Changjiang tombus-like virus 7

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFY4|A0A1L3KFY4_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 7 OX=1922821 PE=4 SV=1
MM1 pKa = 7.14QWITANAFPCPPPPGLISSIFGEE24 pKa = 4.57VTITTYY30 pKa = 11.24GGTKK34 pKa = 9.91RR35 pKa = 11.84VWDD38 pKa = 3.45SRR40 pKa = 11.84KK41 pKa = 8.68IRR43 pKa = 11.84KK44 pKa = 7.09WAYY47 pKa = 9.15GAGAAASAALSLVALTAAGCGTSARR72 pKa = 11.84SLALRR77 pKa = 11.84TGAALLGVQAGAWLRR92 pKa = 11.84LHH94 pKa = 6.8YY95 pKa = 10.3DD96 pKa = 3.12AASTVPEE103 pKa = 4.01IDD105 pKa = 3.77EE106 pKa = 4.9LLAHH110 pKa = 6.62CADD113 pKa = 3.76IEE115 pKa = 4.32EE116 pKa = 4.44VTAVEE121 pKa = 4.49GKK123 pKa = 9.12PASEE127 pKa = 3.7AAEE130 pKa = 4.14ATKK133 pKa = 9.43TAANIVRR140 pKa = 11.84NWADD144 pKa = 2.81KK145 pKa = 10.78AYY147 pKa = 9.98AHH149 pKa = 6.85FGRR152 pKa = 11.84RR153 pKa = 11.84LEE155 pKa = 4.11DD156 pKa = 3.17TKK158 pKa = 11.55ADD160 pKa = 4.07RR161 pKa = 11.84DD162 pKa = 3.88ALGFWLARR170 pKa = 11.84QMKK173 pKa = 8.7EE174 pKa = 3.45GSVRR178 pKa = 11.84DD179 pKa = 3.67RR180 pKa = 11.84DD181 pKa = 3.45IAQYY185 pKa = 10.92LPLIVEE191 pKa = 4.36VCLLPSFAEE200 pKa = 4.03LTAQEE205 pKa = 4.16MRR207 pKa = 11.84RR208 pKa = 11.84SRR210 pKa = 11.84VANILRR216 pKa = 11.84RR217 pKa = 11.84EE218 pKa = 4.05CHH220 pKa = 5.72PPKK223 pKa = 10.76VV224 pKa = 3.56

Molecular weight: 24.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG41|A0A1L3KG41_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 7 OX=1922821 PE=4 SV=1
MM1 pKa = 7.57ARR3 pKa = 11.84NKK5 pKa = 8.73ITNKK9 pKa = 10.35SPMTARR15 pKa = 11.84NPGGNNRR22 pKa = 11.84GPRR25 pKa = 11.84KK26 pKa = 9.6RR27 pKa = 11.84AGPKK31 pKa = 9.33PKK33 pKa = 9.9RR34 pKa = 11.84ANARR38 pKa = 11.84IGARR42 pKa = 11.84ALDD45 pKa = 3.89ASAAAYY51 pKa = 9.82ARR53 pKa = 11.84LLADD57 pKa = 3.36PCGAPLVHH65 pKa = 6.42PTYY68 pKa = 10.69SGSEE72 pKa = 3.62GGYY75 pKa = 10.23LIRR78 pKa = 11.84AEE80 pKa = 4.26SFVTPGVGATEE91 pKa = 3.9NAGYY95 pKa = 9.82FSWVPGLIGNAAGIQVGAAAGAGTSVAANNFGQNAPGFNFLRR137 pKa = 11.84DD138 pKa = 3.49TASGARR144 pKa = 11.84CVAACLKK151 pKa = 10.36VSYY154 pKa = 9.85PGTEE158 pKa = 3.55AGRR161 pKa = 11.84AGRR164 pKa = 11.84VHH166 pKa = 7.47FGQTSGGSIIQGVGYY181 pKa = 8.12TADD184 pKa = 3.47NVAVLLPHH192 pKa = 5.57YY193 pKa = 8.75TRR195 pKa = 11.84TPAQEE200 pKa = 4.86FEE202 pKa = 5.13LVWKK206 pKa = 10.16PNDD209 pKa = 3.46ADD211 pKa = 3.58QLFRR215 pKa = 11.84DD216 pKa = 4.58PSALTSEE223 pKa = 4.44EE224 pKa = 4.32SKK226 pKa = 10.67SARR229 pKa = 11.84AALTVAWAGLPVATGLTFRR248 pKa = 11.84LTAVYY253 pKa = 9.18EE254 pKa = 4.1WQPASADD261 pKa = 3.73GLSVPNMSRR270 pKa = 11.84SPSEE274 pKa = 3.74NTLDD278 pKa = 3.59NVINFLTRR286 pKa = 11.84QGFTFVRR293 pKa = 11.84GMAMAGTSGLISGISNHH310 pKa = 6.13FGIMSAGVNTRR321 pKa = 11.84RR322 pKa = 11.84LAMM325 pKa = 4.55

Molecular weight: 33.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1035

224

486

345.0

37.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.754 ± 2.208

2.222 ± 0.552

3.865 ± 0.442

5.024 ± 0.793

4.155 ± 0.671

9.372 ± 1.177

2.415 ± 0.609

3.865 ± 0.342

3.478 ± 0.409

7.44 ± 0.616

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.609 ± 0.524

4.058 ± 0.96

5.7 ± 0.271

2.609 ± 0.159

7.633 ± 0.23

6.28 ± 0.469

5.411 ± 0.723

6.763 ± 0.477

1.643 ± 0.333

2.705 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski