Actinotalea fermentans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia<

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3422 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A511YV90|A0A511YV90_9CELL NAD(P)-dependent oxidoreductase OS=Actinotalea fermentans OX=43671 GN=AFE02nite_08560 PE=4 SV=1
MM1 pKa = 7.42AAVFSNRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84ALAMTAGTAAVVVALAGCGGADD32 pKa = 4.62DD33 pKa = 5.29GGSGDD38 pKa = 3.46GGGAGGEE45 pKa = 4.16LVIGTTDD52 pKa = 3.87KK53 pKa = 10.49ITTIDD58 pKa = 3.49PAGSYY63 pKa = 11.17DD64 pKa = 3.54NGSFAVMNQVFPFLLNTPLGSPDD87 pKa = 3.76VEE89 pKa = 3.9PDD91 pKa = 3.01IAEE94 pKa = 4.25SAEE97 pKa = 4.09FTSPTEE103 pKa = 3.78YY104 pKa = 10.4TVVLKK109 pKa = 10.98EE110 pKa = 4.02GLTFANGNEE119 pKa = 4.2LTSSDD124 pKa = 3.59VKK126 pKa = 10.49FTFDD130 pKa = 2.57RR131 pKa = 11.84MQAIFEE137 pKa = 4.3SGADD141 pKa = 3.58GGNGPGSLLYY151 pKa = 10.6NLEE154 pKa = 4.14STEE157 pKa = 4.08ATDD160 pKa = 3.2EE161 pKa = 4.24LTVVFHH167 pKa = 6.83LKK169 pKa = 10.4SEE171 pKa = 4.12NDD173 pKa = 3.26QVFPQILSSPAGPIVDD189 pKa = 3.9EE190 pKa = 5.08DD191 pKa = 4.14VFAADD196 pKa = 5.06ALTPDD201 pKa = 3.81ADD203 pKa = 3.48IVAGNAFAGPYY214 pKa = 10.27VITSYY219 pKa = 11.45DD220 pKa = 3.76LNNLISYY227 pKa = 7.56EE228 pKa = 4.27ANPDD232 pKa = 3.55YY233 pKa = 10.88QGLLGAPRR241 pKa = 11.84NSAVNVKK248 pKa = 10.08YY249 pKa = 10.55YY250 pKa = 11.3AEE252 pKa = 4.27ASNLKK257 pKa = 10.42LDD259 pKa = 3.89VQEE262 pKa = 4.8GNVDD266 pKa = 3.16VAYY269 pKa = 10.41RR270 pKa = 11.84SLSATDD276 pKa = 3.71IEE278 pKa = 4.98DD279 pKa = 3.5LRR281 pKa = 11.84GDD283 pKa = 3.78EE284 pKa = 4.15NVEE287 pKa = 4.21VVDD290 pKa = 4.75GPGGEE295 pKa = 3.55IRR297 pKa = 11.84YY298 pKa = 9.34IVFNFDD304 pKa = 3.41TQPYY308 pKa = 9.09GATTAEE314 pKa = 3.98ADD316 pKa = 3.66EE317 pKa = 4.68AKK319 pKa = 10.75ALAVRR324 pKa = 11.84QAVAHH329 pKa = 6.5LIDD332 pKa = 3.83RR333 pKa = 11.84AEE335 pKa = 4.27LAEE338 pKa = 3.93QVYY341 pKa = 10.4KK342 pKa = 10.16GTYY345 pKa = 7.21TPLYY349 pKa = 10.19SFVPDD354 pKa = 3.66GLTGATEE361 pKa = 4.06ALKK364 pKa = 10.63DD365 pKa = 3.96LYY367 pKa = 11.27GDD369 pKa = 3.84GDD371 pKa = 4.05GGPDD375 pKa = 3.43ADD377 pKa = 3.77AAAAVLEE384 pKa = 4.49EE385 pKa = 4.74AGVTTPVQLSLQYY398 pKa = 11.43SNDD401 pKa = 3.17HH402 pKa = 6.35YY403 pKa = 11.31GPSSGDD409 pKa = 3.07EE410 pKa = 3.82YY411 pKa = 11.92ALIKK415 pKa = 10.69DD416 pKa = 3.71QLEE419 pKa = 4.2SSGLFTVDD427 pKa = 3.29LQTTEE432 pKa = 3.92WVQYY436 pKa = 10.29AQDD439 pKa = 3.85RR440 pKa = 11.84TSDD443 pKa = 3.69VYY445 pKa = 10.52PAYY448 pKa = 10.27QLGWFPDD455 pKa = 3.67YY456 pKa = 11.48SDD458 pKa = 4.11ADD460 pKa = 3.57NYY462 pKa = 8.91LTPFFLVEE470 pKa = 4.03NFLGNHH476 pKa = 5.49YY477 pKa = 10.88VNQEE481 pKa = 3.95VNDD484 pKa = 4.43MILEE488 pKa = 4.18QAVTTDD494 pKa = 2.83ADD496 pKa = 3.32ARR498 pKa = 11.84TALIEE503 pKa = 4.93DD504 pKa = 3.59IQAAVAQDD512 pKa = 4.35LSTVPYY518 pKa = 10.3LQGAQVAVVRR528 pKa = 11.84TGVTGAGDD536 pKa = 3.48TLDD539 pKa = 3.92ASFKK543 pKa = 10.38FRR545 pKa = 11.84YY546 pKa = 8.91GALAKK551 pKa = 10.5DD552 pKa = 3.61

Molecular weight: 58.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A511Z254|A0A511Z254_9CELL Uncharacterized protein OS=Actinotalea fermentans OX=43671 GN=AFE02nite_32100 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.88GRR40 pKa = 11.84EE41 pKa = 3.56NLSAA45 pKa = 4.59

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3422

0

3422

1139067

39

2802

332.9

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.222 ± 0.055

0.599 ± 0.01

6.273 ± 0.033

5.435 ± 0.042

2.443 ± 0.025

9.567 ± 0.039

2.01 ± 0.019

2.893 ± 0.032

1.252 ± 0.024

10.309 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.678 ± 0.015

1.424 ± 0.022

6.123 ± 0.035

2.531 ± 0.02

7.972 ± 0.048

4.566 ± 0.028

6.084 ± 0.04

10.261 ± 0.046

1.578 ± 0.019

1.776 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski