Cronobacter phage vB_CskP_GAP227

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Cronosvirus; Cronobacter virus GAP227

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9RZY9|K9RZY9_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CskP_GAP227 OX=1264737 GN=GAP227_09 PE=4 SV=1
MM1 pKa = 6.83FTVFLSAFRR10 pKa = 11.84GEE12 pKa = 4.23RR13 pKa = 11.84TFEE16 pKa = 4.04QNVLKK21 pKa = 10.67SAEE24 pKa = 4.11LASLLINQGYY34 pKa = 6.94TPEE37 pKa = 4.25VVAGFYY43 pKa = 10.38PEE45 pKa = 5.21NGGQTVTFEE54 pKa = 3.31QSYY57 pKa = 9.35AVRR60 pKa = 11.84VEE62 pKa = 4.59TYY64 pKa = 10.77AEE66 pKa = 4.44CIKK69 pKa = 10.48LAQLAGDD76 pKa = 4.82AFQQEE81 pKa = 4.89CVLVVDD87 pKa = 4.33VLDD90 pKa = 4.7RR91 pKa = 11.84MAASYY96 pKa = 9.66LYY98 pKa = 9.75PAGDD102 pKa = 3.83STTEE106 pKa = 3.95GYY108 pKa = 10.36LRR110 pKa = 11.84RR111 pKa = 11.84QTEE114 pKa = 3.92QPEE117 pKa = 4.58GARR120 pKa = 11.84SQWRR124 pKa = 11.84GAWWTIEE131 pKa = 3.78ATAA134 pKa = 4.38

Molecular weight: 14.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9RYG7|K9RYG7_9CAUD Uncharacterized protein OS=Cronobacter phage vB_CskP_GAP227 OX=1264737 GN=GAP227_11 PE=4 SV=1
MM1 pKa = 7.64TKK3 pKa = 7.5TQRR6 pKa = 11.84RR7 pKa = 11.84INKK10 pKa = 9.32RR11 pKa = 11.84NLIRR15 pKa = 11.84NSRR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 9.5RR21 pKa = 11.84VVTGEE26 pKa = 4.21GNGHH30 pKa = 5.95YY31 pKa = 10.06FPVGTIVRR39 pKa = 11.84HH40 pKa = 6.16ADD42 pKa = 3.11AEE44 pKa = 4.43HH45 pKa = 6.14YY46 pKa = 11.0ADD48 pKa = 3.5VGGSEE53 pKa = 4.15TEE55 pKa = 4.13YY56 pKa = 10.99NFLVVEE62 pKa = 4.28TGKK65 pKa = 10.02VRR67 pKa = 11.84FPLKK71 pKa = 10.01QWVSPRR77 pKa = 11.84DD78 pKa = 3.42IAA80 pKa = 5.36

Molecular weight: 9.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

13031

38

1265

265.9

29.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.383 ± 0.568

1.082 ± 0.188

6.293 ± 0.207

6.362 ± 0.393

3.637 ± 0.134

7.843 ± 0.265

1.911 ± 0.179

4.558 ± 0.292

5.963 ± 0.29

8.18 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.077 ± 0.19

4.351 ± 0.247

3.906 ± 0.238

4.62 ± 0.39

5.679 ± 0.217

4.896 ± 0.262

5.571 ± 0.389

6.722 ± 0.26

1.427 ± 0.11

3.538 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski