Escherichia phage K30

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; Escherichia virus K30

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8R4P8|F8R4P8_9CAUD Uncharacterized protein OS=Escherichia phage K30 OX=1041524 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.17YY9 pKa = 10.19EE10 pKa = 4.4LLAATVEE17 pKa = 4.25AFNEE21 pKa = 4.23HH22 pKa = 5.39IQYY25 pKa = 11.0DD26 pKa = 4.4KK27 pKa = 10.92LTEE30 pKa = 4.66DD31 pKa = 4.75DD32 pKa = 4.06DD33 pKa = 4.39WYY35 pKa = 10.75DD36 pKa = 3.48ALHH39 pKa = 6.21EE40 pKa = 4.53VVDD43 pKa = 3.87SHH45 pKa = 7.03VPHH48 pKa = 6.94YY49 pKa = 9.42YY50 pKa = 10.33HH51 pKa = 7.85EE52 pKa = 4.82IFTVMAADD60 pKa = 5.45GIDD63 pKa = 3.71HH64 pKa = 6.84EE65 pKa = 5.51FDD67 pKa = 4.8DD68 pKa = 5.63SDD70 pKa = 6.08LIPDD74 pKa = 3.86TKK76 pKa = 10.67DD77 pKa = 3.0VSRR80 pKa = 11.84ICQARR85 pKa = 11.84IYY87 pKa = 9.29EE88 pKa = 4.11ALYY91 pKa = 11.04NDD93 pKa = 3.97ILNDD97 pKa = 3.93SRR99 pKa = 11.84VIWCEE104 pKa = 3.42QGEE107 pKa = 4.24DD108 pKa = 5.12DD109 pKa = 6.42EE110 pKa = 6.0EE111 pKa = 6.49DD112 pKa = 4.39DD113 pKa = 5.09YY114 pKa = 12.26NDD116 pKa = 3.26

Molecular weight: 13.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8R4Q1|F8R4Q1_9CAUD Protein kinase OS=Escherichia phage K30 OX=1041524 PE=4 SV=1
MM1 pKa = 7.17SQHH4 pKa = 6.13TNLKK8 pKa = 9.87KK9 pKa = 10.2AFKK12 pKa = 8.68IARR15 pKa = 11.84MAMAYY20 pKa = 10.05GAGEE24 pKa = 4.09RR25 pKa = 11.84RR26 pKa = 11.84TKK28 pKa = 10.58RR29 pKa = 11.84ILAAGAGRR37 pKa = 11.84LPARR41 pKa = 11.84QRR43 pKa = 11.84KK44 pKa = 6.94WVMQQVYY51 pKa = 8.68TEE53 pKa = 5.09LYY55 pKa = 8.49HH56 pKa = 5.94PQRR59 pKa = 11.84IPDD62 pKa = 3.63DD63 pKa = 4.92CIWSSVGWKK72 pKa = 9.76RR73 pKa = 11.84VVINN77 pKa = 4.11

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12649

38

1321

258.1

28.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.862 ± 0.475

0.98 ± 0.16

6.443 ± 0.198

6.712 ± 0.427

3.732 ± 0.173

7.898 ± 0.413

1.81 ± 0.192

4.799 ± 0.137

6.601 ± 0.331

8.103 ± 0.411

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.625 ± 0.172

4.293 ± 0.243

3.755 ± 0.168

4.301 ± 0.307

5.487 ± 0.194

6.151 ± 0.302

5.629 ± 0.324

6.862 ± 0.363

1.447 ± 0.183

3.51 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski