Skermanella stibiiresistens SB22

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Skermanella; Skermanella stibiiresistens

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9H9D2|W9H9D2_9PROT Molybdopterin molybdenumtransferase OS=Skermanella stibiiresistens SB22 OX=1385369 GN=N825_34615 PE=3 SV=1
MM1 pKa = 7.63AGAALAVAHH10 pKa = 5.66SHH12 pKa = 6.46GALAQMTVKK21 pKa = 10.77LGGYY25 pKa = 4.57TTFRR29 pKa = 11.84AATYY33 pKa = 10.8DD34 pKa = 3.2SDD36 pKa = 4.94APGATSRR43 pKa = 11.84EE44 pKa = 3.99FQNEE48 pKa = 4.05VEE50 pKa = 4.31IHH52 pKa = 5.47VSADD56 pKa = 2.95GKK58 pKa = 11.02ADD60 pKa = 3.39NGLLYY65 pKa = 10.34GAHH68 pKa = 6.89IEE70 pKa = 4.13FEE72 pKa = 4.27NDD74 pKa = 3.06GGDD77 pKa = 3.48GAGAGISVDD86 pKa = 3.51EE87 pKa = 4.43ASVYY91 pKa = 10.95VGGTWGRR98 pKa = 11.84IEE100 pKa = 5.8LGDD103 pKa = 3.89HH104 pKa = 7.37DD105 pKa = 4.71GASDD109 pKa = 3.53QLFVYY114 pKa = 10.49APTVGNGQIEE124 pKa = 4.38EE125 pKa = 4.16DD126 pKa = 3.71WYY128 pKa = 10.88DD129 pKa = 3.39FAGPGIEE136 pKa = 4.51TDD138 pKa = 4.78DD139 pKa = 4.69LLLPTDD145 pKa = 4.08TSDD148 pKa = 3.49STKK151 pKa = 8.53ITYY154 pKa = 7.51MSPVLGGFQAGVSYY168 pKa = 9.86TPQFDD173 pKa = 3.66SQGQDD178 pKa = 2.99VVRR181 pKa = 11.84FKK183 pKa = 10.12PTSPDD188 pKa = 3.11AEE190 pKa = 4.19NLYY193 pKa = 10.78KK194 pKa = 10.68DD195 pKa = 4.8LIEE198 pKa = 5.49AGAGYY203 pKa = 10.2KK204 pKa = 9.73RR205 pKa = 11.84DD206 pKa = 3.59IGPVSLVLGVGYY218 pKa = 9.98VHH220 pKa = 7.47GDD222 pKa = 3.18ANQGTDD228 pKa = 5.04AEE230 pKa = 5.65DD231 pKa = 3.44FDD233 pKa = 4.3AWGAGAQIGYY243 pKa = 10.18EE244 pKa = 4.11GFTIGGAYY252 pKa = 9.8NDD254 pKa = 4.56NGDD257 pKa = 4.04SLIEE261 pKa = 4.04TDD263 pKa = 4.74EE264 pKa = 4.67PDD266 pKa = 3.34AKK268 pKa = 10.37SWNVGVSYY276 pKa = 10.46EE277 pKa = 4.03ADD279 pKa = 3.34DD280 pKa = 3.72WGVAAQYY287 pKa = 10.94QKK289 pKa = 11.21VDD291 pKa = 3.55LPGRR295 pKa = 11.84KK296 pKa = 8.82INIYY300 pKa = 10.33GLGAAYY306 pKa = 9.61EE307 pKa = 4.14PAPGLTIGPEE317 pKa = 4.17VIFFDD322 pKa = 5.75DD323 pKa = 4.97DD324 pKa = 3.94DD325 pKa = 5.99PEE327 pKa = 5.27VGDD330 pKa = 4.72GYY332 pKa = 11.23VALIAVNLEE341 pKa = 4.03FF342 pKa = 6.0

Molecular weight: 35.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9GX22|W9GX22_9PROT Uncharacterized protein OS=Skermanella stibiiresistens SB22 OX=1385369 GN=N825_21450 PE=4 SV=1
MM1 pKa = 7.57ASSAAVRR8 pKa = 11.84FNILAAQIRR17 pKa = 11.84MMLTFFRR24 pKa = 11.84VSRR27 pKa = 11.84FFVLRR32 pKa = 3.85

Molecular weight: 3.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7269

0

7269

2267981

26

8112

312.0

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.259 ± 0.041

0.824 ± 0.011

5.94 ± 0.027

5.538 ± 0.029

3.512 ± 0.02

8.953 ± 0.032

2.09 ± 0.016

5.072 ± 0.021

2.813 ± 0.023

10.344 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.016

2.44 ± 0.018

5.368 ± 0.026

2.896 ± 0.018

7.462 ± 0.04

5.226 ± 0.027

5.597 ± 0.048

7.812 ± 0.023

1.3 ± 0.012

2.019 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski