Pantoea phage vB_PagM_LIET2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Lietduovirus; Pantoea virus LIET2

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 131 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AVZ7|A0A411AVZ7_9CAUD Ig-like domain-containing structural protein OS=Pantoea phage vB_PagM_LIET2 OX=2508071 GN=LIET2_gp030 PE=4 SV=1
MM1 pKa = 7.04NQLNEE6 pKa = 4.19EE7 pKa = 4.13QAHH10 pKa = 5.55LLKK13 pKa = 10.11TVMGSMWSDD22 pKa = 3.84FVAEE26 pKa = 4.05AEE28 pKa = 4.33EE29 pKa = 4.2NGFTEE34 pKa = 4.56EE35 pKa = 4.33DD36 pKa = 4.0CEE38 pKa = 4.07ALYY41 pKa = 10.81AAVGGEE47 pKa = 3.98NN48 pKa = 3.37

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AW01|A0A411AW01_9CAUD Uncharacterized protein OS=Pantoea phage vB_PagM_LIET2 OX=2508071 GN=LIET2_gp014 PE=4 SV=1
MM1 pKa = 7.63AGLSRR6 pKa = 11.84LLTMGFFAKK15 pKa = 10.53GFFWTLGKK23 pKa = 10.16LAAFAFTHH31 pKa = 5.64FVVFILALIILGFMAFRR48 pKa = 11.84GRR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 9.77KK53 pKa = 10.66

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

131

0

131

24067

28

1218

183.7

20.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.118 ± 0.323

1.209 ± 0.12

6.004 ± 0.145

5.933 ± 0.246

3.74 ± 0.132

7.649 ± 0.241

1.99 ± 0.125

5.95 ± 0.148

5.318 ± 0.154

7.313 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.133 ± 0.123

4.355 ± 0.157

4.575 ± 0.213

3.881 ± 0.171

5.834 ± 0.274

5.722 ± 0.166

6.233 ± 0.261

6.652 ± 0.182

1.625 ± 0.105

2.767 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski