Microvirga vignae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H1R4K9|A0A0H1R4K9_9RHIZ Uncharacterized protein OS=Microvirga vignae OX=1225564 GN=AA309_28010 PE=4 SV=1
MM1 pKa = 7.59SINWLVTVQSPYY13 pKa = 11.1NIIIPTYY20 pKa = 10.47GEE22 pKa = 4.02YY23 pKa = 10.87GGPNYY28 pKa = 10.56SDD30 pKa = 3.21GHH32 pKa = 5.04VLQPGEE38 pKa = 4.39VPPLTTTPADD48 pKa = 4.37DD49 pKa = 5.11LDD51 pKa = 4.2ALYY54 pKa = 10.24RR55 pKa = 11.84AHH57 pKa = 6.71DD58 pKa = 3.54QVYY61 pKa = 7.87RR62 pKa = 11.84TSSDD66 pKa = 3.54PLVLAQADD74 pKa = 3.86LQLIRR79 pKa = 11.84GIAQLSDD86 pKa = 3.01AQLSVEE92 pKa = 3.79AHH94 pKa = 6.8LYY96 pKa = 10.71AGATQLALIDD106 pKa = 4.03QIKK109 pKa = 9.86NRR111 pKa = 11.84YY112 pKa = 6.79GHH114 pKa = 7.37PEE116 pKa = 3.7LFLPGEE122 pKa = 4.28EE123 pKa = 4.52ALLAQDD129 pKa = 5.63ALDD132 pKa = 3.99NLQKK136 pKa = 11.06GGINPEE142 pKa = 4.12PNEE145 pKa = 3.61IAAALDD151 pKa = 4.0LYY153 pKa = 11.61DD154 pKa = 4.8NLLEE158 pKa = 4.19YY159 pKa = 10.79AQGTLAGINLRR170 pKa = 11.84DD171 pKa = 3.83LDD173 pKa = 4.06ILVDD177 pKa = 3.75DD178 pKa = 4.35VFYY181 pKa = 11.03FF182 pKa = 4.0

Molecular weight: 19.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H1RGT2|A0A0H1RGT2_9RHIZ X-Pro dipeptidase OS=Microvirga vignae OX=1225564 GN=AA309_06270 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84KK29 pKa = 9.21VIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.11GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5672

0

5672

1617832

34

3758

285.2

31.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.689 ± 0.04

0.835 ± 0.011

5.363 ± 0.026

5.996 ± 0.034

3.721 ± 0.022

8.298 ± 0.031

2.095 ± 0.015

5.49 ± 0.022

3.469 ± 0.03

10.234 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.438 ± 0.014

2.618 ± 0.018

5.224 ± 0.027

3.298 ± 0.02

7.2 ± 0.038

5.612 ± 0.022

5.35 ± 0.027

7.502 ± 0.027

1.326 ± 0.013

2.242 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski