Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces pombe

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5151 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O13969|SYTM_SCHPO Probable threonine--tRNA ligase mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC24C9.09 PE=3 SV=2
MM1 pKa = 7.47LFKK4 pKa = 10.99SFALTLLFAAARR16 pKa = 11.84VQAASNCSSGPYY28 pKa = 9.69NISAQGTLDD37 pKa = 3.7EE38 pKa = 5.45LNSCTVLNGDD48 pKa = 5.31LYY50 pKa = 10.77ISDD53 pKa = 4.64AGNSGITTLTVNGIEE68 pKa = 4.3SVQGDD73 pKa = 4.11VVVSDD78 pKa = 3.78GQYY81 pKa = 9.1LTSLSFPSLKK91 pKa = 10.35NVSGAFNVNNMIRR104 pKa = 11.84MNNLATPEE112 pKa = 4.08LTSVGSLNLAVLPNLQEE129 pKa = 4.11LQFNAGLSDD138 pKa = 3.64SDD140 pKa = 4.27SVVIDD145 pKa = 3.74DD146 pKa = 4.67TQLQAIDD153 pKa = 5.12GISLDD158 pKa = 3.81SVTTFQVTNNRR169 pKa = 11.84YY170 pKa = 7.91IQEE173 pKa = 3.7ITMEE177 pKa = 4.38GLEE180 pKa = 4.27SAQNIQISANSKK192 pKa = 8.5GVSVNFSKK200 pKa = 11.0LSNVTTATFDD210 pKa = 4.08GISNVFIGNLKK221 pKa = 9.74SAAGNLYY228 pKa = 10.45FSNTTLDD235 pKa = 4.31NISVPYY241 pKa = 8.75LTEE244 pKa = 4.59IGQSFAVLYY253 pKa = 10.62SPEE256 pKa = 4.18LTSLNFPNLTTVGGGFVINDD276 pKa = 3.75TGLTSIDD283 pKa = 3.66GFPVISEE290 pKa = 3.92IGGGLVLLGNFSSIDD305 pKa = 3.67MPDD308 pKa = 3.28LSDD311 pKa = 3.45VKK313 pKa = 10.96GALTVEE319 pKa = 4.88TKK321 pKa = 9.4ATNFTCPWSNDD332 pKa = 3.08DD333 pKa = 5.11SVIKK337 pKa = 10.76GDD339 pKa = 5.22DD340 pKa = 3.7FTCQGSVATISATSSYY356 pKa = 10.67DD357 pKa = 3.35LSSTVSATSGSATSATGSATTTSYY381 pKa = 11.53SSDD384 pKa = 3.06SSASSSSSSSHH395 pKa = 5.98EE396 pKa = 3.83SSAASNGFTAGALVLGSLLVAALAMM421 pKa = 4.66

Molecular weight: 43.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0CT98|YM01_SCHPO UPF0494 membrane protein SPAC212.01c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC212.01c PE=3 SV=1
MRDKWRKKRVRRLKRKRRKMRARSK

Molecular weight: 3.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5138

13

5151

2391875

24

4924

464.4

52.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.224 ± 0.024

1.468 ± 0.013

5.339 ± 0.022

6.522 ± 0.034

4.616 ± 0.025

4.929 ± 0.03

2.26 ± 0.014

6.158 ± 0.025

6.433 ± 0.033

9.871 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.013

5.212 ± 0.02

4.713 ± 0.026

3.819 ± 0.023

4.867 ± 0.023

9.425 ± 0.058

5.506 ± 0.045

6.035 ± 0.022

1.119 ± 0.011

3.419 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski