Serinicoccus hydrothermalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Ornithinimicrobiaceae; Serinicoccus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3300 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1NAZ1|A0A1B1NAZ1_9MICO N-acetylmuramoyl-L-alanine amidase OS=Serinicoccus hydrothermalis OX=1758689 GN=SGUI_1196 PE=3 SV=1
MM1 pKa = 7.12HH2 pKa = 6.79QPSARR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84TRR11 pKa = 11.84LLALAGAGAVIAPLALVAPSAWANPDD37 pKa = 3.16LTVNEE42 pKa = 4.7DD43 pKa = 2.74GTYY46 pKa = 10.44IVMLADD52 pKa = 3.7SSLAAYY58 pKa = 10.12DD59 pKa = 4.07GGVQGIPATKK69 pKa = 9.11PAEE72 pKa = 4.25GEE74 pKa = 4.19KK75 pKa = 10.88LSTTTAAAEE84 pKa = 4.59DD85 pKa = 3.73YY86 pKa = 8.93TAYY89 pKa = 11.08LDD91 pKa = 3.79RR92 pKa = 11.84QQNAVLDD99 pKa = 4.21SVGLDD104 pKa = 3.18RR105 pKa = 11.84ADD107 pKa = 3.5AVYY110 pKa = 9.22TYY112 pKa = 9.72STAFNGFTADD122 pKa = 3.48LTGSQVSALRR132 pKa = 11.84KK133 pKa = 8.91DD134 pKa = 3.78PKK136 pKa = 10.71VAAVWEE142 pKa = 4.24NEE144 pKa = 3.44VRR146 pKa = 11.84YY147 pKa = 8.16TDD149 pKa = 4.23TITTPDD155 pKa = 3.76YY156 pKa = 11.38LGLTGDD162 pKa = 3.78DD163 pKa = 4.36GVWNTQFGGDD173 pKa = 3.63ANAGSGIVVGIIDD186 pKa = 3.49TGFWPEE192 pKa = 4.06NPSFAALPGDD202 pKa = 4.35PEE204 pKa = 5.42PPADD208 pKa = 3.49WNGEE212 pKa = 4.25CVEE215 pKa = 4.84GDD217 pKa = 3.74SADD220 pKa = 3.63PADD223 pKa = 4.68NVTCNSKK230 pKa = 10.85VIGARR235 pKa = 11.84FYY237 pKa = 10.81PDD239 pKa = 3.25TNDD242 pKa = 2.93TSFDD246 pKa = 3.75FEE248 pKa = 4.87SPRR251 pKa = 11.84DD252 pKa = 4.0TNGHH256 pKa = 5.84GSHH259 pKa = 6.62VGGTSAGNVDD269 pKa = 3.8VPMEE273 pKa = 4.3INGVEE278 pKa = 4.02LGEE281 pKa = 4.37GSGMAPAAHH290 pKa = 6.54LAFYY294 pKa = 9.93KK295 pKa = 10.76ALWQTEE301 pKa = 4.16DD302 pKa = 3.76GNGSGTSAGLVAAIDD317 pKa = 3.86DD318 pKa = 4.13AVADD322 pKa = 4.22GVDD325 pKa = 3.67VINYY329 pKa = 8.3SVSGSSDD336 pKa = 3.52YY337 pKa = 11.53VVTADD342 pKa = 4.17EE343 pKa = 5.11LAFLDD348 pKa = 4.1AANAGVFVSTSAGNSGDD365 pKa = 3.76TVGEE369 pKa = 3.8SSVAHH374 pKa = 5.73NSPWTMTVAASTHH387 pKa = 6.34DD388 pKa = 3.59RR389 pKa = 11.84TNSAEE394 pKa = 3.99VEE396 pKa = 4.3LGGGAPVTRR405 pKa = 11.84ISGTNRR411 pKa = 11.84YY412 pKa = 7.67DD413 pKa = 3.11TAAQIAAAYY422 pKa = 8.52PEE424 pKa = 4.84GVDD427 pKa = 3.21TVYY430 pKa = 10.64IATGNQFADD439 pKa = 3.78ALSGAAAASQGLVPANGLDD458 pKa = 3.77QPAVAPDD465 pKa = 4.26GSPAPVLLTKK475 pKa = 10.35VDD477 pKa = 4.95EE478 pKa = 4.76IPSATMEE485 pKa = 4.38ALEE488 pKa = 5.81SIDD491 pKa = 3.56PDD493 pKa = 3.88NIVVLGGEE501 pKa = 4.41VAVSDD506 pKa = 4.59DD507 pKa = 4.02VEE509 pKa = 4.7DD510 pKa = 4.14ALGDD514 pKa = 3.73YY515 pKa = 8.54GTVTRR520 pKa = 11.84VAGANRR526 pKa = 11.84YY527 pKa = 6.69EE528 pKa = 4.3TAALVAEE535 pKa = 4.58QYY537 pKa = 11.53GEE539 pKa = 3.93VDD541 pKa = 3.17HH542 pKa = 7.02VYY544 pKa = 10.61VATGQDD550 pKa = 3.19EE551 pKa = 4.73AFPDD555 pKa = 3.94ALSGSALAGSEE566 pKa = 4.19GVPVLLTKK574 pKa = 10.65SDD576 pKa = 3.88MVPGAVSDD584 pKa = 3.96ALEE587 pKa = 4.21AMGDD591 pKa = 3.87PEE593 pKa = 4.19VHH595 pKa = 6.62VIGGSAAVSEE605 pKa = 5.23DD606 pKa = 3.26VFEE609 pKa = 5.02DD610 pKa = 3.31LGGSEE615 pKa = 4.52RR616 pKa = 11.84LSGTDD621 pKa = 2.83RR622 pKa = 11.84YY623 pKa = 8.48GTSVAVAEE631 pKa = 4.25QFGYY635 pKa = 10.81DD636 pKa = 3.81ADD638 pKa = 3.93NPAPVVHH645 pKa = 6.58TATGRR650 pKa = 11.84DD651 pKa = 3.82YY652 pKa = 11.33PDD654 pKa = 4.02ALAGSALAGFQGVPVVLSRR673 pKa = 11.84PDD675 pKa = 3.28GLPNVVRR682 pKa = 11.84DD683 pKa = 3.9GMVALAPEE691 pKa = 4.33SAVILGGEE699 pKa = 4.22GALNDD704 pKa = 3.92DD705 pKa = 3.52VRR707 pKa = 11.84TEE709 pKa = 3.88IEE711 pKa = 4.34ALFEE715 pKa = 5.15DD716 pKa = 5.04SSQTYY721 pKa = 9.54PGISSGMGVGPAPLVNSVDD740 pKa = 3.11IVAADD745 pKa = 3.54STVADD750 pKa = 4.97AEE752 pKa = 4.35LCLPGSLDD760 pKa = 3.66AAGAADD766 pKa = 4.43NIVICTRR773 pKa = 11.84GTNARR778 pKa = 11.84VEE780 pKa = 4.15KK781 pKa = 10.81SEE783 pKa = 4.17TVAEE787 pKa = 4.22AGGIGMVLANNNNEE801 pKa = 3.76EE802 pKa = 4.17SLNGDD807 pKa = 3.78FHH809 pKa = 7.15SVPTIHH815 pKa = 7.27VDD817 pKa = 3.12GTTGDD822 pKa = 3.79AIKK825 pKa = 10.2AYY827 pKa = 8.88EE828 pKa = 4.38ASDD831 pKa = 4.03PNPTATISATGEE843 pKa = 4.38GPEE846 pKa = 4.0LTYY849 pKa = 10.97PEE851 pKa = 4.14MAGFSSYY858 pKa = 11.08GPASAGGGDD867 pKa = 4.33LLKK870 pKa = 10.49PDD872 pKa = 3.24ITAPGVDD879 pKa = 3.98VIAAVSPAGNGGNNFDD895 pKa = 4.52SYY897 pKa = 11.67SGTSMSAPHH906 pKa = 6.56IAGLAALMMQDD917 pKa = 3.41NPEE920 pKa = 4.13WSPMAVKK927 pKa = 10.49SSMMTTADD935 pKa = 3.49PTNSEE940 pKa = 4.34GEE942 pKa = 4.29LINYY946 pKa = 8.95AGAPASPLHH955 pKa = 5.84YY956 pKa = 10.35GSGEE960 pKa = 4.16VEE962 pKa = 3.93PGAAYY967 pKa = 7.64GTPLVYY973 pKa = 10.74DD974 pKa = 3.77SDD976 pKa = 4.51VIDD979 pKa = 4.25WIGYY983 pKa = 8.52ACSIGQWQLIGGAADD998 pKa = 3.71CADD1001 pKa = 4.75LGEE1004 pKa = 5.07SDD1006 pKa = 5.08PSDD1009 pKa = 3.68LNYY1012 pKa = 10.47PSIAIGDD1019 pKa = 3.86LGGTQTITRR1028 pKa = 11.84TVTDD1032 pKa = 3.65ASGEE1036 pKa = 4.3GGTFTAEE1043 pKa = 3.78VEE1045 pKa = 4.24APPGIDD1051 pKa = 2.86AVVEE1055 pKa = 4.03PSTITVEE1062 pKa = 4.25PGGTATFEE1070 pKa = 4.42VTFTTTDD1077 pKa = 3.1AEE1079 pKa = 4.32LGEE1082 pKa = 4.33WTFGSLTWTGPGEE1095 pKa = 4.28DD1096 pKa = 3.71VRR1098 pKa = 11.84SPIAINPVAMGAPAEE1113 pKa = 4.07IRR1115 pKa = 11.84GEE1117 pKa = 4.11GTEE1120 pKa = 4.09GSEE1123 pKa = 4.33TFEE1126 pKa = 4.12VTPGFSGEE1134 pKa = 4.18LTSVVHH1140 pKa = 6.81GLMASDD1146 pKa = 3.74VTDD1149 pKa = 4.82LDD1151 pKa = 3.95VTSDD1155 pKa = 3.62GPAGGDD1161 pKa = 3.75GIADD1165 pKa = 3.36AVVPVTVPEE1174 pKa = 4.24GTSAIRR1180 pKa = 11.84VEE1182 pKa = 4.54VVEE1185 pKa = 5.32SEE1187 pKa = 4.07WTPDD1191 pKa = 3.75GLDD1194 pKa = 3.45LDD1196 pKa = 5.63LYY1198 pKa = 11.12LADD1201 pKa = 3.85SEE1203 pKa = 5.11GNVVGQSAAGGSDD1216 pKa = 3.23EE1217 pKa = 5.34SVTTVAPAPGDD1228 pKa = 3.52YY1229 pKa = 10.66LVAIDD1234 pKa = 4.46FWSGAEE1240 pKa = 4.21GEE1242 pKa = 4.66VASGPLHH1249 pKa = 6.96VFMPSADD1256 pKa = 3.98EE1257 pKa = 4.69GNMTVTPSPVAVTAGVPLDD1276 pKa = 4.18LTAAWTGLDD1285 pKa = 3.01AATRR1289 pKa = 11.84YY1290 pKa = 10.15LGVIGYY1296 pKa = 7.48STGGEE1301 pKa = 4.08EE1302 pKa = 4.29VASTLVSVNTPP1313 pKa = 3.03

Molecular weight: 132.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1NCW4|A0A1B1NCW4_9MICO Foldase YidC OS=Serinicoccus hydrothermalis OX=1758689 GN=SGUI_1876 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.65GRR40 pKa = 11.84SALSAA45 pKa = 3.8

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3300

0

3300

1072852

37

2118

325.1

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.837 ± 0.058

0.645 ± 0.011

6.477 ± 0.042

6.351 ± 0.045

2.527 ± 0.026

9.671 ± 0.04

2.236 ± 0.024

3.068 ± 0.032

1.419 ± 0.029

10.583 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.907 ± 0.019

1.427 ± 0.025

5.728 ± 0.035

3.11 ± 0.024

7.847 ± 0.053

5.11 ± 0.031

6.058 ± 0.033

9.548 ± 0.04

1.606 ± 0.022

1.843 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski