Klebsiella phage KP8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Kaypoctavirus; Klebsiella virus KP8

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1CDA5|A0A2P1CDA5_9CAUD RIIA-like protein OS=Klebsiella phage KP8 OX=2099850 PE=4 SV=1
MM1 pKa = 7.36EE2 pKa = 6.5RR3 pKa = 11.84YY4 pKa = 7.92TPDD7 pKa = 3.21STHH10 pKa = 7.09DD11 pKa = 3.57ALVIMGNPRR20 pKa = 11.84ALAYY24 pKa = 9.92QWIVEE29 pKa = 4.15YY30 pKa = 10.63AKK32 pKa = 10.83YY33 pKa = 10.13ISPEE37 pKa = 3.95KK38 pKa = 10.95EE39 pKa = 3.82EE40 pKa = 5.37DD41 pKa = 3.36DD42 pKa = 3.62SDD44 pKa = 4.98YY45 pKa = 11.65YY46 pKa = 11.4DD47 pKa = 4.5LSAVDD52 pKa = 3.98AEE54 pKa = 4.44EE55 pKa = 5.05LIEE58 pKa = 4.86AALSHH63 pKa = 6.64QDD65 pKa = 3.73GGWGEE70 pKa = 4.21YY71 pKa = 8.91ICRR74 pKa = 11.84GGVYY78 pKa = 10.1EE79 pKa = 5.38GFQLDD84 pKa = 3.57STFWDD89 pKa = 3.19KK90 pKa = 10.82FAIIMGIEE98 pKa = 3.93RR99 pKa = 11.84EE100 pKa = 4.41SVEE103 pKa = 3.69EE104 pKa = 4.37RR105 pKa = 11.84SFFTCSCC112 pKa = 3.99

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1CCG1|A0A2P1CCG1_9CAUD Putative helical domain containing protein OS=Klebsiella phage KP8 OX=2099850 PE=4 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84TLCNRR7 pKa = 11.84FNPQKK12 pKa = 10.38LITMTSLYY20 pKa = 10.2GSGMGFAKK28 pKa = 10.14RR29 pKa = 11.84WQVSVSDD36 pKa = 3.98DD37 pKa = 3.11RR38 pKa = 11.84GRR40 pKa = 11.84CYY42 pKa = 11.23DD43 pKa = 3.56HH44 pKa = 6.95YY45 pKa = 11.13FVAKK49 pKa = 7.5PTRR52 pKa = 11.84KK53 pKa = 9.61QIRR56 pKa = 11.84KK57 pKa = 8.25LHH59 pKa = 6.1NIGAKK64 pKa = 9.63KK65 pKa = 10.37

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

22862

31

3474

235.7

26.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.503 ± 0.538

0.905 ± 0.167

5.892 ± 0.15

6.487 ± 0.27

3.512 ± 0.156

6.649 ± 0.192

1.833 ± 0.162

5.752 ± 0.226

6.426 ± 0.292

8.166 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.009 ± 0.166

5.437 ± 0.227

4.12 ± 0.19

4.099 ± 0.269

4.47 ± 0.164

5.953 ± 0.26

6.605 ± 0.248

7.06 ± 0.206

1.286 ± 0.117

3.836 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski