Holcus lanatus-associated virus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses

Average proteome isoelectric point is 8.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345BKB0|A0A345BKB0_9VIRU Coat protein OS=Holcus lanatus-associated virus OX=2282643 PE=4 SV=1
MM1 pKa = 7.73PRR3 pKa = 11.84QKK5 pKa = 10.77SPAHH9 pKa = 6.29DD10 pKa = 3.07WCFTINNPRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84DD22 pKa = 3.52HH23 pKa = 7.13QRR25 pKa = 11.84VQDD28 pKa = 3.3WQYY31 pKa = 11.76NYY33 pKa = 10.7LIYY36 pKa = 10.37QLEE39 pKa = 4.43VGDD42 pKa = 3.83QGTPHH47 pKa = 6.52LQGFVQFTTKK57 pKa = 9.92QRR59 pKa = 11.84LTALKK64 pKa = 9.67KK65 pKa = 9.51LHH67 pKa = 6.89KK68 pKa = 9.34SAHH71 pKa = 5.16WEE73 pKa = 3.76PRR75 pKa = 11.84RR76 pKa = 11.84GSAYY80 pKa = 9.8QASHH84 pKa = 5.95YY85 pKa = 10.01CEE87 pKa = 5.25KK88 pKa = 10.49PVPDD92 pKa = 5.09CDD94 pKa = 3.59CHH96 pKa = 7.87HH97 pKa = 6.89CVDD100 pKa = 4.07TPRR103 pKa = 11.84VYY105 pKa = 10.67PQFIFKK111 pKa = 10.18NGNISAPAGEE121 pKa = 4.77KK122 pKa = 8.95LTSIARR128 pKa = 11.84VIKK131 pKa = 9.99TKK133 pKa = 10.56GLSAAIDD140 pKa = 3.95NYY142 pKa = 7.55PTHH145 pKa = 6.72YY146 pKa = 10.01MGMNRR151 pKa = 11.84GMVNRR156 pKa = 11.84PPINYY161 pKa = 9.3KK162 pKa = 9.83EE163 pKa = 3.99ALANFYY169 pKa = 10.86SPQRR173 pKa = 11.84DD174 pKa = 3.24WQTVVSICYY183 pKa = 8.81GKK185 pKa = 9.9PEE187 pKa = 4.19TGKK190 pKa = 8.24TRR192 pKa = 11.84YY193 pKa = 10.36AMLAPSPYY201 pKa = 10.26KK202 pKa = 10.41LAAFGAEE209 pKa = 3.74GSTDD213 pKa = 3.55FFGDD217 pKa = 3.56YY218 pKa = 10.57RR219 pKa = 11.84PDD221 pKa = 3.18QHH223 pKa = 6.04EE224 pKa = 4.36TLVVDD229 pKa = 5.11DD230 pKa = 6.51FYY232 pKa = 11.94SNWKK236 pKa = 8.04YY237 pKa = 6.84TTFLQVCDD245 pKa = 4.41RR246 pKa = 11.84YY247 pKa = 8.69PTEE250 pKa = 3.81VHH252 pKa = 5.73TKK254 pKa = 9.97GGFRR258 pKa = 11.84QLLVRR263 pKa = 11.84HH264 pKa = 6.0IVFTSNHH271 pKa = 5.91SPGVWYY277 pKa = 9.29PNVLADD283 pKa = 3.61ADD285 pKa = 3.91RR286 pKa = 11.84RR287 pKa = 11.84EE288 pKa = 4.18SFNRR292 pKa = 11.84RR293 pKa = 11.84IHH295 pKa = 5.9NIIWFTPDD303 pKa = 3.27GYY305 pKa = 9.39WVKK308 pKa = 10.65KK309 pKa = 8.81GTLPWPCPFLQPLAWNHH326 pKa = 4.4VLRR329 pKa = 11.84NEE331 pKa = 4.11MLPIALLPQNQNLTIHH347 pKa = 6.18EE348 pKa = 4.46RR349 pKa = 11.84GILDD353 pKa = 4.06PSQLFVV359 pKa = 3.4

Molecular weight: 41.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345BKB0|A0A345BKB0_9VIRU Coat protein OS=Holcus lanatus-associated virus OX=2282643 PE=4 SV=1
MM1 pKa = 7.68GPMRR5 pKa = 11.84QYY7 pKa = 11.32KK8 pKa = 8.33PTGSPRR14 pKa = 11.84AGQRR18 pKa = 11.84AYY20 pKa = 9.92KK21 pKa = 9.82SVYY24 pKa = 8.6SRR26 pKa = 11.84RR27 pKa = 11.84PNRR30 pKa = 11.84PMMGPSNKK38 pKa = 9.51AVSLLKK44 pKa = 9.89MGYY47 pKa = 9.88LNRR50 pKa = 11.84RR51 pKa = 11.84GQLNRR56 pKa = 11.84EE57 pKa = 3.76TGYY60 pKa = 11.32VDD62 pKa = 4.96LASQVFNGNTTGTIVLAATIVQGAAVTQRR91 pKa = 11.84VGKK94 pKa = 9.76KK95 pKa = 8.99VLWKK99 pKa = 10.18GMQIRR104 pKa = 11.84GYY106 pKa = 10.19AQADD110 pKa = 3.44NTTLVTGGTWILVYY124 pKa = 10.4DD125 pKa = 4.21KK126 pKa = 10.6RR127 pKa = 11.84PTGALPAITDD137 pKa = 3.37ILVSVNSSAFLNDD150 pKa = 3.34ANSGRR155 pKa = 11.84FKK157 pKa = 10.58ILARR161 pKa = 11.84RR162 pKa = 11.84SYY164 pKa = 11.39SLTGNVATAGQQNDD178 pKa = 3.7STFQMIDD185 pKa = 3.24EE186 pKa = 4.23YY187 pKa = 11.79VKK189 pKa = 10.94LKK191 pKa = 10.58NLPSVFKK198 pKa = 10.79AVGSGDD204 pKa = 3.08IGDD207 pKa = 4.04IEE209 pKa = 4.47EE210 pKa = 4.36GALYY214 pKa = 10.39LVTVGTTPTGTADD227 pKa = 4.53MIFQLAARR235 pKa = 11.84TRR237 pKa = 11.84FLDD240 pKa = 3.39VV241 pKa = 3.29

Molecular weight: 26.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

600

241

359

300.0

33.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.167 ± 1.235

1.333 ± 0.839

5.0 ± 0.274

2.667 ± 0.634

4.0 ± 0.69

7.5 ± 1.809

2.667 ± 1.679

4.5 ± 0.04

5.0 ± 0.013

7.667 ± 0.398

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.621

5.333 ± 0.038

6.5 ± 1.48

5.333 ± 0.223

6.833 ± 0.139

5.167 ± 0.666

7.333 ± 1.13

6.833 ± 0.923

2.0 ± 0.737

4.833 ± 0.431
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski