Frankia inefficax (strain DSM 45817 / CECT 9037 / EuI1c)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7079 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3J9I4|E3J9I4_FRAIE Short-chain dehydrogenase/reductase SDR OS=Frankia inefficax (strain DSM 45817 / CECT 9037 / EuI1c) OX=298654 GN=FraEuI1c_5360 PE=4 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84HH4 pKa = 5.2ITIDD8 pKa = 3.33RR9 pKa = 11.84DD10 pKa = 3.19LCMGSGQCLIYY21 pKa = 10.78APNTFDD27 pKa = 6.12LDD29 pKa = 3.67DD30 pKa = 4.61DD31 pKa = 5.59AIATVVDD38 pKa = 3.89PDD40 pKa = 3.9GDD42 pKa = 3.86SDD44 pKa = 5.03AEE46 pKa = 4.16LASAVTGCPTQAISVARR63 pKa = 11.84DD64 pKa = 2.98

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3J063|E3J063_FRAIE Uncharacterized protein OS=Frankia inefficax (strain DSM 45817 / CECT 9037 / EuI1c) OX=298654 GN=FraEuI1c_2307 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 8.58THH17 pKa = 5.46GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIISGRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.84GRR40 pKa = 11.84AEE42 pKa = 3.84LSAA45 pKa = 4.93

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7079

0

7079

2517300

30

8463

355.6

37.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.714 ± 0.048

0.735 ± 0.008

6.106 ± 0.023

4.856 ± 0.03

2.72 ± 0.016

9.736 ± 0.029

2.066 ± 0.015

3.297 ± 0.02

1.509 ± 0.017

10.291 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.553 ± 0.011

1.701 ± 0.014

6.665 ± 0.033

2.619 ± 0.014

8.148 ± 0.039

5.08 ± 0.025

6.167 ± 0.044

8.657 ± 0.033

1.48 ± 0.013

1.901 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski