Streptococcus phage 5093

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5IUL2|C5IUL2_9CAUD Uncharacterized protein OS=Streptococcus phage 5093 OX=646413 GN=st5093phage_36 PE=4 SV=1
MM1 pKa = 7.52SVQQAIVNWFVNHH14 pKa = 6.82RR15 pKa = 11.84GKK17 pKa = 9.04LTYY20 pKa = 11.06SMYY23 pKa = 10.84GSRR26 pKa = 11.84NGSDD30 pKa = 2.98GTADD34 pKa = 3.38CSGSISQALKK44 pKa = 9.74EE45 pKa = 4.19AGIGIQGLPSTVTLGQQLANNGFYY69 pKa = 10.21RR70 pKa = 11.84VSINQDD76 pKa = 2.14WDD78 pKa = 3.55ALTGDD83 pKa = 3.9IVMMSWGADD92 pKa = 3.23MSQSGGAGGHH102 pKa = 5.81VGVMMDD108 pKa = 3.26SVNFISCDD116 pKa = 3.3YY117 pKa = 8.82STQGAVGQAINTYY130 pKa = 8.91PWNDD134 pKa = 3.08YY135 pKa = 10.13YY136 pKa = 11.52AANKK140 pKa = 8.32PAYY143 pKa = 9.06IEE145 pKa = 3.74VWRR148 pKa = 11.84YY149 pKa = 9.05FDD151 pKa = 5.56NAPQTNNLANTSLKK165 pKa = 9.82PQEE168 pKa = 3.97KK169 pKa = 9.92AYY171 pKa = 11.1YY172 pKa = 8.39EE173 pKa = 4.13ANEE176 pKa = 3.98VQFVNGIYY184 pKa = 9.85QIKK187 pKa = 9.96CDD189 pKa = 4.03YY190 pKa = 10.48LSPIGFNYY198 pKa = 9.61YY199 pKa = 10.94DD200 pKa = 3.49NGVPVDD206 pKa = 3.65MVNWVDD212 pKa = 4.64KK213 pKa = 10.87DD214 pKa = 3.93GNDD217 pKa = 4.47IPDD220 pKa = 4.25GDD222 pKa = 4.31SKK224 pKa = 11.53DD225 pKa = 4.12FKK227 pKa = 11.39AGMFFSFAGDD237 pKa = 3.45EE238 pKa = 4.32VNITDD243 pKa = 3.77TGEE246 pKa = 3.65GGYY249 pKa = 10.59YY250 pKa = 9.99GGYY253 pKa = 7.88YY254 pKa = 8.11WRR256 pKa = 11.84KK257 pKa = 9.47FEE259 pKa = 4.33FGQFGTVWLSCWDD272 pKa = 3.5KK273 pKa = 11.78DD274 pKa = 4.68DD275 pKa = 5.02LVNYY279 pKa = 7.38YY280 pKa = 8.26QQ281 pKa = 4.22

Molecular weight: 31.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5IUJ9|C5IUJ9_9CAUD Putative minor capsid protein OS=Streptococcus phage 5093 OX=646413 GN=st5093phage_23 PE=4 SV=1
MM1 pKa = 6.97GTTVSVKK8 pKa = 10.13VDD10 pKa = 2.99LHH12 pKa = 7.19GLEE15 pKa = 4.88KK16 pKa = 10.49KK17 pKa = 10.47CSPEE21 pKa = 3.35AVKK24 pKa = 10.43RR25 pKa = 11.84GKK27 pKa = 10.24VAMIGQMITDD37 pKa = 4.47MEE39 pKa = 4.37PFIPRR44 pKa = 11.84RR45 pKa = 11.84DD46 pKa = 3.71GTLSASGSPFSDD58 pKa = 4.77GIRR61 pKa = 11.84YY62 pKa = 7.2PGPYY66 pKa = 10.0ARR68 pKa = 11.84AQFYY72 pKa = 10.7GSSYY76 pKa = 10.12NKK78 pKa = 9.31NRR80 pKa = 11.84SFVFRR85 pKa = 11.84NYY87 pKa = 7.31TTTGTGKK94 pKa = 10.1RR95 pKa = 11.84WDD97 pKa = 3.59MKK99 pKa = 11.21ASAKK103 pKa = 10.21YY104 pKa = 8.51SKK106 pKa = 10.29QWGEE110 pKa = 3.73VALRR114 pKa = 11.84AMGVKK119 pKa = 10.0

Molecular weight: 13.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

10909

40

1528

227.3

25.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.59 ± 1.221

0.486 ± 0.087

5.903 ± 0.589

7.489 ± 0.879

4.079 ± 0.223

6.004 ± 0.665

1.338 ± 0.209

7.297 ± 0.387

8.36 ± 0.64

7.948 ± 0.383

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.034 ± 0.36

5.674 ± 0.318

2.613 ± 0.198

4.437 ± 0.307

4.052 ± 0.505

6.811 ± 0.599

6.169 ± 0.359

5.656 ± 0.287

1.036 ± 0.13

4.024 ± 0.504
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski