Pseudomonas sp. S-6-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3645 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0B7J6|A0A1V0B7J6_9PSED HTH araC/xylS-type domain-containing protein OS=Pseudomonas sp. S-6-2 OX=1931241 GN=BVH74_14600 PE=4 SV=1
MM1 pKa = 6.88KK2 pKa = 8.92TQRR5 pKa = 11.84VLTPLAFGLAVAISGSAYY23 pKa = 10.46AVGDD27 pKa = 3.57GASAVIYY34 pKa = 7.55DD35 pKa = 3.77TQKK38 pKa = 10.62NFNNEE43 pKa = 3.73VLNQGTEE50 pKa = 3.9NTASVNDD57 pKa = 4.1SLNSASGNVGVNVAAGDD74 pKa = 3.85NNQQANAAAVATADD88 pKa = 3.41ALFVFGFPVVASAQASIDD106 pKa = 3.79VQQKK110 pKa = 10.5AFNNDD115 pKa = 2.83LTNYY119 pKa = 9.41SVPNDD124 pKa = 3.23ASLNNSANDD133 pKa = 3.42TSGNVGINIAAGNYY147 pKa = 7.18NQQKK151 pKa = 9.92NDD153 pKa = 3.32MAIASSSSAFTAGASVNVEE172 pKa = 3.66QKK174 pKa = 11.04SFGNNTDD181 pKa = 3.19NLAVLDD187 pKa = 3.92YY188 pKa = 10.74GKK190 pKa = 10.49QYY192 pKa = 11.28VHH194 pKa = 7.05LKK196 pKa = 10.38LKK198 pKa = 10.82ADD200 pKa = 3.59GTYY203 pKa = 10.58EE204 pKa = 4.16GVSDD208 pKa = 4.7QIGDD212 pKa = 3.55VYY214 pKa = 11.25LDD216 pKa = 3.32VWEE219 pKa = 5.51GPTHH223 pKa = 6.83PGGANIGHH231 pKa = 7.14IDD233 pKa = 3.6VDD235 pKa = 4.3SAAQGAVDD243 pKa = 4.58LNGDD247 pKa = 3.84GGAFAFNEE255 pKa = 4.21EE256 pKa = 4.18GTLGLKK262 pKa = 10.62GSVSGYY268 pKa = 8.98IPVVVGFTGPVTNNATINGSLNNVSGNVGVNVAAGGGNQQSNSLAIAAGCTACPNGGGGGGEE330 pKa = 4.08LL331 pKa = 4.22

Molecular weight: 33.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0B2E8|A0A1V0B2E8_9PSED Uncharacterized protein OS=Pseudomonas sp. S-6-2 OX=1931241 GN=BVH74_04655 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3645

0

3645

1217832

23

3849

334.1

36.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.757 ± 0.045

0.999 ± 0.013

5.399 ± 0.031

6.056 ± 0.038

3.489 ± 0.023

7.931 ± 0.045

2.289 ± 0.022

4.854 ± 0.031

2.902 ± 0.035

12.03 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.019

3.013 ± 0.033

4.849 ± 0.032

5.005 ± 0.037

6.817 ± 0.042

5.723 ± 0.033

4.576 ± 0.027

6.956 ± 0.037

1.472 ± 0.02

2.505 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski