Scytalidium sp. 3C

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Scytalidium; unclassified Scytalidium

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A425BTE8|A0A425BTE8_9PEZI Leukotriene A-4 hydrolase homolog OS=Scytalidium sp. 3C OX=2487125 GN=DH86_00001357 PE=3 SV=1
MM1 pKa = 7.23IQGGTQNTVISAIPAAIAQEE21 pKa = 4.17TSLLLGLWASAGQDD35 pKa = 3.36DD36 pKa = 5.14FNNEE40 pKa = 3.17IQALKK45 pKa = 9.4TAIQQFGSQLAPLLVGISVGSEE67 pKa = 3.32DD68 pKa = 5.2LYY70 pKa = 11.01RR71 pKa = 11.84ISPTGVEE78 pKa = 4.26AKK80 pKa = 10.25SGAGANPDD88 pKa = 4.82DD89 pKa = 3.58IVNYY93 pKa = 9.96ISQVRR98 pKa = 11.84DD99 pKa = 3.88AIKK102 pKa = 9.28GTPLSGASIGHH113 pKa = 5.99VDD115 pKa = 3.37TWTAWVNGSNDD126 pKa = 3.41AVINACDD133 pKa = 3.96WIGVDD138 pKa = 4.82AYY140 pKa = 10.08PYY142 pKa = 9.68FQNTMSNGIDD152 pKa = 3.2QGASLFKK159 pKa = 10.25SAYY162 pKa = 10.08SATQQAANGKK172 pKa = 7.48PVWITEE178 pKa = 4.34TGWPVSGSTEE188 pKa = 3.98NLGVPSLQNAKK199 pKa = 9.59TYY201 pKa = 9.17WDD203 pKa = 3.51EE204 pKa = 4.28VGCDD208 pKa = 3.47FAFGKK213 pKa = 10.36INTWWYY219 pKa = 8.64TLQDD223 pKa = 3.99SQPTTPNPSFGVVGSTLSTTPLYY246 pKa = 10.78DD247 pKa = 4.16LSCSSSNSTSSSSSSASSASASATQSSSGASTTQGSGGVGAGSGSGSGSSATTSAAAGGSSSAPSSVAGGNASSAVAAASSGVATGGAGLSPNQANGNGVPPASTGNGTSSGSAAGGSTSGSGASNSTGVASGGSTNSTSSGSGSGSGSGSGSGTSTGSSGSSSSSPITSANSASLVSFPVMGSALVGFAAVVAALL443 pKa = 3.79

Molecular weight: 42.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A425BVE2|A0A425BVE2_9PEZI Chitin synthase (Fragment) OS=Scytalidium sp. 3C OX=2487125 GN=DH86_00002431 PE=3 SV=1
KKK2 pKa = 9.89RR3 pKa = 11.84CDDD6 pKa = 2.93KKK8 pKa = 8.82PCSRR12 pKa = 11.84CTRR15 pKa = 11.84LDDD18 pKa = 3.61DDD20 pKa = 3.9ARR22 pKa = 11.84LRR24 pKa = 11.84SHHH27 pKa = 6.57RR28 pKa = 11.84HHH30 pKa = 5.41RR31 pKa = 11.84PANNTSGQDDD41 pKa = 3.59EEE43 pKa = 4.54VEEE46 pKa = 4.21RR47 pKa = 11.84KKK49 pKa = 9.46KK50 pKa = 9.36YY51 pKa = 9.64AVTVRR56 pKa = 11.84RR57 pKa = 11.84DDD59 pKa = 3.17TGCDDD64 pKa = 2.67CKKK67 pKa = 10.37KK68 pKa = 10.37NVTRR72 pKa = 11.84KKK74 pKa = 9.11RR75 pKa = 11.84SAQIVEEE82 pKa = 3.93DD83 pKa = 4.3IYYY86 pKa = 10.12HH87 pKa = 5.32SEEE90 pKa = 4.33FVLPRR95 pKa = 11.84LRR97 pKa = 11.84SHHH100 pKa = 7.52PPLLYYY106 pKa = 10.22QHHH109 pKa = 7.0PPQMPSFRR117 pKa = 11.84SRR119 pKa = 11.84PNQTQLLCPKKK130 pKa = 10.01RR131 pKa = 11.84GPEEE135 pKa = 3.67AVLRR139 pKa = 11.84IQGFSSDDD147 pKa = 3.04HH148 pKa = 5.92LYYY151 pKa = 10.92NGFRR155 pKa = 11.84DDD157 pKa = 3.74DD158 pKa = 5.24LYYY161 pKa = 10.33HHH163 pKa = 7.18HH164 pKa = 7.41RR165 pKa = 11.84PQQISWSLLRR175 pKa = 11.84TQVMMMEEE183 pKa = 4.8VAHHH187 pKa = 6.28LLSGLYYY194 pKa = 9.04VNLLAKKK201 pKa = 9.63LANNRR206 pKa = 11.84IGYYY210 pKa = 10.07STP

Molecular weight: 24.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4330

0

4330

1786973

11

3850

412.7

45.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.675 ± 0.034

1.035 ± 0.013

5.554 ± 0.029

6.471 ± 0.041

3.688 ± 0.025

7.086 ± 0.036

2.186 ± 0.017

5.181 ± 0.027

5.345 ± 0.036

8.667 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.015

3.9 ± 0.022

5.966 ± 0.041

3.985 ± 0.03

5.683 ± 0.034

8.133 ± 0.048

5.968 ± 0.035

6.103 ± 0.031

1.334 ± 0.015

2.858 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski