Hathewaya histolytica (Clostridium histolyticum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Hathewaya

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2563 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U9RF56|A0A4U9RF56_HATHI TVP38/TMEM64 family membrane protein OS=Hathewaya histolytica OX=1498 GN=ydjZ PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.48SKK4 pKa = 10.51IIDD7 pKa = 3.75YY8 pKa = 11.2LNDD11 pKa = 3.35NDD13 pKa = 5.49LDD15 pKa = 4.59DD16 pKa = 4.08IKK18 pKa = 10.73EE19 pKa = 4.16IKK21 pKa = 10.56VSEE24 pKa = 4.06KK25 pKa = 10.91DD26 pKa = 3.64VFVLKK31 pKa = 10.69LNYY34 pKa = 10.35YY35 pKa = 9.9FDD37 pKa = 3.72SLEE40 pKa = 4.06IEE42 pKa = 4.56GANSYY47 pKa = 11.35AKK49 pKa = 10.21DD50 pKa = 3.25EE51 pKa = 4.39CEE53 pKa = 3.92EE54 pKa = 4.45GEE56 pKa = 4.18EE57 pKa = 4.38SIEE60 pKa = 4.31FYY62 pKa = 11.19EE63 pKa = 5.64DD64 pKa = 4.61FILPYY69 pKa = 10.54LSDD72 pKa = 3.56SAVDD76 pKa = 3.53NVEE79 pKa = 4.72DD80 pKa = 3.7IVRR83 pKa = 11.84DD84 pKa = 3.61VCEE87 pKa = 4.29EE88 pKa = 4.13LDD90 pKa = 5.04LEE92 pKa = 4.33YY93 pKa = 11.04QLISFDD99 pKa = 3.44IDD101 pKa = 3.69VEE103 pKa = 4.07EE104 pKa = 4.63SEE106 pKa = 4.7YY107 pKa = 10.25NTFIIAFYY115 pKa = 10.73EE116 pKa = 3.89RR117 pKa = 11.84DD118 pKa = 3.49KK119 pKa = 11.73EE120 pKa = 4.39FDD122 pKa = 3.49LEE124 pKa = 4.6DD125 pKa = 4.63YY126 pKa = 9.45ITEE129 pKa = 4.71III131 pKa = 4.12

Molecular weight: 15.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V6KF50|A0A4V6KF50_HATHI Uncharacterized protein OS=Hathewaya histolytica OX=1498 GN=NCTC503_01458 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.89KK9 pKa = 7.86RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.09GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MSTLSGRR28 pKa = 11.84NILKK32 pKa = 10.18KK33 pKa = 10.15RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.28GRR39 pKa = 11.84KK40 pKa = 8.94RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2563

0

2563

768538

30

2871

299.9

33.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.036 ± 0.053

1.186 ± 0.023

5.304 ± 0.036

7.77 ± 0.053

4.419 ± 0.041

6.398 ± 0.05

1.389 ± 0.019

10.151 ± 0.061

9.796 ± 0.054

9.193 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.026

6.392 ± 0.052

2.713 ± 0.023

2.147 ± 0.021

3.423 ± 0.028

6.377 ± 0.038

4.73 ± 0.03

6.284 ± 0.04

0.623 ± 0.015

4.092 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski