Pectobacterium phage Clickz_B3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Corkvirinae; Phimunavirus; unclassified Phimunavirus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FHC8|A0A3G8FHC8_9CAUD Uncharacterized protein OS=Pectobacterium phage Clickz_B3 OX=2489620 PE=4 SV=1
MM1 pKa = 7.22INKK4 pKa = 6.68VQRR7 pKa = 11.84YY8 pKa = 7.31EE9 pKa = 3.88LTEE12 pKa = 3.82MSFNPYY18 pKa = 9.73EE19 pKa = 4.48SYY21 pKa = 11.15RR22 pKa = 11.84CMEE25 pKa = 4.53EE26 pKa = 5.09DD27 pKa = 3.32EE28 pKa = 4.72QGDD31 pKa = 3.77FVRR34 pKa = 11.84FEE36 pKa = 5.54DD37 pKa = 4.04YY38 pKa = 11.02DD39 pKa = 3.98KK40 pKa = 11.37LAEE43 pKa = 4.96DD44 pKa = 4.27YY45 pKa = 8.54ATLLEE50 pKa = 5.23RR51 pKa = 11.84YY52 pKa = 9.41DD53 pKa = 5.01LDD55 pKa = 3.9VNPEE59 pKa = 3.72GKK61 pKa = 10.2

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FHA1|A0A3G8FHA1_9CAUD HNH homing endonuclease OS=Pectobacterium phage Clickz_B3 OX=2489620 PE=4 SV=1
MM1 pKa = 7.29FSKK4 pKa = 11.03GIYY7 pKa = 9.75FSSTTEE13 pKa = 3.84QEE15 pKa = 3.63KK16 pKa = 10.19QAALKK21 pKa = 10.08QSKK24 pKa = 7.98NTLCKK29 pKa = 10.27LLYY32 pKa = 9.59YY33 pKa = 10.24KK34 pKa = 10.61KK35 pKa = 9.91KK36 pKa = 10.57LSSALYY42 pKa = 9.76RR43 pKa = 11.84EE44 pKa = 4.08RR45 pKa = 11.84NEE47 pKa = 3.77RR48 pKa = 11.84KK49 pKa = 8.89IRR51 pKa = 11.84RR52 pKa = 11.84MRR54 pKa = 11.84II55 pKa = 3.16

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13623

40

1263

247.7

27.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.704 ± 0.415

1.167 ± 0.174

6.203 ± 0.258

5.219 ± 0.34

3.068 ± 0.126

7.715 ± 0.275

2.004 ± 0.201

4.426 ± 0.183

4.603 ± 0.333

8.721 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.192

4.397 ± 0.336

4.037 ± 0.22

4.859 ± 0.448

5.549 ± 0.301

6.408 ± 0.342

6.467 ± 0.385

7.465 ± 0.247

1.387 ± 0.131

3.861 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski