa = 11.84VPDD490 pKa = 4.11RR491 pKa = 11.84PLSDD495 pKa = 4.21AFATHH500 pKa = 6.35MGFFPLWSEE509 pKa = 4.3RR510 pKa = 11.84FMLPNMAFKK519 pKa = 10.7RR520 pKa = 11.84GTQVRR525 pKa = 11.84VGKK528 pKa = 10.42AGNRR532 pKa = 11.84VMTTRR537 pKa = 11.84AEE539 pKa = 4.09VNAWLSRR546 pKa = 11.84QRR548 pKa = 11.84VDD550 pKa = 2.94VSQRR554 pKa = 11.84GRR556 pKa = 11.84LCGPMFCNQVPKK568 pKa = 10.43CYY570 pKa = 9.92PKK572 pKa = 10.44GQAVALSAFLVRR584 pKa = 11.84LGGTRR589 pKa = 11.84PYY591 pKa = 10.54PDD593 pKa = 3.6TFPCQMCASDD603 pKa = 3.58GHH605 pKa = 5.7EE606 pKa = 4.2EE607 pKa = 4.23CTKK610 pKa = 10.64QCTPDD615 pKa = 3.47EE616 pKa = 4.45LVFAPNNVRR625 pKa = 11.84PEE627 pKa = 3.79VKK629 pKa = 10.07LAYY632 pKa = 9.95DD633 pKa = 3.66LLYY636 pKa = 10.4TVFSQYY642 pKa = 10.86VLYY645 pKa = 10.92GGLTSASCPLKK656 pKa = 10.53PEE658 pKa = 3.84SRR660 pKa = 11.84TEE662 pKa = 4.01FLSHH666 pKa = 7.04FAGEE670 pKa = 4.46KK671 pKa = 8.52LLKK674 pKa = 9.71MVEE677 pKa = 4.06ALAMINDD684 pKa = 3.71GWLEE688 pKa = 3.97NMDD691 pKa = 4.21DD692 pKa = 3.42EE693 pKa = 4.61AVRR696 pKa = 11.84VRR698 pKa = 11.84FTGFTKK704 pKa = 10.62AEE706 pKa = 3.7KK707 pKa = 10.49SYY709 pKa = 11.01DD710 pKa = 3.74FEE712 pKa = 4.47WTGDD716 pKa = 3.66CLILKK721 pKa = 10.22KK722 pKa = 10.28EE723 pKa = 3.98GKK725 pKa = 9.28PRR727 pKa = 11.84FICSPDD733 pKa = 3.4PVMLYY738 pKa = 10.05YY739 pKa = 10.86LGRR742 pKa = 11.84YY743 pKa = 5.89THH745 pKa = 6.55VQTKK749 pKa = 8.5WLSARR754 pKa = 11.84FLPCSRR760 pKa = 11.84MFYY763 pKa = 10.22AGCATPEE770 pKa = 4.48DD771 pKa = 3.92MNVWLNYY778 pKa = 8.27TAGDD782 pKa = 3.73AQYY785 pKa = 10.46MNTIVDD791 pKa = 4.42DD792 pKa = 4.29VSAMDD797 pKa = 3.75SSFTKK802 pKa = 10.24TLLDD806 pKa = 3.3FHH808 pKa = 6.69EE809 pKa = 5.68RR810 pKa = 11.84IRR812 pKa = 11.84DD813 pKa = 3.48LQFPHH818 pKa = 6.31MAAHH822 pKa = 6.41TKK824 pKa = 9.84AAFRR828 pKa = 11.84GEE830 pKa = 3.58EE831 pKa = 4.14RR832 pKa = 11.84FWVRR836 pKa = 11.84IGEE839 pKa = 3.83IRR841 pKa = 11.84AFVEE845 pKa = 4.1WVNASGVPDD854 pKa = 3.7TSYY857 pKa = 11.76KK858 pKa = 9.03NTAPCLPLRR867 pKa = 11.84VFAIVHH873 pKa = 6.78AIWGMIGQPFEE884 pKa = 4.2LTIEE888 pKa = 4.34RR889 pKa = 11.84YY890 pKa = 9.95YY891 pKa = 11.31LVLDD895 pKa = 4.86GIYY898 pKa = 10.17TSAAGDD904 pKa = 3.79DD905 pKa = 3.85GLTRR909 pKa = 11.84VPTHH913 pKa = 5.89MFGVDD918 pKa = 3.1TSSPQFKK925 pKa = 9.69MGYY928 pKa = 8.31CKK930 pKa = 10.26AWSFFGFNVKK940 pKa = 9.79VDD942 pKa = 3.6IVPPCRR948 pKa = 11.84WRR950 pKa = 11.84MATYY954 pKa = 10.06LAQRR958 pKa = 11.84PVWVGHH964 pKa = 5.75RR965 pKa = 11.84YY966 pKa = 8.37EE967 pKa = 4.4WAPEE971 pKa = 3.46PARR974 pKa = 11.84RR975 pKa = 11.84LRR977 pKa = 11.84GIFWQIDD984 pKa = 3.77CSLHH988 pKa = 5.63PIAWGRR994 pKa = 11.84GIATQLLQQAKK1005 pKa = 8.68ALPVVSHH1012 pKa = 5.55VCQWYY1017 pKa = 9.05LARR1020 pKa = 11.84TSGPAIISAATAASHH1035 pKa = 6.57EE1036 pKa = 4.18YY1037 pKa = 10.78SPFHH1041 pKa = 6.49GAVCSGDD1048 pKa = 3.15INEE1051 pKa = 4.66RR1052 pKa = 11.84GVSEE1056 pKa = 4.21FCVDD1060 pKa = 3.43YY1061 pKa = 10.93NIPRR1065 pKa = 11.84AEE1067 pKa = 4.15LDD1069 pKa = 3.4RR1070 pKa = 11.84FCRR1073 pKa = 11.84LLEE1076 pKa = 4.09LVPSVLVNLNSFVLDD1091 pKa = 4.21RR1092 pKa = 11.84IYY1094 pKa = 11.24AEE1096 pKa = 4.3EE1097 pKa = 4.06SS1098 pKa = 3.15

Molecular weight:
123.92 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KL89|A0A1L3KL89_9VIRU Uncharacterized protein OS=Shahe isopoda virus 3 OX=1923423 PE=4 SV=1
MM1 pKa = 7.73PSSKK5 pKa = 10.11PSQLDD10 pKa = 3.68LVSLPTLCKK19 pKa = 10.03TISLPGDD26 pKa = 3.69YY27 pKa = 10.5PPVRR31 pKa = 11.84LPTYY35 pKa = 9.07PAVEE39 pKa = 4.22RR40 pKa = 11.84TSVLPFNATLTTTVPSEE57 pKa = 4.07GMRR60 pKa = 11.84GMLIKK65 pKa = 10.67SSTMPLWTDD74 pKa = 3.4APVVAPYY81 pKa = 10.16SYY83 pKa = 11.31TMGWDD88 pKa = 3.29ISPTVGTPAATSSIWPIMPDD108 pKa = 2.94AKK110 pKa = 10.77GAGGGSFSADD120 pKa = 2.73VRR122 pKa = 11.84NGLEE126 pKa = 4.46VIGSNPPPGGYY137 pKa = 9.51PIVGEE142 pKa = 4.88LDD144 pKa = 3.33LKK146 pKa = 11.22AYY148 pKa = 9.76IYY150 pKa = 10.06LPKK153 pKa = 10.07GARR156 pKa = 11.84VLITAFGPSVPDD168 pKa = 3.11SGANLTLEE176 pKa = 4.07QAEE179 pKa = 4.34LGGTIASEE187 pKa = 4.06NYY189 pKa = 9.56ALNVGAGATASYY201 pKa = 10.3KK202 pKa = 10.54GYY204 pKa = 9.99FRR206 pKa = 11.84EE207 pKa = 4.08FTIPKK212 pKa = 9.89SFFARR217 pKa = 11.84PLSLSINRR225 pKa = 11.84AWYY228 pKa = 9.83GFTLNITVMNKK239 pKa = 9.05VPIMNASTGNMTLTWSPTEE258 pKa = 3.7PSIPLMVPAIKK269 pKa = 10.26PPALDD274 pKa = 3.1ISAIPFSNTRR284 pKa = 11.84VTALAALFTNVTKK297 pKa = 10.83ALNKK301 pKa = 9.89EE302 pKa = 4.17GTVMAGRR309 pKa = 11.84FNPATLDD316 pKa = 3.86ALDD319 pKa = 4.49LTMADD324 pKa = 3.89YY325 pKa = 11.03SQLAPCEE332 pKa = 4.21KK333 pKa = 10.58YY334 pKa = 10.55FFGLEE339 pKa = 3.94KK340 pKa = 10.74GFYY343 pKa = 9.81TYY345 pKa = 10.61IPLQTDD351 pKa = 3.12VGEE354 pKa = 4.45FADD357 pKa = 4.91DD358 pKa = 3.28VWDD361 pKa = 4.31APQISVTGGVRR372 pKa = 11.84IPWLHH377 pKa = 6.97LGRR380 pKa = 11.84TALVNTFRR388 pKa = 11.84FDD390 pKa = 3.93DD391 pKa = 4.52PDD393 pKa = 5.34GGTSLAVNLDD403 pKa = 2.98WHH405 pKa = 5.8VEE407 pKa = 3.94YY408 pKa = 10.85RR409 pKa = 11.84NSSVLWPVAVSALSLEE425 pKa = 4.42EE426 pKa = 3.61AHH428 pKa = 7.32RR429 pKa = 11.84AQLVCLEE436 pKa = 4.56AGFFFDD442 pKa = 5.4NVDD445 pKa = 3.27HH446 pKa = 6.98KK447 pKa = 11.09FILSMVSKK455 pKa = 10.13GLGMVAPMLKK465 pKa = 9.97NRR467 pKa = 11.84APAIASAGMAARR479 pKa = 11.84GYY481 pKa = 8.97IRR483 pKa = 11.84SYY485 pKa = 10.63LGEE488 pKa = 4.23SSHH491 pKa = 6.89PKK493 pKa = 9.76PSSLQVATAPRR504 pKa = 11.84VKK506 pKa = 10.21VRR508 pKa = 11.84HH509 pKa = 6.0PKK511 pKa = 10.1DD512 pKa = 3.5KK513 pKa = 10.29KK514 pKa = 10.84AKK516 pKa = 9.29AAPRR520 pKa = 11.84KK521 pKa = 9.35PKK523 pKa = 10.1SDD525 pKa = 3.03KK526 pKa = 10.55KK527 pKa = 10.65KK528 pKa = 10.4KK529 pKa = 10.19KK530 pKa = 9.88KK531 pKa = 10.33

Molecular weight:
57.12 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1629

531

1098

814.5

90.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.699 ± 0.342

2.394 ± 0.95

4.604 ± 0.044

4.85 ± 0.758

4.911 ± 0.497

6.446 ± 0.565

1.78 ± 0.338

3.683 ± 0.434

4.174 ± 0.864

9.208 ± 0.01

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.885 ± 0.066

3.376 ± 0.203

7.612 ± 0.839

2.578 ± 0.654

6.2 ± 1.166

5.893 ± 1.243

6.507 ± 0.435

7.551 ± 0.303

2.087 ± 0.204

3.56 ± 0.089

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski