Amino acid dipepetide frequency for Methanobacterium lacus (strain AL-21)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.868AlaAla: 4.868 ± 0.1
0.728AlaCys: 0.728 ± 0.042
3.395AlaAsp: 3.395 ± 0.095
3.841AlaGlu: 3.841 ± 0.082
2.386AlaPhe: 2.386 ± 0.063
4.502AlaGly: 4.502 ± 0.097
0.996AlaHis: 0.996 ± 0.044
5.467AlaIle: 5.467 ± 0.113
3.877AlaLys: 3.877 ± 0.084
6.057AlaLeu: 6.057 ± 0.132
1.756AlaMet: 1.756 ± 0.049
2.704AlaAsn: 2.704 ± 0.08
1.822AlaPro: 1.822 ± 0.059
1.496AlaGln: 1.496 ± 0.052
2.106AlaArg: 2.106 ± 0.059
3.851AlaSer: 3.851 ± 0.082
3.251AlaThr: 3.251 ± 0.098
5.361AlaVal: 5.361 ± 0.102
0.411AlaTrp: 0.411 ± 0.021
1.86AlaTyr: 1.86 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.629CysAla: 0.629 ± 0.032
0.17CysCys: 0.17 ± 0.017
0.597CysAsp: 0.597 ± 0.033
0.734CysGlu: 0.734 ± 0.038
0.404CysPhe: 0.404 ± 0.023
1.312CysGly: 1.312 ± 0.059
0.232CysHis: 0.232 ± 0.021
0.768CysIle: 0.768 ± 0.033
0.701CysLys: 0.701 ± 0.035
0.84CysLeu: 0.84 ± 0.043
0.3CysMet: 0.3 ± 0.023
0.546CysAsn: 0.546 ± 0.028
0.798CysPro: 0.798 ± 0.055
0.264CysGln: 0.264 ± 0.019
0.407CysArg: 0.407 ± 0.025
0.749CysSer: 0.749 ± 0.032
0.573CysThr: 0.573 ± 0.034
0.745CysVal: 0.745 ± 0.034
0.059CysTrp: 0.059 ± 0.008
0.426CysTyr: 0.426 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
3.277AspAla: 3.277 ± 0.076
0.538AspCys: 0.538 ± 0.026
3.116AspAsp: 3.116 ± 0.104
4.58AspGlu: 4.58 ± 0.097
2.349AspPhe: 2.349 ± 0.062
3.672AspGly: 3.672 ± 0.094
1.093AspHis: 1.093 ± 0.042
5.096AspIle: 5.096 ± 0.088
3.746AspLys: 3.746 ± 0.087
5.655AspLeu: 5.655 ± 0.1
1.717AspMet: 1.717 ± 0.048
2.469AspAsn: 2.469 ± 0.065
2.68AspPro: 2.68 ± 0.072
1.276AspGln: 1.276 ± 0.045
1.714AspArg: 1.714 ± 0.054
3.193AspSer: 3.193 ± 0.1
2.542AspThr: 2.542 ± 0.074
4.629AspVal: 4.629 ± 0.089
0.428AspTrp: 0.428 ± 0.03
2.241AspTyr: 2.241 ± 0.071
0.0AspXaa: 0.0 ± 0.0
Glu
3.998GluAla: 3.998 ± 0.089
0.71GluCys: 0.71 ± 0.038
4.357GluAsp: 4.357 ± 0.092
5.677GluGlu: 5.677 ± 0.127
2.782GluPhe: 2.782 ± 0.067
3.925GluGly: 3.925 ± 0.085
1.13GluHis: 1.13 ± 0.044
6.64GluIle: 6.64 ± 0.129
5.867GluLys: 5.867 ± 0.112
6.817GluLeu: 6.817 ± 0.137
2.055GluMet: 2.055 ± 0.062
4.463GluAsn: 4.463 ± 0.096
1.914GluPro: 1.914 ± 0.063
1.488GluGln: 1.488 ± 0.048
2.603GluArg: 2.603 ± 0.071
3.946GluSer: 3.946 ± 0.085
3.968GluThr: 3.968 ± 0.096
4.492GluVal: 4.492 ± 0.101
0.486GluTrp: 0.486 ± 0.026
2.244GluTyr: 2.244 ± 0.063
0.0GluXaa: 0.0 ± 0.0
Phe
2.479PheAla: 2.479 ± 0.065
0.482PheCys: 0.482 ± 0.028
2.328PheAsp: 2.328 ± 0.06
2.686PheGlu: 2.686 ± 0.061
1.805PhePhe: 1.805 ± 0.061
3.05PheGly: 3.05 ± 0.082
0.658PheHis: 0.658 ± 0.034
3.666PheIle: 3.666 ± 0.087
3.179PheLys: 3.179 ± 0.061
3.943PheLeu: 3.943 ± 0.087
1.204PheMet: 1.204 ± 0.044
2.403PheAsn: 2.403 ± 0.06
1.364PhePro: 1.364 ± 0.043
0.992PheGln: 0.992 ± 0.042
1.33PheArg: 1.33 ± 0.045
2.888PheSer: 2.888 ± 0.065
2.349PheThr: 2.349 ± 0.071
2.8PheVal: 2.8 ± 0.068
0.344PheTrp: 0.344 ± 0.022
1.496PheTyr: 1.496 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
4.661GlyAla: 4.661 ± 0.122
0.929GlyCys: 0.929 ± 0.042
3.647GlyAsp: 3.647 ± 0.079
3.826GlyGlu: 3.826 ± 0.078
3.256GlyPhe: 3.256 ± 0.066
4.783GlyGly: 4.783 ± 0.132
1.216GlyHis: 1.216 ± 0.046
6.786GlyIle: 6.786 ± 0.119
4.953GlyLys: 4.953 ± 0.088
6.138GlyLeu: 6.138 ± 0.129
1.992GlyMet: 1.992 ± 0.054
3.711GlyAsn: 3.711 ± 0.116
1.983GlyPro: 1.983 ± 0.059
1.561GlyGln: 1.561 ± 0.045
2.324GlyArg: 2.324 ± 0.059
4.756GlySer: 4.756 ± 0.089
4.667GlyThr: 4.667 ± 0.143
5.283GlyVal: 5.283 ± 0.111
0.662GlyTrp: 0.662 ± 0.029
2.824GlyTyr: 2.824 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
0.917HisAla: 0.917 ± 0.043
0.233HisCys: 0.233 ± 0.016
1.045HisAsp: 1.045 ± 0.036
1.276HisGlu: 1.276 ± 0.051
0.64HisPhe: 0.64 ± 0.029
1.399HisGly: 1.399 ± 0.048
0.457HisHis: 0.457 ± 0.025
1.266HisIle: 1.266 ± 0.041
1.016HisLys: 1.016 ± 0.042
1.401HisLeu: 1.401 ± 0.042
0.419HisMet: 0.419 ± 0.025
0.775HisAsn: 0.775 ± 0.032
0.929HisPro: 0.929 ± 0.04
0.439HisGln: 0.439 ± 0.025
0.657HisArg: 0.657 ± 0.032
1.133HisSer: 1.133 ± 0.037
0.844HisThr: 0.844 ± 0.038
1.277HisVal: 1.277 ± 0.042
0.126HisTrp: 0.126 ± 0.014
0.664HisTyr: 0.664 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
5.414IleAla: 5.414 ± 0.102
0.972IleCys: 0.972 ± 0.036
4.521IleAsp: 4.521 ± 0.073
5.934IleGlu: 5.934 ± 0.12
4.125IlePhe: 4.125 ± 0.105
6.124IleGly: 6.124 ± 0.122
1.513IleHis: 1.513 ± 0.05
8.309IleIle: 8.309 ± 0.152
6.68IleLys: 6.68 ± 0.091
8.529IleLeu: 8.529 ± 0.131
2.326IleMet: 2.326 ± 0.067
4.928IleAsn: 4.928 ± 0.103
3.585IlePro: 3.585 ± 0.082
2.21IleGln: 2.21 ± 0.059
2.785IleArg: 2.785 ± 0.066
6.042IleSer: 6.042 ± 0.103
5.22IleThr: 5.22 ± 0.102
5.896IleVal: 5.896 ± 0.101
0.682IleTrp: 0.682 ± 0.035
3.348IleTyr: 3.348 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
3.792LysAla: 3.792 ± 0.081
0.903LysCys: 0.903 ± 0.05
4.312LysAsp: 4.312 ± 0.102
5.596LysGlu: 5.596 ± 0.12
2.979LysPhe: 2.979 ± 0.07
4.147LysGly: 4.147 ± 0.085
1.252LysHis: 1.252 ± 0.044
7.018LysIle: 7.018 ± 0.117
6.248LysLys: 6.248 ± 0.122
6.552LysLeu: 6.552 ± 0.112
2.159LysMet: 2.159 ± 0.054
5.174LysAsn: 5.174 ± 0.088
2.688LysPro: 2.688 ± 0.067
1.896LysGln: 1.896 ± 0.05
2.779LysArg: 2.779 ± 0.07
4.484LysSer: 4.484 ± 0.09
4.934LysThr: 4.934 ± 0.08
4.39LysVal: 4.39 ± 0.074
0.594LysTrp: 0.594 ± 0.034
2.933LysTyr: 2.933 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
5.512LeuAla: 5.512 ± 0.1
0.915LeuCys: 0.915 ± 0.043
5.139LeuAsp: 5.139 ± 0.096
6.636LeuGlu: 6.636 ± 0.135
3.703LeuPhe: 3.703 ± 0.094
6.184LeuGly: 6.184 ± 0.103
1.291LeuHis: 1.291 ± 0.042
8.322LeuIle: 8.322 ± 0.161
8.349LeuLys: 8.349 ± 0.128
7.959LeuLeu: 7.959 ± 0.146
2.544LeuMet: 2.544 ± 0.064
5.666LeuAsn: 5.666 ± 0.11
3.14LeuPro: 3.14 ± 0.066
2.096LeuGln: 2.096 ± 0.058
3.071LeuArg: 3.071 ± 0.069
5.842LeuSer: 5.842 ± 0.098
5.05LeuThr: 5.05 ± 0.084
6.11LeuVal: 6.11 ± 0.098
0.676LeuTrp: 0.676 ± 0.03
2.768LeuTyr: 2.768 ± 0.062
0.0LeuXaa: 0.0 ± 0.0
Met
2.001MetAla: 2.001 ± 0.055
0.246MetCys: 0.246 ± 0.02
2.044MetAsp: 2.044 ± 0.053
2.261MetGlu: 2.261 ± 0.06
0.964MetPhe: 0.964 ± 0.035
2.28MetGly: 2.28 ± 0.057
0.429MetHis: 0.429 ± 0.027
2.373MetIle: 2.373 ± 0.058
2.244MetLys: 2.244 ± 0.058
2.127MetLeu: 2.127 ± 0.054
0.756MetMet: 0.756 ± 0.036
1.573MetAsn: 1.573 ± 0.044
0.896MetPro: 0.896 ± 0.036
0.606MetGln: 0.606 ± 0.029
0.865MetArg: 0.865 ± 0.033
1.58MetSer: 1.58 ± 0.052
1.285MetThr: 1.285 ± 0.046
2.331MetVal: 2.331 ± 0.059
0.163MetTrp: 0.163 ± 0.015
0.671MetTyr: 0.671 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.014AsnAla: 3.014 ± 0.076
0.647AsnCys: 0.647 ± 0.038
2.584AsnAsp: 2.584 ± 0.063
3.314AsnGlu: 3.314 ± 0.082
2.373AsnPhe: 2.373 ± 0.063
4.143AsnGly: 4.143 ± 0.136
1.037AsnHis: 1.037 ± 0.036
5.121AsnIle: 5.121 ± 0.1
3.894AsnLys: 3.894 ± 0.078
5.147AsnLeu: 5.147 ± 0.1
1.589AsnMet: 1.589 ± 0.05
3.923AsnAsn: 3.923 ± 0.188
2.656AsnPro: 2.656 ± 0.074
1.89AsnGln: 1.89 ± 0.06
1.811AsnArg: 1.811 ± 0.067
4.39AsnSer: 4.39 ± 0.124
3.328AsnThr: 3.328 ± 0.119
3.682AsnVal: 3.682 ± 0.071
0.584AsnTrp: 0.584 ± 0.032
2.392AsnTyr: 2.392 ± 0.069
0.0AsnXaa: 0.0 ± 0.0
Pro
1.941ProAla: 1.941 ± 0.059
0.43ProCys: 0.43 ± 0.027
2.421ProAsp: 2.421 ± 0.061
3.433ProGlu: 3.433 ± 0.088
1.576ProPhe: 1.576 ± 0.048
2.366ProGly: 2.366 ± 0.074
0.676ProHis: 0.676 ± 0.033
2.835ProIle: 2.835 ± 0.06
2.437ProLys: 2.437 ± 0.067
3.235ProLeu: 3.235 ± 0.07
0.953ProMet: 0.953 ± 0.038
1.786ProAsn: 1.786 ± 0.049
1.07ProPro: 1.07 ± 0.038
1.037ProGln: 1.037 ± 0.043
1.118ProArg: 1.118 ± 0.043
2.273ProSer: 2.273 ± 0.056
2.053ProThr: 2.053 ± 0.068
3.077ProVal: 3.077 ± 0.08
0.295ProTrp: 0.295 ± 0.022
1.301ProTyr: 1.301 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
1.442GlnAla: 1.442 ± 0.047
0.228GlnCys: 0.228 ± 0.021
1.285GlnAsp: 1.285 ± 0.05
1.643GlnGlu: 1.643 ± 0.058
0.986GlnPhe: 0.986 ± 0.034
1.513GlnGly: 1.513 ± 0.045
0.437GlnHis: 0.437 ± 0.027
2.373GlnIle: 2.373 ± 0.055
2.047GlnLys: 2.047 ± 0.057
2.233GlnLeu: 2.233 ± 0.065
0.753GlnMet: 0.753 ± 0.03
1.698GlnAsn: 1.698 ± 0.055
0.738GlnPro: 0.738 ± 0.032
0.673GlnGln: 0.673 ± 0.031
1.049GlnArg: 1.049 ± 0.042
1.547GlnSer: 1.547 ± 0.043
1.664GlnThr: 1.664 ± 0.045
1.488GlnVal: 1.488 ± 0.046
0.189GlnTrp: 0.189 ± 0.016
0.887GlnTyr: 0.887 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
2.082ArgAla: 2.082 ± 0.056
0.428ArgCys: 0.428 ± 0.025
1.9ArgAsp: 1.9 ± 0.057
2.711ArgGlu: 2.711 ± 0.063
1.373ArgPhe: 1.373 ± 0.047
2.216ArgGly: 2.216 ± 0.06
0.456ArgHis: 0.456 ± 0.024
3.102ArgIle: 3.102 ± 0.066
2.736ArgLys: 2.736 ± 0.072
2.706ArgLeu: 2.706 ± 0.066
1.084ArgMet: 1.084 ± 0.038
1.835ArgAsn: 1.835 ± 0.056
0.957ArgPro: 0.957 ± 0.046
0.77ArgGln: 0.77 ± 0.036
1.569ArgArg: 1.569 ± 0.057
2.266ArgSer: 2.266 ± 0.059
1.74ArgThr: 1.74 ± 0.044
2.361ArgVal: 2.361 ± 0.071
0.263ArgTrp: 0.263 ± 0.023
1.276ArgTyr: 1.276 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
3.689SerAla: 3.689 ± 0.077
0.725SerCys: 0.725 ± 0.039
3.344SerAsp: 3.344 ± 0.08
4.252SerGlu: 4.252 ± 0.098
2.789SerPhe: 2.789 ± 0.073
5.066SerGly: 5.066 ± 0.122
1.042SerHis: 1.042 ± 0.039
5.624SerIle: 5.624 ± 0.101
5.083SerLys: 5.083 ± 0.095
5.733SerLeu: 5.733 ± 0.103
1.699SerMet: 1.699 ± 0.049
3.856SerAsn: 3.856 ± 0.122
2.259SerPro: 2.259 ± 0.054
1.815SerGln: 1.815 ± 0.05
2.243SerArg: 2.243 ± 0.056
4.864SerSer: 4.864 ± 0.114
3.996SerThr: 3.996 ± 0.091
4.26SerVal: 4.26 ± 0.094
0.567SerTrp: 0.567 ± 0.028
2.563SerTyr: 2.563 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
4.02ThrAla: 4.02 ± 0.105
0.625ThrCys: 0.625 ± 0.036
3.135ThrAsp: 3.135 ± 0.072
3.365ThrGlu: 3.365 ± 0.068
2.191ThrPhe: 2.191 ± 0.061
4.843ThrGly: 4.843 ± 0.114
0.993ThrHis: 0.993 ± 0.037
5.146ThrIle: 5.146 ± 0.115
3.532ThrLys: 3.532 ± 0.072
5.092ThrLeu: 5.092 ± 0.106
1.389ThrMet: 1.389 ± 0.042
3.17ThrAsn: 3.17 ± 0.108
2.391ThrPro: 2.391 ± 0.074
1.452ThrGln: 1.452 ± 0.051
1.695ThrArg: 1.695 ± 0.051
3.905ThrSer: 3.905 ± 0.108
3.637ThrThr: 3.637 ± 0.109
4.897ThrVal: 4.897 ± 0.125
0.4ThrTrp: 0.4 ± 0.023
1.885ThrTyr: 1.885 ± 0.071
0.0ThrXaa: 0.0 ± 0.0
Val
4.755ValAla: 4.755 ± 0.086
0.833ValCys: 0.833 ± 0.04
4.378ValAsp: 4.378 ± 0.097
5.255ValGlu: 5.255 ± 0.101
2.771ValPhe: 2.771 ± 0.076
5.09ValGly: 5.09 ± 0.097
1.196ValHis: 1.196 ± 0.038
5.541ValIle: 5.541 ± 0.111
5.226ValLys: 5.226 ± 0.096
6.684ValLeu: 6.684 ± 0.106
1.797ValMet: 1.797 ± 0.055
3.823ValAsn: 3.823 ± 0.088
2.755ValPro: 2.755 ± 0.067
1.615ValGln: 1.615 ± 0.054
2.169ValArg: 2.169 ± 0.065
4.758ValSer: 4.758 ± 0.095
4.239ValThr: 4.239 ± 0.132
5.887ValVal: 5.887 ± 0.114
0.524ValTrp: 0.524 ± 0.029
2.459ValTyr: 2.459 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
0.444TrpAla: 0.444 ± 0.024
0.077TrpCys: 0.077 ± 0.01
0.471TrpAsp: 0.471 ± 0.027
0.437TrpGlu: 0.437 ± 0.026
0.367TrpPhe: 0.367 ± 0.024
0.538TrpGly: 0.538 ± 0.03
0.126TrpHis: 0.126 ± 0.014
0.784TrpIle: 0.784 ± 0.038
0.613TrpLys: 0.613 ± 0.03
0.701TrpLeu: 0.701 ± 0.037
0.245TrpMet: 0.245 ± 0.018
0.616TrpAsn: 0.616 ± 0.031
0.207TrpPro: 0.207 ± 0.018
0.236TrpGln: 0.236 ± 0.017
0.267TrpArg: 0.267 ± 0.021
0.468TrpSer: 0.468 ± 0.029
0.404TrpThr: 0.404 ± 0.026
0.468TrpVal: 0.468 ± 0.026
0.162TrpTrp: 0.162 ± 0.017
0.312TrpTyr: 0.312 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.804TyrAla: 1.804 ± 0.05
0.435TyrCys: 0.435 ± 0.028
1.99TyrAsp: 1.99 ± 0.056
2.107TyrGlu: 2.107 ± 0.073
1.628TyrPhe: 1.628 ± 0.058
2.804TyrGly: 2.804 ± 0.068
0.682TyrHis: 0.682 ± 0.035
2.908TyrIle: 2.908 ± 0.069
2.402TyrLys: 2.402 ± 0.062
3.423TyrLeu: 3.423 ± 0.073
0.954TyrMet: 0.954 ± 0.037
2.399TyrAsn: 2.399 ± 0.085
1.48TyrPro: 1.48 ± 0.047
1.061TyrGln: 1.061 ± 0.037
1.27TyrArg: 1.27 ± 0.042
2.535TyrSer: 2.535 ± 0.071
2.019TyrThr: 2.019 ± 0.085
2.319TyrVal: 2.319 ± 0.06
0.33TyrTrp: 0.33 ± 0.024
1.469TyrTyr: 1.469 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2493 proteins (715694 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski