Macroptilium yellow mosaic virus-[Cuba]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Macroptilium yellow mosaic virus

Average proteome isoelectric point is 8.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q911W6|Q911W6_9GEMI Transcriptional activator protein OS=Macroptilium yellow mosaic virus-[Cuba] OX=223285 GN=AC2 PE=3 SV=1
MM1 pKa = 7.85PPPKK5 pKa = 9.88RR6 pKa = 11.84FRR8 pKa = 11.84VSARR12 pKa = 11.84NYY14 pKa = 8.8FLTYY18 pKa = 7.96PHH20 pKa = 7.14CSLSKK25 pKa = 11.01EE26 pKa = 4.1EE27 pKa = 5.45ALSQLQNISTPTNKK41 pKa = 9.86KK42 pKa = 9.95FIRR45 pKa = 11.84ICEE48 pKa = 4.04EE49 pKa = 3.65LHH51 pKa = 7.41EE52 pKa = 5.38DD53 pKa = 4.09GQPHH57 pKa = 6.47LHH59 pKa = 6.28VLIQFEE65 pKa = 4.8GKK67 pKa = 9.67FVCTNNRR74 pKa = 11.84LFDD77 pKa = 4.04LVSPSRR83 pKa = 11.84SAHH86 pKa = 5.01FHH88 pKa = 6.64PNVQGAKK95 pKa = 9.76SSSDD99 pKa = 3.17VKK101 pKa = 11.17SYY103 pKa = 11.15LDD105 pKa = 3.43KK106 pKa = 11.58DD107 pKa = 3.88GVTVEE112 pKa = 3.77WGEE115 pKa = 3.88FQVDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.15SYY134 pKa = 12.0AKK136 pKa = 10.46ALNASSVEE144 pKa = 3.9QALQILKK151 pKa = 10.35EE152 pKa = 4.01EE153 pKa = 4.1QPKK156 pKa = 11.06DD157 pKa = 3.62FLLQNHH163 pKa = 6.99NIRR166 pKa = 11.84SNLEE170 pKa = 3.88RR171 pKa = 11.84IFAKK175 pKa = 10.49APEE178 pKa = 4.14PWVPPFHH185 pKa = 7.0LSSLNNVPAEE195 pKa = 3.99MQAWVDD201 pKa = 3.76DD202 pKa = 3.99YY203 pKa = 11.47FGRR206 pKa = 11.84GAAARR211 pKa = 11.84PEE213 pKa = 4.08RR214 pKa = 11.84PISIIVEE221 pKa = 4.05GDD223 pKa = 3.08SRR225 pKa = 11.84TGKK228 pKa = 8.52TMWARR233 pKa = 11.84SLGPHH238 pKa = 6.57NYY240 pKa = 10.17LSGHH244 pKa = 6.91LDD246 pKa = 3.58FNSRR250 pKa = 11.84IYY252 pKa = 11.15SNEE255 pKa = 3.2VLYY258 pKa = 11.16NVIDD262 pKa = 5.13DD263 pKa = 4.36ISPNYY268 pKa = 10.3LKK270 pKa = 10.74LKK272 pKa = 9.13HH273 pKa = 5.7WKK275 pKa = 9.67EE276 pKa = 3.98LIGAQKK282 pKa = 10.57DD283 pKa = 3.42WQSNCKK289 pKa = 9.38YY290 pKa = 10.21GKK292 pKa = 9.18PVQIKK297 pKa = 10.19GGIPSIVLCNPGEE310 pKa = 4.16GASYY314 pKa = 11.29KK315 pKa = 10.87DD316 pKa = 3.72FLDD319 pKa = 3.94KK320 pKa = 11.28EE321 pKa = 4.54EE322 pKa = 4.68NRR324 pKa = 11.84ALHH327 pKa = 6.09NWTIHH332 pKa = 5.36NALFITLTSPLYY344 pKa = 10.63QSTPP348 pKa = 3.2

Molecular weight: 39.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q911W8|Q911W8_9GEMI Capsid protein OS=Macroptilium yellow mosaic virus-[Cuba] OX=223285 GN=AV1 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.65RR4 pKa = 11.84DD5 pKa = 3.82APWRR9 pKa = 11.84LMAGTSKK16 pKa = 9.07VTRR19 pKa = 11.84SGNFSPKK26 pKa = 10.07SGLGQGSNKK35 pKa = 9.31NAWVNRR41 pKa = 11.84PMYY44 pKa = 9.63RR45 pKa = 11.84KK46 pKa = 8.62PRR48 pKa = 11.84IYY50 pKa = 10.8RR51 pKa = 11.84MYY53 pKa = 10.38RR54 pKa = 11.84SSDD57 pKa = 3.5VPKK60 pKa = 10.71GCEE63 pKa = 4.37GPCKK67 pKa = 9.35VQSYY71 pKa = 6.94EE72 pKa = 3.74QRR74 pKa = 11.84HH75 pKa = 6.38DD76 pKa = 3.16ISHH79 pKa = 6.13VGKK82 pKa = 10.58VMCISDD88 pKa = 3.41ITRR91 pKa = 11.84GNGITHH97 pKa = 6.78RR98 pKa = 11.84VGKK101 pKa = 9.44RR102 pKa = 11.84FCVKK106 pKa = 9.92SVYY109 pKa = 10.16ILGKK113 pKa = 9.37IWMDD117 pKa = 3.39EE118 pKa = 4.0NIKK121 pKa = 10.61LKK123 pKa = 10.71NHH125 pKa = 5.43TNSVIFWLVRR135 pKa = 11.84DD136 pKa = 3.82RR137 pKa = 11.84RR138 pKa = 11.84PYY140 pKa = 8.05GTPMDD145 pKa = 4.6FGQVFNMFDD154 pKa = 3.99NEE156 pKa = 4.11PSTATVKK163 pKa = 10.85NDD165 pKa = 2.82LRR167 pKa = 11.84DD168 pKa = 3.48RR169 pKa = 11.84FQVMHH174 pKa = 7.06RR175 pKa = 11.84FHH177 pKa = 7.13SKK179 pKa = 7.91VTGGQYY185 pKa = 11.06ASNEE189 pKa = 3.84QALVRR194 pKa = 11.84RR195 pKa = 11.84FWKK198 pKa = 10.4VNNHH202 pKa = 4.85VVYY205 pKa = 10.66SHH207 pKa = 6.33QEE209 pKa = 3.33AGKK212 pKa = 10.53YY213 pKa = 8.4EE214 pKa = 4.09NHH216 pKa = 6.52TEE218 pKa = 3.99NALLLYY224 pKa = 7.29MACTHH229 pKa = 7.07ASNPVYY235 pKa = 9.86ATLKK239 pKa = 9.47IRR241 pKa = 11.84IYY243 pKa = 10.59FYY245 pKa = 11.38DD246 pKa = 4.2SITNN250 pKa = 3.6

Molecular weight: 28.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

861

129

348

215.3

24.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.11 ± 0.602

1.742 ± 0.351

4.762 ± 0.411

4.413 ± 0.791

4.878 ± 0.581

6.388 ± 0.469

4.762 ± 0.919

5.575 ± 0.414

5.923 ± 0.598

7.317 ± 1.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.858 ± 0.756

6.62 ± 0.468

5.575 ± 0.661

4.181 ± 0.345

6.62 ± 0.994

8.13 ± 1.036

4.53 ± 1.0

5.923 ± 1.015

1.742 ± 0.204

3.949 ± 0.632
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski