Sphingomonas turrisvirgatae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3850 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3LYE5|A0A1E3LYE5_9SPHN Fatty acid desaturase OS=Sphingomonas turrisvirgatae OX=1888892 GN=BFL28_00575 PE=4 SV=1
MM1 pKa = 7.58RR2 pKa = 11.84ARR4 pKa = 11.84WHH6 pKa = 6.26LLGIVVAGSLAVGSCAGALAGNFTVTGGLYY36 pKa = 9.98PKK38 pKa = 10.15AGWGVPSFGSEE49 pKa = 3.86AEE51 pKa = 4.24IAHH54 pKa = 6.62LPAAAAVEE62 pKa = 4.35RR63 pKa = 11.84IEE65 pKa = 4.61PKK67 pKa = 10.1PAFAGYY73 pKa = 9.42YY74 pKa = 8.59YY75 pKa = 10.15PPEE78 pKa = 4.26VPVASSPQDD87 pKa = 3.13AALAASQIDD96 pKa = 4.15AIYY99 pKa = 10.4DD100 pKa = 3.56EE101 pKa = 4.74PQRR104 pKa = 11.84ATLDD108 pKa = 3.91DD109 pKa = 4.59PDD111 pKa = 3.78ARR113 pKa = 11.84EE114 pKa = 4.64AEE116 pKa = 4.32PQQAMVTSAMAPDD129 pKa = 3.71TGDD132 pKa = 3.57AGDD135 pKa = 4.14WPAPPAPSADD145 pKa = 3.53VQSYY149 pKa = 10.89SSYY152 pKa = 11.12SDD154 pKa = 3.13VAAAEE159 pKa = 4.29DD160 pKa = 3.51

Molecular weight: 16.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3M038|A0A1E3M038_9SPHN Uncharacterized protein OS=Sphingomonas turrisvirgatae OX=1888892 GN=BFL28_01360 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.45LSAA44 pKa = 3.94

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3850

0

3850

1203759

41

2551

312.7

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.676 ± 0.059

0.76 ± 0.012

5.933 ± 0.031

5.273 ± 0.035

3.46 ± 0.025

8.935 ± 0.041

1.919 ± 0.024

4.976 ± 0.027

2.906 ± 0.033

9.825 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.377 ± 0.021

2.493 ± 0.024

5.342 ± 0.03

3.177 ± 0.023

7.579 ± 0.046

5.113 ± 0.027

5.33 ± 0.031

7.302 ± 0.037

1.451 ± 0.019

2.171 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski