Sewage-associated circular DNA virus-23

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UH82|A0A0B4UH82_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-23 OX=1592090 PE=3 SV=1
MM1 pKa = 7.71TSRR4 pKa = 11.84KK5 pKa = 7.75WVFTVNNYY13 pKa = 9.04TDD15 pKa = 5.17DD16 pKa = 3.29EE17 pKa = 4.48WNEE20 pKa = 3.78LKK22 pKa = 10.33RR23 pKa = 11.84WCMVNTKK30 pKa = 9.52YY31 pKa = 10.99AIMGKK36 pKa = 9.13EE37 pKa = 3.98VGPQCGTPHH46 pKa = 5.4IQGYY50 pKa = 10.2LSANKK55 pKa = 8.29PHH57 pKa = 6.8RR58 pKa = 11.84RR59 pKa = 11.84SGMVEE64 pKa = 3.5VCRR67 pKa = 11.84RR68 pKa = 11.84ARR70 pKa = 11.84WEE72 pKa = 3.74AAKK75 pKa = 11.14GNDD78 pKa = 3.7TQNDD82 pKa = 4.2DD83 pKa = 4.13YY84 pKa = 11.32CRR86 pKa = 11.84KK87 pKa = 9.16AAKK90 pKa = 9.72EE91 pKa = 3.75GRR93 pKa = 11.84EE94 pKa = 4.27VWRR97 pKa = 11.84HH98 pKa = 4.63GQPCTGAGQRR108 pKa = 11.84TDD110 pKa = 3.79LLRR113 pKa = 11.84VAAAVDD119 pKa = 3.55SGVSLRR125 pKa = 11.84EE126 pKa = 3.75IASEE130 pKa = 4.0NPSEE134 pKa = 4.27FIKK137 pKa = 9.92FHH139 pKa = 6.63RR140 pKa = 11.84GILAYY145 pKa = 10.41RR146 pKa = 11.84NLVRR150 pKa = 11.84PAVPRR155 pKa = 11.84DD156 pKa = 3.78FKK158 pKa = 11.11TEE160 pKa = 3.51LHH162 pKa = 6.19VFVGPPGSGKK172 pKa = 8.75SRR174 pKa = 11.84RR175 pKa = 11.84AQEE178 pKa = 4.03LASSFGTVYY187 pKa = 10.57RR188 pKa = 11.84KK189 pKa = 10.03VRR191 pKa = 11.84GPWWNGYY198 pKa = 7.37EE199 pKa = 4.06QQQSVIIDD207 pKa = 3.88DD208 pKa = 4.55FYY210 pKa = 11.41GWVPFDD216 pKa = 3.47EE217 pKa = 4.9LLRR220 pKa = 11.84VADD223 pKa = 5.18RR224 pKa = 11.84YY225 pKa = 7.84QHH227 pKa = 4.75QVEE230 pKa = 4.22IKK232 pKa = 10.67GGFEE236 pKa = 4.01EE237 pKa = 4.88FNSKK241 pKa = 10.32IIIITSNRR249 pKa = 11.84PIKK252 pKa = 10.28DD253 pKa = 2.71WYY255 pKa = 10.85KK256 pKa = 10.13FDD258 pKa = 4.69SYY260 pKa = 11.67DD261 pKa = 3.38PEE263 pKa = 4.8ALYY266 pKa = 10.49RR267 pKa = 11.84RR268 pKa = 11.84CSVYY272 pKa = 9.42QHH274 pKa = 6.51INMDD278 pKa = 3.65HH279 pKa = 6.51SVTDD283 pKa = 3.61YY284 pKa = 11.47EE285 pKa = 4.05IMEE288 pKa = 4.94GIKK291 pKa = 10.11INYY294 pKa = 8.27

Molecular weight: 34.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UH82|A0A0B4UH82_9VIRU ATP-dependent helicase Rep OS=Sewage-associated circular DNA virus-23 OX=1592090 PE=3 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84YY4 pKa = 8.27PKK6 pKa = 10.7ASGNRR11 pKa = 11.84YY12 pKa = 9.27APYY15 pKa = 9.93IRR17 pKa = 11.84TAGRR21 pKa = 11.84MYY23 pKa = 10.83GAAKK27 pKa = 9.6KK28 pKa = 10.53AYY30 pKa = 9.45GIYY33 pKa = 9.98SKK35 pKa = 10.99VRR37 pKa = 11.84TRR39 pKa = 11.84YY40 pKa = 9.07RR41 pKa = 11.84NSGSRR46 pKa = 11.84TKK48 pKa = 10.49QKK50 pKa = 10.4RR51 pKa = 11.84NYY53 pKa = 7.86RR54 pKa = 11.84KK55 pKa = 10.04KK56 pKa = 10.12YY57 pKa = 7.76GGAMGSSIPGRR68 pKa = 11.84CGIYY72 pKa = 9.64VRR74 pKa = 11.84KK75 pKa = 9.57GRR77 pKa = 11.84KK78 pKa = 8.99DD79 pKa = 2.92RR80 pKa = 11.84WLKK83 pKa = 10.87NLTKK87 pKa = 10.59NSPPRR92 pKa = 11.84LLLNQSTNQVEE103 pKa = 4.23SDD105 pKa = 3.15AGKK108 pKa = 10.38QGTLSILQTLTRR120 pKa = 11.84TDD122 pKa = 4.12FMNMKK127 pKa = 8.87ATLPAISDD135 pKa = 3.52TGLGSGAAVGFATVSYY151 pKa = 11.19YY152 pKa = 10.17MDD154 pKa = 3.79IALKK158 pKa = 10.19RR159 pKa = 11.84LKK161 pKa = 10.9AEE163 pKa = 3.7ILITNTCNSVVFVKK177 pKa = 10.07MRR179 pKa = 11.84TWLCKK184 pKa = 9.9KK185 pKa = 10.26SSNASPLEE193 pKa = 3.75KK194 pKa = 9.42WGEE197 pKa = 3.82ILNVQNANVIPANKK211 pKa = 9.79VLTTTYY217 pKa = 10.71GATPYY222 pKa = 10.08MVDD225 pKa = 5.39GFNTWWKK232 pKa = 10.31CVQTRR237 pKa = 11.84NLRR240 pKa = 11.84LAPGEE245 pKa = 4.3IYY247 pKa = 10.61CHH249 pKa = 5.05NHH251 pKa = 5.24IDD253 pKa = 4.23KK254 pKa = 10.41INKK257 pKa = 8.96IIRR260 pKa = 11.84GSSYY264 pKa = 9.93EE265 pKa = 4.0AEE267 pKa = 3.92QNLYY271 pKa = 10.03IRR273 pKa = 11.84GLSKK277 pKa = 10.78VVTITIHH284 pKa = 6.88GEE286 pKa = 3.72PTQDD290 pKa = 3.04STGVNTLSGAVVNVAGKK307 pKa = 10.27RR308 pKa = 11.84KK309 pKa = 7.74PTQIHH314 pKa = 5.77

Molecular weight: 34.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

608

294

314

304.0

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.908 ± 0.549

1.809 ± 0.162

4.112 ± 1.167

4.605 ± 1.535

2.467 ± 0.89

8.059 ± 0.402

1.974 ± 0.522

6.086 ± 0.212

7.237 ± 1.016

5.592 ± 1.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.183

5.921 ± 0.81

4.112 ± 0.216

3.618 ± 0.324

8.059 ± 0.548

6.414 ± 0.442

6.414 ± 1.629

6.743 ± 0.517

2.303 ± 0.53

5.263 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski