Amino acid dipepetide frequency for Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.714AlaAla: 5.714 ± 0.098
0.924AlaCys: 0.924 ± 0.028
3.676AlaAsp: 3.676 ± 0.056
5.424AlaGlu: 5.424 ± 0.08
3.11AlaPhe: 3.11 ± 0.064
6.032AlaGly: 6.032 ± 0.089
1.044AlaHis: 1.044 ± 0.03
4.714AlaIle: 4.714 ± 0.065
3.779AlaLys: 3.779 ± 0.06
7.16AlaLeu: 7.16 ± 0.118
1.662AlaMet: 1.662 ± 0.036
2.461AlaAsn: 2.461 ± 0.052
2.247AlaPro: 2.247 ± 0.047
1.587AlaGln: 1.587 ± 0.036
3.235AlaArg: 3.235 ± 0.053
4.559AlaSer: 4.559 ± 0.07
3.221AlaThr: 3.221 ± 0.056
5.575AlaVal: 5.575 ± 0.073
0.735AlaTrp: 0.735 ± 0.031
2.4AlaTyr: 2.4 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.747CysAla: 0.747 ± 0.025
0.231CysCys: 0.231 ± 0.017
0.619CysAsp: 0.619 ± 0.024
0.738CysGlu: 0.738 ± 0.027
0.563CysPhe: 0.563 ± 0.02
1.225CysGly: 1.225 ± 0.033
0.243CysHis: 0.243 ± 0.015
0.836CysIle: 0.836 ± 0.027
0.728CysLys: 0.728 ± 0.026
1.006CysLeu: 1.006 ± 0.027
0.349CysMet: 0.349 ± 0.016
0.49CysAsn: 0.49 ± 0.018
0.764CysPro: 0.764 ± 0.029
0.297CysGln: 0.297 ± 0.017
0.718CysArg: 0.718 ± 0.027
0.897CysSer: 0.897 ± 0.032
0.747CysThr: 0.747 ± 0.03
0.727CysVal: 0.727 ± 0.027
0.113CysTrp: 0.113 ± 0.008
0.467CysTyr: 0.467 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
3.463AspAla: 3.463 ± 0.062
0.655AspCys: 0.655 ± 0.025
2.227AspAsp: 2.227 ± 0.058
3.97AspGlu: 3.97 ± 0.06
2.876AspPhe: 2.876 ± 0.045
3.93AspGly: 3.93 ± 0.099
0.794AspHis: 0.794 ± 0.021
4.386AspIle: 4.386 ± 0.06
2.836AspLys: 2.836 ± 0.05
5.209AspLeu: 5.209 ± 0.078
1.365AspMet: 1.365 ± 0.032
2.138AspAsn: 2.138 ± 0.048
2.579AspPro: 2.579 ± 0.046
1.194AspGln: 1.194 ± 0.031
2.603AspArg: 2.603 ± 0.047
3.998AspSer: 3.998 ± 0.073
2.794AspThr: 2.794 ± 0.056
3.653AspVal: 3.653 ± 0.058
0.613AspTrp: 0.613 ± 0.023
2.215AspTyr: 2.215 ± 0.045
0.001AspXaa: 0.001 ± 0.001
Glu
5.674GluAla: 5.674 ± 0.09
0.737GluCys: 0.737 ± 0.026
4.118GluAsp: 4.118 ± 0.049
7.33GluGlu: 7.33 ± 0.103
2.975GluPhe: 2.975 ± 0.056
4.824GluGly: 4.824 ± 0.061
1.148GluHis: 1.148 ± 0.03
6.491GluIle: 6.491 ± 0.09
7.187GluLys: 7.187 ± 0.117
7.488GluLeu: 7.488 ± 0.106
2.116GluMet: 2.116 ± 0.046
4.788GluAsn: 4.788 ± 0.088
2.39GluPro: 2.39 ± 0.045
2.004GluGln: 2.004 ± 0.045
3.255GluArg: 3.255 ± 0.067
4.375GluSer: 4.375 ± 0.069
4.306GluThr: 4.306 ± 0.06
4.9GluVal: 4.9 ± 0.059
0.774GluTrp: 0.774 ± 0.025
2.775GluTyr: 2.775 ± 0.049
0.001GluXaa: 0.001 ± 0.001
Phe
2.807PheAla: 2.807 ± 0.055
0.67PheCys: 0.67 ± 0.024
2.544PheAsp: 2.544 ± 0.052
3.343PheGlu: 3.343 ± 0.068
2.224PhePhe: 2.224 ± 0.057
3.27PheGly: 3.27 ± 0.057
0.751PheHis: 0.751 ± 0.022
2.949PheIle: 2.949 ± 0.056
2.188PheLys: 2.188 ± 0.044
4.51PheLeu: 4.51 ± 0.071
1.031PheMet: 1.031 ± 0.029
1.674PheAsn: 1.674 ± 0.041
1.778PhePro: 1.778 ± 0.039
1.068PheGln: 1.068 ± 0.027
2.166PheArg: 2.166 ± 0.049
3.762PheSer: 3.762 ± 0.063
2.601PheThr: 2.601 ± 0.062
2.908PheVal: 2.908 ± 0.049
0.475PheTrp: 0.475 ± 0.017
1.617PheTyr: 1.617 ± 0.038
0.001PheXaa: 0.001 ± 0.001
Gly
4.718GlyAla: 4.718 ± 0.081
1.136GlyCys: 1.136 ± 0.03
3.775GlyAsp: 3.775 ± 0.071
5.061GlyGlu: 5.061 ± 0.068
3.424GlyPhe: 3.424 ± 0.058
4.842GlyGly: 4.842 ± 0.096
1.175GlyHis: 1.175 ± 0.03
6.244GlyIle: 6.244 ± 0.074
5.595GlyLys: 5.595 ± 0.074
6.127GlyLeu: 6.127 ± 0.069
2.027GlyMet: 2.027 ± 0.043
3.659GlyAsn: 3.659 ± 0.079
2.021GlyPro: 2.021 ± 0.045
1.58GlyGln: 1.58 ± 0.034
2.913GlyArg: 2.913 ± 0.05
5.096GlySer: 5.096 ± 0.092
4.61GlyThr: 4.61 ± 0.093
4.799GlyVal: 4.799 ± 0.065
0.819GlyTrp: 0.819 ± 0.027
3.009GlyTyr: 3.009 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
1.059HisAla: 1.059 ± 0.026
0.267HisCys: 0.267 ± 0.015
0.87HisAsp: 0.87 ± 0.028
1.136HisGlu: 1.136 ± 0.032
0.802HisPhe: 0.802 ± 0.025
1.171HisGly: 1.171 ± 0.03
0.381HisHis: 0.381 ± 0.022
1.204HisIle: 1.204 ± 0.031
0.843HisLys: 0.843 ± 0.024
1.472HisLeu: 1.472 ± 0.038
0.427HisMet: 0.427 ± 0.018
0.674HisAsn: 0.674 ± 0.023
0.965HisPro: 0.965 ± 0.024
0.428HisGln: 0.428 ± 0.019
0.907HisArg: 0.907 ± 0.027
1.1HisSer: 1.1 ± 0.035
0.946HisThr: 0.946 ± 0.03
1.105HisVal: 1.105 ± 0.031
0.201HisTrp: 0.201 ± 0.013
0.628HisTyr: 0.628 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.122IleAla: 5.122 ± 0.075
0.994IleCys: 0.994 ± 0.029
3.945IleAsp: 3.945 ± 0.061
5.573IleGlu: 5.573 ± 0.075
3.591IlePhe: 3.591 ± 0.062
5.259IleGly: 5.259 ± 0.074
1.27IleHis: 1.27 ± 0.035
5.022IleIle: 5.022 ± 0.077
4.452IleLys: 4.452 ± 0.072
6.827IleLeu: 6.827 ± 0.093
1.624IleMet: 1.624 ± 0.033
3.042IleAsn: 3.042 ± 0.05
3.509IlePro: 3.509 ± 0.054
1.898IleGln: 1.898 ± 0.045
3.445IleArg: 3.445 ± 0.054
5.627IleSer: 5.627 ± 0.064
4.069IleThr: 4.069 ± 0.06
4.758IleVal: 4.758 ± 0.068
0.69IleTrp: 0.69 ± 0.023
2.783IleTyr: 2.783 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
4.651LysAla: 4.651 ± 0.065
0.679LysCys: 0.679 ± 0.026
3.359LysAsp: 3.359 ± 0.055
5.878LysGlu: 5.878 ± 0.09
2.11LysPhe: 2.11 ± 0.043
4.151LysGly: 4.151 ± 0.061
1.049LysHis: 1.049 ± 0.03
5.083LysIle: 5.083 ± 0.076
5.591LysLys: 5.591 ± 0.106
5.617LysLeu: 5.617 ± 0.082
1.732LysMet: 1.732 ± 0.04
3.6LysAsn: 3.6 ± 0.058
2.288LysPro: 2.288 ± 0.043
1.715LysGln: 1.715 ± 0.048
2.846LysArg: 2.846 ± 0.057
3.7LysSer: 3.7 ± 0.053
3.725LysThr: 3.725 ± 0.054
4.25LysVal: 4.25 ± 0.06
0.638LysTrp: 0.638 ± 0.024
2.312LysTyr: 2.312 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
6.733LeuAla: 6.733 ± 0.078
1.026LeuCys: 1.026 ± 0.031
5.105LeuAsp: 5.105 ± 0.069
7.792LeuGlu: 7.792 ± 0.121
4.233LeuPhe: 4.233 ± 0.072
6.504LeuGly: 6.504 ± 0.085
1.543LeuHis: 1.543 ± 0.04
6.695LeuIle: 6.695 ± 0.083
6.847LeuLys: 6.847 ± 0.093
9.17LeuLeu: 9.17 ± 0.122
2.279LeuMet: 2.279 ± 0.048
4.019LeuAsn: 4.019 ± 0.063
3.866LeuPro: 3.866 ± 0.052
2.539LeuGln: 2.539 ± 0.046
4.018LeuArg: 4.018 ± 0.057
6.843LeuSer: 6.843 ± 0.079
4.956LeuThr: 4.956 ± 0.077
5.866LeuVal: 5.866 ± 0.079
0.881LeuTrp: 0.881 ± 0.026
3.142LeuTyr: 3.142 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
1.646MetAla: 1.646 ± 0.044
0.247MetCys: 0.247 ± 0.013
1.408MetAsp: 1.408 ± 0.031
1.908MetGlu: 1.908 ± 0.044
0.919MetPhe: 0.919 ± 0.026
1.89MetGly: 1.89 ± 0.044
0.447MetHis: 0.447 ± 0.02
1.668MetIle: 1.668 ± 0.038
2.02MetLys: 2.02 ± 0.043
2.35MetLeu: 2.35 ± 0.047
0.652MetMet: 0.652 ± 0.023
1.127MetAsn: 1.127 ± 0.024
0.986MetPro: 0.986 ± 0.026
0.799MetGln: 0.799 ± 0.025
1.151MetArg: 1.151 ± 0.028
1.475MetSer: 1.475 ± 0.034
1.301MetThr: 1.301 ± 0.041
1.603MetVal: 1.603 ± 0.031
0.201MetTrp: 0.201 ± 0.014
0.786MetTyr: 0.786 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.856AsnAla: 2.856 ± 0.056
0.616AsnCys: 0.616 ± 0.03
1.815AsnAsp: 1.815 ± 0.043
3.028AsnGlu: 3.028 ± 0.052
2.0AsnPhe: 2.0 ± 0.049
3.117AsnGly: 3.117 ± 0.075
0.63AsnHis: 0.63 ± 0.022
3.449AsnIle: 3.449 ± 0.059
2.33AsnLys: 2.33 ± 0.045
4.454AsnLeu: 4.454 ± 0.07
1.132AsnMet: 1.132 ± 0.033
2.25AsnAsn: 2.25 ± 0.081
2.572AsnPro: 2.572 ± 0.049
1.1AsnGln: 1.1 ± 0.028
2.119AsnArg: 2.119 ± 0.045
3.402AsnSer: 3.402 ± 0.062
2.507AsnThr: 2.507 ± 0.06
3.086AsnVal: 3.086 ± 0.064
0.48AsnTrp: 0.48 ± 0.022
1.798AsnTyr: 1.798 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.022ProAla: 3.022 ± 0.053
0.465ProCys: 0.465 ± 0.021
2.84ProAsp: 2.84 ± 0.054
4.917ProGlu: 4.917 ± 0.082
1.698ProPhe: 1.698 ± 0.037
3.117ProGly: 3.117 ± 0.056
0.651ProHis: 0.651 ± 0.024
2.255ProIle: 2.255 ± 0.043
1.897ProLys: 1.897 ± 0.041
3.661ProLeu: 3.661 ± 0.059
0.853ProMet: 0.853 ± 0.024
1.201ProAsn: 1.201 ± 0.031
1.294ProPro: 1.294 ± 0.033
1.029ProGln: 1.029 ± 0.028
1.408ProArg: 1.408 ± 0.041
2.433ProSer: 2.433 ± 0.05
1.857ProThr: 1.857 ± 0.04
3.577ProVal: 3.577 ± 0.07
0.384ProTrp: 0.384 ± 0.017
1.505ProTyr: 1.505 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
1.963GlnAla: 1.963 ± 0.045
0.28GlnCys: 0.28 ± 0.016
1.189GlnAsp: 1.189 ± 0.031
2.127GlnGlu: 2.127 ± 0.047
0.997GlnPhe: 0.997 ± 0.033
1.613GlnGly: 1.613 ± 0.037
0.425GlnHis: 0.425 ± 0.02
2.0GlnIle: 2.0 ± 0.043
2.112GlnLys: 2.112 ± 0.039
2.322GlnLeu: 2.322 ± 0.049
0.667GlnMet: 0.667 ± 0.023
1.39GlnAsn: 1.39 ± 0.04
0.86GlnPro: 0.86 ± 0.026
0.735GlnGln: 0.735 ± 0.026
1.1GlnArg: 1.1 ± 0.03
1.393GlnSer: 1.393 ± 0.036
1.295GlnThr: 1.295 ± 0.034
1.783GlnVal: 1.783 ± 0.041
0.212GlnTrp: 0.212 ± 0.012
0.863GlnTyr: 0.863 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
2.979ArgAla: 2.979 ± 0.055
0.59ArgCys: 0.59 ± 0.026
2.475ArgAsp: 2.475 ± 0.043
3.825ArgGlu: 3.825 ± 0.071
1.987ArgPhe: 1.987 ± 0.037
2.678ArgGly: 2.678 ± 0.048
0.772ArgHis: 0.772 ± 0.025
3.704ArgIle: 3.704 ± 0.06
3.765ArgLys: 3.765 ± 0.07
3.916ArgLeu: 3.916 ± 0.069
1.194ArgMet: 1.194 ± 0.026
2.084ArgAsn: 2.084 ± 0.039
1.426ArgPro: 1.426 ± 0.037
1.281ArgGln: 1.281 ± 0.032
2.069ArgArg: 2.069 ± 0.04
2.458ArgSer: 2.458 ± 0.041
2.176ArgThr: 2.176 ± 0.044
3.009ArgVal: 3.009 ± 0.051
0.468ArgTrp: 0.468 ± 0.019
1.795ArgTyr: 1.795 ± 0.041
0.001ArgXaa: 0.001 ± 0.001
Ser
4.372SerAla: 4.372 ± 0.079
0.764SerCys: 0.764 ± 0.022
3.737SerAsp: 3.737 ± 0.067
5.299SerGlu: 5.299 ± 0.073
3.139SerPhe: 3.139 ± 0.056
6.152SerGly: 6.152 ± 0.081
1.164SerHis: 1.164 ± 0.032
4.885SerIle: 4.885 ± 0.066
3.787SerLys: 3.787 ± 0.063
6.235SerLeu: 6.235 ± 0.071
1.65SerMet: 1.65 ± 0.036
2.76SerAsn: 2.76 ± 0.066
3.011SerPro: 3.011 ± 0.053
1.707SerGln: 1.707 ± 0.033
3.303SerArg: 3.303 ± 0.057
5.503SerSer: 5.503 ± 0.102
3.86SerThr: 3.86 ± 0.097
4.503SerVal: 4.503 ± 0.066
0.891SerTrp: 0.891 ± 0.034
2.351SerTyr: 2.351 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
4.064ThrAla: 4.064 ± 0.075
0.652ThrCys: 0.652 ± 0.024
3.149ThrAsp: 3.149 ± 0.073
3.922ThrGlu: 3.922 ± 0.061
2.216ThrPhe: 2.216 ± 0.042
5.086ThrGly: 5.086 ± 0.094
0.894ThrHis: 0.894 ± 0.023
3.853ThrIle: 3.853 ± 0.066
2.564ThrLys: 2.564 ± 0.042
5.237ThrLeu: 5.237 ± 0.063
1.057ThrMet: 1.057 ± 0.025
2.106ThrAsn: 2.106 ± 0.056
2.519ThrPro: 2.519 ± 0.043
1.258ThrGln: 1.258 ± 0.03
2.093ThrArg: 2.093 ± 0.04
3.619ThrSer: 3.619 ± 0.095
2.745ThrThr: 2.745 ± 0.065
4.625ThrVal: 4.625 ± 0.113
0.547ThrTrp: 0.547 ± 0.022
2.211ThrTyr: 2.211 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
4.56ValAla: 4.56 ± 0.065
0.949ValCys: 0.949 ± 0.028
3.73ValAsp: 3.73 ± 0.06
5.023ValGlu: 5.023 ± 0.072
3.179ValPhe: 3.179 ± 0.05
4.254ValGly: 4.254 ± 0.067
1.304ValHis: 1.304 ± 0.031
4.834ValIle: 4.834 ± 0.061
4.056ValLys: 4.056 ± 0.058
6.857ValLeu: 6.857 ± 0.082
1.625ValMet: 1.625 ± 0.033
2.914ValAsn: 2.914 ± 0.049
3.024ValPro: 3.024 ± 0.049
1.796ValGln: 1.796 ± 0.036
3.034ValArg: 3.034 ± 0.057
5.347ValSer: 5.347 ± 0.082
3.98ValThr: 3.98 ± 0.1
4.67ValVal: 4.67 ± 0.075
0.73ValTrp: 0.73 ± 0.037
2.56ValTyr: 2.56 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.696TrpAla: 0.696 ± 0.028
0.126TrpCys: 0.126 ± 0.009
0.772TrpAsp: 0.772 ± 0.035
0.797TrpGlu: 0.797 ± 0.027
0.403TrpPhe: 0.403 ± 0.017
0.709TrpGly: 0.709 ± 0.027
0.209TrpHis: 0.209 ± 0.013
0.764TrpIle: 0.764 ± 0.024
0.756TrpLys: 0.756 ± 0.027
0.885TrpLeu: 0.885 ± 0.028
0.268TrpMet: 0.268 ± 0.014
0.67TrpAsn: 0.67 ± 0.029
0.259TrpPro: 0.259 ± 0.014
0.335TrpGln: 0.335 ± 0.019
0.393TrpArg: 0.393 ± 0.017
0.629TrpSer: 0.629 ± 0.026
0.608TrpThr: 0.608 ± 0.022
0.657TrpVal: 0.657 ± 0.024
0.152TrpTrp: 0.152 ± 0.011
0.427TrpTyr: 0.427 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.41TyrAla: 2.41 ± 0.056
0.498TyrCys: 0.498 ± 0.022
2.145TyrAsp: 2.145 ± 0.056
2.564TyrGlu: 2.564 ± 0.05
1.892TyrPhe: 1.892 ± 0.044
2.803TyrGly: 2.803 ± 0.056
0.69TyrHis: 0.69 ± 0.023
2.465TyrIle: 2.465 ± 0.048
1.643TyrLys: 1.643 ± 0.042
3.553TyrLeu: 3.553 ± 0.064
0.878TyrMet: 0.878 ± 0.03
1.694TyrAsn: 1.694 ± 0.042
1.702TyrPro: 1.702 ± 0.036
0.976TyrGln: 0.976 ± 0.027
1.874TyrArg: 1.874 ± 0.037
2.858TyrSer: 2.858 ± 0.056
2.114TyrThr: 2.114 ± 0.064
2.369TyrVal: 2.369 ± 0.047
0.514TyrTrp: 0.514 ± 0.021
1.539TyrTyr: 1.539 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.001
0.001XaaGlu: 0.001 ± 0.001
0.001XaaPhe: 0.001 ± 0.001
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.001XaaIle: 0.001 ± 0.001
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4468 proteins (1392316 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski