Ralstonia phage DU_RP_I

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Gyeongsanvirus; Ralstonia virus DURPI

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2W598|A0A2D2W598_9CAUD Uncharacterized protein OS=Ralstonia phage DU_RP_I OX=2041493 GN=R1B41kb_p032 PE=4 SV=1
MM1 pKa = 7.31SVDD4 pKa = 3.6SVVIKK9 pKa = 10.88QPDD12 pKa = 3.55APVEE16 pKa = 4.16DD17 pKa = 4.1QAHH20 pKa = 6.32IDD22 pKa = 3.51AMLAAVDD29 pKa = 3.77AANTSTEE36 pKa = 4.03PDD38 pKa = 3.63TPEE41 pKa = 3.8VPAEE45 pKa = 4.63GRR47 pKa = 11.84PQWLPEE53 pKa = 4.05KK54 pKa = 10.23FKK56 pKa = 11.38SPEE59 pKa = 4.03DD60 pKa = 3.39LAKK63 pKa = 10.54AYY65 pKa = 10.6AEE67 pKa = 4.39LEE69 pKa = 4.21GKK71 pKa = 10.38LGGKK75 pKa = 9.52KK76 pKa = 10.06DD77 pKa = 4.01DD78 pKa = 4.09ATPPADD84 pKa = 4.23DD85 pKa = 4.38KK86 pKa = 11.5AAKK89 pKa = 9.97SDD91 pKa = 3.91EE92 pKa = 4.33NPDD95 pKa = 3.65PSKK98 pKa = 10.61ATQDD102 pKa = 3.85DD103 pKa = 3.6ASKK106 pKa = 10.97ALSEE110 pKa = 4.52KK111 pKa = 10.53GLSFDD116 pKa = 3.55EE117 pKa = 5.33FSAEE121 pKa = 3.93FAQKK125 pKa = 11.16GEE127 pKa = 4.19LTAEE131 pKa = 4.26SYY133 pKa = 10.49EE134 pKa = 4.15KK135 pKa = 10.85LEE137 pKa = 4.08KK138 pKa = 10.72AGIPKK143 pKa = 10.29AVVDD147 pKa = 4.06QYY149 pKa = 11.66IAGQQALAEE158 pKa = 4.46SYY160 pKa = 10.46RR161 pKa = 11.84KK162 pKa = 10.1DD163 pKa = 3.48VTSVAGGDD171 pKa = 3.34EE172 pKa = 4.58SFAEE176 pKa = 4.29MVTWAAANLSKK187 pKa = 11.35EE188 pKa = 4.17EE189 pKa = 3.45IAAYY193 pKa = 10.25NKK195 pKa = 10.54AVDD198 pKa = 4.19SGDD201 pKa = 3.25INQAKK206 pKa = 9.79LVVAGVYY213 pKa = 9.36QKK215 pKa = 10.81FDD217 pKa = 3.06AAGRR221 pKa = 11.84GGEE224 pKa = 4.03PALVTGAGGKK234 pKa = 9.96VSGDD238 pKa = 3.52VYY240 pKa = 11.19EE241 pKa = 5.81SLAQMQKK248 pKa = 11.22DD249 pKa = 3.85MASLEE254 pKa = 4.4YY255 pKa = 9.53KK256 pKa = 9.64TDD258 pKa = 3.33PAFRR262 pKa = 11.84KK263 pKa = 9.92KK264 pKa = 10.63VEE266 pKa = 3.94QKK268 pKa = 9.78IARR271 pKa = 11.84SNILL275 pKa = 3.23

Molecular weight: 29.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2W504|A0A2D2W504_9CAUD Uncharacterized protein OS=Ralstonia phage DU_RP_I OX=2041493 GN=R1B41kb_p016 PE=4 SV=1
MM1 pKa = 6.72SQSRR5 pKa = 11.84KK6 pKa = 9.97GSLIEE11 pKa = 4.3ALINTAIGFGINFTANLIILPLFGFTSLTVQTNLVIGVVYY51 pKa = 8.91TLISVVRR58 pKa = 11.84SYY60 pKa = 10.87VVRR63 pKa = 11.84RR64 pKa = 11.84WFNAHH69 pKa = 5.98IVRR72 pKa = 11.84AAKK75 pKa = 10.18KK76 pKa = 10.44LSGAA80 pKa = 3.94

Molecular weight: 8.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

11211

53

1573

287.5

31.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.723 ± 0.436

0.767 ± 0.175

6.048 ± 0.24

6.235 ± 0.547

3.8 ± 0.219

8.179 ± 0.466

1.713 ± 0.199

4.772 ± 0.23

6.253 ± 0.407

8.349 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.222

4.299 ± 0.383

4.576 ± 0.196

3.943 ± 0.309

5.682 ± 0.324

5.664 ± 0.343

6.119 ± 0.487

7.02 ± 0.374

1.579 ± 0.152

3.006 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski