Mycolicibacterium fallax (Mycobacterium fallax)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3787 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1R7U7|A0A1X1R7U7_MYCFA Uncharacterized protein OS=Mycolicibacterium fallax OX=1793 GN=AWC04_15025 PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 5.11NDD4 pKa = 4.06IIALIDD10 pKa = 3.35QALDD14 pKa = 3.19EE15 pKa = 4.94GEE17 pKa = 4.69PYY19 pKa = 10.8SPADD23 pKa = 5.72DD24 pKa = 4.31PYY26 pKa = 10.28SWNDD30 pKa = 3.04AAVWTSTGDD39 pKa = 3.39HH40 pKa = 6.47QDD42 pKa = 3.13RR43 pKa = 11.84DD44 pKa = 3.65DD45 pKa = 5.06DD46 pKa = 4.01EE47 pKa = 7.21RR48 pKa = 11.84DD49 pKa = 3.82DD50 pKa = 4.36EE51 pKa = 4.65VDD53 pKa = 3.06PLRR56 pKa = 11.84YY57 pKa = 9.54VDD59 pKa = 4.8NPFAGTYY66 pKa = 8.95YY67 pKa = 10.2QDD69 pKa = 3.98PSDD72 pKa = 4.61PPWLVTPVGHH82 pKa = 5.47QHH84 pKa = 6.9IYY86 pKa = 11.09DD87 pKa = 3.83HH88 pKa = 7.61DD89 pKa = 4.17EE90 pKa = 4.0PAPAVWEE97 pKa = 4.49VSRR100 pKa = 11.84QPPGCYY106 pKa = 9.17SDD108 pKa = 5.01EE109 pKa = 4.35PQAGQTVVLSATLSDD124 pKa = 4.14GLRR127 pKa = 11.84TCYY130 pKa = 8.76FTYY133 pKa = 10.93VHH135 pKa = 6.97GEE137 pKa = 4.08DD138 pKa = 4.08PQHH141 pKa = 6.8GEE143 pKa = 3.57WDD145 pKa = 3.44GDD147 pKa = 3.5ADD149 pKa = 3.5MLEE152 pKa = 4.2AVRR155 pKa = 11.84ILGALDD161 pKa = 4.35PPDD164 pKa = 4.74DD165 pKa = 4.66PLRR168 pKa = 11.84MIAGALARR176 pKa = 11.84VGWWGTVSDD185 pKa = 4.81SVWQVLAGDD194 pKa = 4.95DD195 pKa = 3.88YY196 pKa = 12.2

Molecular weight: 21.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1RG22|A0A1X1RG22_MYCFA MarR family transcriptional regulator OS=Mycolicibacterium fallax OX=1793 GN=AWC04_07295 PE=4 SV=1
MM1 pKa = 7.03QRR3 pKa = 11.84RR4 pKa = 11.84NRR6 pKa = 11.84RR7 pKa = 11.84AGVEE11 pKa = 4.03DD12 pKa = 3.19RR13 pKa = 11.84WRR15 pKa = 11.84RR16 pKa = 11.84SDD18 pKa = 3.37GSPTARR24 pKa = 11.84SGMGRR29 pKa = 11.84RR30 pKa = 11.84WLARR34 pKa = 11.84YY35 pKa = 9.79VDD37 pKa = 3.94DD38 pKa = 5.11QGGEE42 pKa = 4.0NTRR45 pKa = 11.84SFDD48 pKa = 4.05RR49 pKa = 11.84KK50 pKa = 9.99VDD52 pKa = 3.37AQAFLNEE59 pKa = 3.63ITAAQTIGTYY69 pKa = 9.19VAPKK73 pKa = 9.75AGRR76 pKa = 11.84ITVRR80 pKa = 11.84EE81 pKa = 4.06LHH83 pKa = 6.31GKK85 pKa = 8.01WLGTQGHH92 pKa = 6.39LKK94 pKa = 8.21EE95 pKa = 4.48TTVATRR101 pKa = 11.84AFAWSGYY108 pKa = 9.27VEE110 pKa = 4.29GRR112 pKa = 11.84WAAVAVADD120 pKa = 4.31VQSSDD125 pKa = 2.39IRR127 pKa = 11.84AWVQQLAAGGAKK139 pKa = 9.81PATIEE144 pKa = 3.92NALSVLRR151 pKa = 11.84QILEE155 pKa = 3.92MAVDD159 pKa = 3.92DD160 pKa = 5.0RR161 pKa = 11.84RR162 pKa = 11.84IPRR165 pKa = 11.84NPCTGVKK172 pKa = 9.7SPRR175 pKa = 11.84RR176 pKa = 11.84QHH178 pKa = 6.43RR179 pKa = 11.84ARR181 pKa = 11.84GYY183 pKa = 8.21LTHH186 pKa = 6.17QQVEE190 pKa = 4.16LLARR194 pKa = 11.84EE195 pKa = 4.12VGEE198 pKa = 4.04YY199 pKa = 10.71AVVVRR204 pKa = 11.84FLAYY208 pKa = 9.27TGLRR212 pKa = 11.84WGEE215 pKa = 3.76MAALRR220 pKa = 11.84VEE222 pKa = 4.32SFDD225 pKa = 3.24MLRR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84VNIRR234 pKa = 11.84EE235 pKa = 3.81AVAEE239 pKa = 4.26VKK241 pKa = 10.74GRR243 pKa = 11.84VVWSSPKK250 pKa = 8.22SHH252 pKa = 6.39EE253 pKa = 4.18RR254 pKa = 11.84RR255 pKa = 11.84SVPFPAFLADD265 pKa = 3.7PLAAIMIGKK274 pKa = 9.37RR275 pKa = 11.84RR276 pKa = 11.84DD277 pKa = 3.61DD278 pKa = 4.18LVFTSPGGALLRR290 pKa = 11.84VSTWRR295 pKa = 11.84PRR297 pKa = 11.84VFNMAVQRR305 pKa = 11.84LQEE308 pKa = 4.53ADD310 pKa = 3.23PAYY313 pKa = 8.32PTVTPHH319 pKa = 7.59DD320 pKa = 4.26LRR322 pKa = 11.84HH323 pKa = 5.48TAASLSISAGANVKK337 pKa = 9.86AVQTMLGHH345 pKa = 6.81ASAVLTLDD353 pKa = 3.76TYY355 pKa = 12.12ADD357 pKa = 4.59LFPDD361 pKa = 4.06DD362 pKa = 4.89LEE364 pKa = 4.43QVSVALDD371 pKa = 3.57AARR374 pKa = 11.84MRR376 pKa = 11.84SLAATADD383 pKa = 3.49QLRR386 pKa = 11.84TGKK389 pKa = 10.35

Molecular weight: 43.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3787

0

3787

1219274

29

3072

322.0

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.042 ± 0.057

0.777 ± 0.009

6.321 ± 0.033

5.193 ± 0.029

2.886 ± 0.022

9.183 ± 0.042

2.063 ± 0.021

4.147 ± 0.026

2.017 ± 0.029

10.068 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.974 ± 0.017

2.122 ± 0.019

6.058 ± 0.04

2.851 ± 0.021

7.452 ± 0.041

4.91 ± 0.027

5.955 ± 0.027

8.42 ± 0.034

1.472 ± 0.017

2.089 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski