Scheffersomyces segobiensis virus L

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Totivirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|M4MEM7|M4MEM7_9VIRU Isoform of M4MG99 RNA-directed RNA polymerase (Fragment) OS=Scheffersomyces segobiensis virus L OX=1300323 GN=pol PE=3 SV=1
MM1 pKa = 7.85SYY3 pKa = 10.93QLFDD7 pKa = 3.66QLTGPLANVLPNFKK21 pKa = 10.55YY22 pKa = 9.93LAKK25 pKa = 9.13TVKK28 pKa = 10.32YY29 pKa = 10.04GIKK32 pKa = 10.05ADD34 pKa = 3.41VDD36 pKa = 3.31IGVTSEE42 pKa = 3.62FTNAYY47 pKa = 8.5KK48 pKa = 10.55RR49 pKa = 11.84RR50 pKa = 11.84GALFTNVLSPFGVFNAEE67 pKa = 4.27VVRR70 pKa = 11.84DD71 pKa = 3.8PPIFDD76 pKa = 5.18GINARR81 pKa = 11.84FFNDD85 pKa = 2.91YY86 pKa = 11.56GEE88 pKa = 4.14IDD90 pKa = 3.41EE91 pKa = 4.6RR92 pKa = 11.84AIAEE96 pKa = 4.23TLRR99 pKa = 11.84LKK101 pKa = 9.56GTYY104 pKa = 9.06RR105 pKa = 11.84PNVIAEE111 pKa = 4.65LAPTIASCNSEE122 pKa = 3.75NHH124 pKa = 5.34VSVLVNLLRR133 pKa = 11.84FSIIKK138 pKa = 10.46RR139 pKa = 11.84SDD141 pKa = 3.1PKK143 pKa = 11.03DD144 pKa = 3.64FKK146 pKa = 11.07FVNDD150 pKa = 4.16DD151 pKa = 3.41LFYY154 pKa = 11.14DD155 pKa = 4.35DD156 pKa = 4.02GHH158 pKa = 7.31LAVSYY163 pKa = 11.0KK164 pKa = 10.84NYY166 pKa = 10.59LKK168 pKa = 10.82RR169 pKa = 11.84EE170 pKa = 4.09FNEE173 pKa = 3.52EE174 pKa = 3.6LVFIFRR180 pKa = 11.84EE181 pKa = 5.32GIQIEE186 pKa = 4.4RR187 pKa = 11.84FSEE190 pKa = 4.3STVVGDD196 pKa = 6.22DD197 pKa = 4.42FYY199 pKa = 11.75CPNIEE204 pKa = 4.02NLSARR209 pKa = 11.84EE210 pKa = 3.84FNILLTLLGSIKK222 pKa = 10.14CDD224 pKa = 3.37YY225 pKa = 10.06PLRR228 pKa = 11.84LAFSTPRR235 pKa = 11.84LVDD238 pKa = 3.54KK239 pKa = 11.01LVLPLTTKK247 pKa = 9.58HH248 pKa = 5.54ASYY251 pKa = 11.2YY252 pKa = 10.38EE253 pKa = 4.05VVNDD257 pKa = 3.82FTANEE262 pKa = 3.73VDD264 pKa = 3.68QIIRR268 pKa = 11.84KK269 pKa = 9.48YY270 pKa = 9.76IFANRR275 pKa = 11.84VEE277 pKa = 4.39TAFDD281 pKa = 3.65LAYY284 pKa = 10.49LIVVTAMFAPLPRR297 pKa = 11.84AIEE300 pKa = 4.1ANGWVSPVNLIKK312 pKa = 10.77LPNVGSVRR320 pKa = 11.84GLMPEE325 pKa = 3.91MTGGQPLSKK334 pKa = 10.32SPQKK338 pKa = 11.1ALTWLNYY345 pKa = 8.51KK346 pKa = 10.07QSPGRR351 pKa = 11.84IAIHH355 pKa = 7.13AIAACEE361 pKa = 3.6AYY363 pKa = 8.82YY364 pKa = 10.45TGFFEE369 pKa = 5.9VLTASPNGIEE379 pKa = 4.42DD380 pKa = 3.98TLTAIGVSSSVTATPYY396 pKa = 11.59KK397 pKa = 9.74MFCEE401 pKa = 4.08SAAYY405 pKa = 10.19RR406 pKa = 11.84FGKK409 pKa = 10.35EE410 pKa = 4.46FDD412 pKa = 5.32LIWLTNAGVDD422 pKa = 4.14CYY424 pKa = 11.54SHH426 pKa = 7.26LLATEE431 pKa = 4.39PAALSILAKK440 pKa = 10.72VVDD443 pKa = 4.39TEE445 pKa = 4.4LEE447 pKa = 4.4GYY449 pKa = 9.94DD450 pKa = 3.96VYY452 pKa = 9.28TTSAFGEE459 pKa = 4.46DD460 pKa = 3.59TVHH463 pKa = 7.31VITKK467 pKa = 7.57EE468 pKa = 3.95VKK470 pKa = 8.6PALFPVLSMGINDD483 pKa = 4.24DD484 pKa = 4.18RR485 pKa = 11.84YY486 pKa = 9.19FANSLEE492 pKa = 4.24YY493 pKa = 10.7SATLYY498 pKa = 10.83ADD500 pKa = 3.19HH501 pKa = 7.12SEE503 pKa = 4.28GVLATTEE510 pKa = 3.99SDD512 pKa = 3.68HH513 pKa = 6.18MNKK516 pKa = 9.98ALSILRR522 pKa = 11.84IGGYY526 pKa = 9.84DD527 pKa = 3.0AVMTDD532 pKa = 3.38RR533 pKa = 11.84LSGLSYY539 pKa = 10.92RR540 pKa = 11.84NWAANSNGQVLPMLPPKK557 pKa = 10.67LKK559 pKa = 10.67GKK561 pKa = 7.74STYY564 pKa = 10.36VIPFNTIVKK573 pKa = 10.06RR574 pKa = 11.84KK575 pKa = 8.63KK576 pKa = 9.55SWMEE580 pKa = 3.76LGSLTDD586 pKa = 3.83SVTMTAKK593 pKa = 10.26INLRR597 pKa = 11.84GYY599 pKa = 11.24VIMTNGKK606 pKa = 9.09LVSTYY611 pKa = 10.29LPKK614 pKa = 10.47YY615 pKa = 7.52QTINVYY621 pKa = 10.03PKK623 pKa = 10.42SLNKK627 pKa = 10.36EE628 pKa = 3.7EE629 pKa = 4.11MVAVEE634 pKa = 4.74MKK636 pKa = 10.44TSATTIKK643 pKa = 10.23YY644 pKa = 9.64RR645 pKa = 11.84YY646 pKa = 9.88SGFLLAASQVEE657 pKa = 4.3APPARR662 pKa = 11.84LQLLSTDD669 pKa = 3.59ILGSQPIEE677 pKa = 4.16TSSEE681 pKa = 3.7EE682 pKa = 3.93AGAVGDD688 pKa = 4.16DD689 pKa = 4.19LEE691 pKa = 4.59SVEE694 pKa = 4.41

Molecular weight:
77.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|M4MEM7|M4MEM7_9VIRU Isoform of M4MG99 RNA-directed RNA polymerase (Fragment) OS=Scheffersomyces segobiensis virus L OX=1300323 GN=pol PE=3 SV=1
II1 pKa = 7.25PVFGFFVGCEE11 pKa = 4.08SSGSTTCPFTIIVYY25 pKa = 6.79GHH27 pKa = 6.84PWVAAHH33 pKa = 6.91RR34 pKa = 11.84NIEE37 pKa = 3.98RR38 pKa = 11.84RR39 pKa = 11.84GWCSGRR45 pKa = 11.84RR46 pKa = 11.84LGEE49 pKa = 3.86CRR51 pKa = 11.84MSGLNYY57 pKa = 9.86HH58 pKa = 6.43NPVPLYY64 pKa = 10.4VDD66 pKa = 3.53EE67 pKa = 5.03NMAGTVPEE75 pKa = 3.94KK76 pKa = 10.46SRR78 pKa = 11.84YY79 pKa = 8.85VLVHH83 pKa = 5.76VSNDD87 pKa = 3.3DD88 pKa = 3.91VPVHH92 pKa = 6.36NIDD95 pKa = 3.53YY96 pKa = 10.72PMLIPYY102 pKa = 9.62CGEE105 pKa = 3.63YY106 pKa = 10.39TIGKK110 pKa = 9.01RR111 pKa = 11.84IKK113 pKa = 10.16RR114 pKa = 11.84RR115 pKa = 11.84NIVCWYY121 pKa = 10.0AKK123 pKa = 10.51LDD125 pKa = 4.25FISIYY130 pKa = 8.29TPKK133 pKa = 10.57LMSIVGGYY141 pKa = 9.64ISGNIRR147 pKa = 11.84SGMTVEE153 pKa = 4.02NHH155 pKa = 6.3RR156 pKa = 11.84DD157 pKa = 3.23FYY159 pKa = 11.27MEE161 pKa = 3.84FDD163 pKa = 3.22KK164 pKa = 11.69VIFNKK169 pKa = 8.5VTSDD173 pKa = 3.88EE174 pKa = 3.91IRR176 pKa = 11.84EE177 pKa = 4.02LNEE180 pKa = 3.65YY181 pKa = 9.64VRR183 pKa = 11.84SLDD186 pKa = 3.39VTKK189 pKa = 9.86ITGDD193 pKa = 2.74HH194 pKa = 6.36HH195 pKa = 7.13LFVNRR200 pKa = 11.84QEE202 pKa = 4.07VDD204 pKa = 3.36TLLEE208 pKa = 3.79EE209 pKa = 4.44AMNLGKK215 pKa = 11.01YY216 pKa = 9.07NDD218 pKa = 3.77RR219 pKa = 11.84LAALMVLKK227 pKa = 10.66DD228 pKa = 3.34VASRR232 pKa = 11.84IGIDD236 pKa = 3.31NRR238 pKa = 11.84VSFITHH244 pKa = 6.54LVYY247 pKa = 10.6ILIAPPHH254 pKa = 6.24AFVHH258 pKa = 6.04LLSLIGEE265 pKa = 4.43STDD268 pKa = 3.46AEE270 pKa = 4.59DD271 pKa = 3.59YY272 pKa = 11.46ASILKK277 pKa = 10.27RR278 pKa = 11.84EE279 pKa = 4.08SGAAKK284 pKa = 10.0QLQTVFRR291 pKa = 11.84SDD293 pKa = 3.26LASLYY298 pKa = 10.04EE299 pKa = 4.17LNVLVNRR306 pKa = 11.84VDD308 pKa = 5.51SVVDD312 pKa = 3.34WNKK315 pKa = 10.87EE316 pKa = 3.14IDD318 pKa = 3.12HH319 pKa = 6.17RR320 pKa = 11.84TNVKK324 pKa = 10.16AVGISTEE331 pKa = 4.09DD332 pKa = 3.39VYY334 pKa = 11.4KK335 pKa = 10.69ISTEE339 pKa = 3.69IFKK342 pKa = 10.92DD343 pKa = 3.52AVRR346 pKa = 11.84EE347 pKa = 3.92GRR349 pKa = 11.84YY350 pKa = 7.69PQRR353 pKa = 11.84LEE355 pKa = 3.83WQDD358 pKa = 3.05YY359 pKa = 7.84WVQRR363 pKa = 11.84WSAMPNGSVISQYY376 pKa = 11.52DD377 pKa = 3.28EE378 pKa = 5.6DD379 pKa = 3.94IAIKK383 pKa = 10.52KK384 pKa = 8.79LLPFSARR391 pKa = 11.84VKK393 pKa = 10.2SAWFAAKK400 pKa = 9.63TEE402 pKa = 4.34SNYY405 pKa = 11.01DD406 pKa = 3.27YY407 pKa = 10.34WLKK410 pKa = 9.87RR411 pKa = 11.84TPQIYY416 pKa = 10.24ASTSTKK422 pKa = 10.45YY423 pKa = 9.85EE424 pKa = 3.76WGKK427 pKa = 10.21VRR429 pKa = 11.84ALYY432 pKa = 10.68GCDD435 pKa = 2.86VTSFLHH441 pKa = 6.88SDD443 pKa = 3.8FGMTNCEE450 pKa = 4.99DD451 pKa = 3.48ILPSYY456 pKa = 10.42FPVGRR461 pKa = 11.84KK462 pKa = 9.44ANEE465 pKa = 3.86KK466 pKa = 9.51YY467 pKa = 10.31VRR469 pKa = 11.84EE470 pKa = 4.27SIEE473 pKa = 4.04KK474 pKa = 9.79FRR476 pKa = 11.84EE477 pKa = 4.08TVPVCFDD484 pKa = 3.45YY485 pKa = 11.46DD486 pKa = 3.86DD487 pKa = 5.49FNSQHH492 pKa = 5.86STSSMRR498 pKa = 11.84TVLQAWLDD506 pKa = 3.81VNQKK510 pKa = 10.18YY511 pKa = 7.77LTEE514 pKa = 4.04EE515 pKa = 3.91QVNSVEE521 pKa = 4.13WTRR524 pKa = 11.84DD525 pKa = 3.41SLDD528 pKa = 3.88SILARR533 pKa = 11.84FNSSGDD539 pKa = 3.8TISLSGTLMSGWRR552 pKa = 11.84LTSFMNTVLNRR563 pKa = 11.84VYY565 pKa = 10.48LVKK568 pKa = 11.04ANLFKK573 pKa = 10.91HH574 pKa = 6.23LVYY577 pKa = 10.71ALHH580 pKa = 6.7NGDD583 pKa = 4.85DD584 pKa = 3.93MFGGAVNMAKK594 pKa = 10.15AVAIIRR600 pKa = 11.84DD601 pKa = 3.53AKK603 pKa = 10.78AIGVRR608 pKa = 11.84AQVSKK613 pKa = 10.17TNLGTIGEE621 pKa = 4.4FLRR624 pKa = 11.84VDD626 pKa = 3.56TRR628 pKa = 11.84AIDD631 pKa = 3.66PTNAQYY637 pKa = 11.36LSRR640 pKa = 11.84SVATLVHH647 pKa = 5.98GRR649 pKa = 11.84VEE651 pKa = 4.35SSAPNDD657 pKa = 3.29LRR659 pKa = 11.84EE660 pKa = 4.24LVSAIITRR668 pKa = 11.84SEE670 pKa = 3.91EE671 pKa = 4.1VISRR675 pKa = 11.84GGDD678 pKa = 3.03FRR680 pKa = 11.84LINKK684 pKa = 7.75LTQKK688 pKa = 9.4TLDD691 pKa = 3.26FAARR695 pKa = 11.84LFNVDD700 pKa = 2.99NTIIDD705 pKa = 3.92TMLSNHH711 pKa = 6.32PVQGGMNKK719 pKa = 9.88DD720 pKa = 3.46APVHH724 pKa = 5.48LWRR727 pKa = 11.84IEE729 pKa = 3.67RR730 pKa = 11.84RR731 pKa = 11.84TEE733 pKa = 3.54PFEE736 pKa = 5.75RR737 pKa = 11.84EE738 pKa = 3.8TLLARR743 pKa = 11.84YY744 pKa = 9.62SLIAPGLFNYY754 pKa = 9.67VDD756 pKa = 3.45RR757 pKa = 11.84VKK759 pKa = 10.86EE760 pKa = 3.8QFGVSEE766 pKa = 3.97NLIDD770 pKa = 5.33KK771 pKa = 11.01EE772 pKa = 4.09EE773 pKa = 4.0LLYY776 pKa = 10.78RR777 pKa = 11.84AGVSLEE783 pKa = 3.71KK784 pKa = 10.84SRR786 pKa = 11.84VYY788 pKa = 10.82YY789 pKa = 10.23EE790 pKa = 4.35LVIEE794 pKa = 4.57TTLGMDD800 pKa = 4.31IYY802 pKa = 10.68RR803 pKa = 11.84ALHH806 pKa = 5.78GVWKK810 pKa = 10.42SPGYY814 pKa = 9.15VAPIAKK820 pKa = 9.37IRR822 pKa = 11.84SLGLIPAKK830 pKa = 9.05EE831 pKa = 3.9LRR833 pKa = 11.84NLNSVPAHH841 pKa = 7.24LIRR844 pKa = 11.84TARR847 pKa = 11.84NPILMMGTIFF857 pKa = 4.87

Molecular weight:
97.56 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

1

3

3051

694

1500

1017.0

114.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.211 ± 0.445

0.983 ± 0.039

5.539 ± 0.13

5.965 ± 0.076

4.326 ± 0.211

5.637 ± 0.029

1.901 ± 0.262

6.391 ± 0.173

5.179 ± 0.148

9.407 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.068

5.375 ± 0.056

4.064 ± 0.284

1.803 ± 0.065

5.867 ± 0.593

7.407 ± 0.064

6.227 ± 0.265

8.325 ± 0.195

1.311 ± 0.208

4.851 ± 0.11

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski