Paenibacillus sp. FSL R7-0331

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A089KWA7|A0A089KWA7_9BACL Membrane protein OS=Paenibacillus sp. FSL R7-0331 OX=1536773 GN=R70331_06810 PE=4 SV=1
MM1 pKa = 7.72AKK3 pKa = 8.78YY4 pKa = 8.25TWVEE8 pKa = 3.38KK9 pKa = 9.49DD10 pKa = 3.01TCIACGACGATAPDD24 pKa = 3.18IFDD27 pKa = 4.18YY28 pKa = 11.11DD29 pKa = 4.02DD30 pKa = 3.85EE31 pKa = 5.71GLAEE35 pKa = 4.97VIFEE39 pKa = 4.22SDD41 pKa = 3.39GNRR44 pKa = 11.84GCTVIPDD51 pKa = 4.21DD52 pKa = 5.96LFDD55 pKa = 5.5DD56 pKa = 4.87LQDD59 pKa = 4.12SADD62 pKa = 3.77GCPTDD67 pKa = 4.28SIKK70 pKa = 10.79IADD73 pKa = 3.66APFNKK78 pKa = 10.1EE79 pKa = 3.5GG80 pKa = 3.5

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A089L452|A0A089L452_9BACL Ribonuclease H OS=Paenibacillus sp. FSL R7-0331 OX=1536773 GN=R70331_21845 PE=3 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5436

0

5436

1788232

26

5496

329.0

36.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.812 ± 0.038

0.757 ± 0.01

4.996 ± 0.025

6.78 ± 0.042

4.056 ± 0.025

7.813 ± 0.031

1.956 ± 0.017

6.485 ± 0.033

4.892 ± 0.029

10.527 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.016

3.729 ± 0.026

4.023 ± 0.022

3.804 ± 0.02

5.015 ± 0.033

6.435 ± 0.028

5.411 ± 0.033

6.947 ± 0.029

1.209 ± 0.013

3.622 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski