Clostridium sp. W14A

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3645 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C0BMT5|A0A1C0BMT5_9CLOT RNA polymerase subunit sigma-70 OS=Clostridium sp. W14A OX=1849176 GN=A7X67_04595 PE=4 SV=1
MM1 pKa = 7.62AANIYY6 pKa = 10.0ILSACDD12 pKa = 2.79AWAEE16 pKa = 4.01HH17 pKa = 5.96SSMRR21 pKa = 11.84ILGVTTDD28 pKa = 3.55EE29 pKa = 4.11NMLYY33 pKa = 11.23AMLAAKK39 pKa = 10.13IKK41 pKa = 10.56AGDD44 pKa = 3.65MEE46 pKa = 4.72YY47 pKa = 10.88DD48 pKa = 3.55GSGEE52 pKa = 4.07NAWSKK57 pKa = 9.63FQNDD61 pKa = 4.22FKK63 pKa = 11.57NGDD66 pKa = 3.36INFNKK71 pKa = 10.03LKK73 pKa = 10.59YY74 pKa = 10.6GFVQTYY80 pKa = 9.5EE81 pKa = 4.16DD82 pKa = 3.72MQITEE87 pKa = 5.39PISLAQFPEE96 pKa = 4.55AGEE99 pKa = 4.24VYY101 pKa = 10.77EE102 pKa = 5.18EE103 pKa = 3.76ITGAKK108 pKa = 9.09VRR110 pKa = 11.84ADD112 pKa = 3.7MEE114 pKa = 4.55RR115 pKa = 11.84LGLDD119 pKa = 3.11HH120 pKa = 7.41RR121 pKa = 11.84SLVYY125 pKa = 10.54SVVEE129 pKa = 3.77VHH131 pKa = 6.41TDD133 pKa = 2.98SGDD136 pKa = 3.22TSFYY140 pKa = 10.43MPGICDD146 pKa = 3.3RR147 pKa = 11.84DD148 pKa = 3.81SLEE151 pKa = 4.55EE152 pKa = 4.14NDD154 pKa = 5.71DD155 pKa = 4.02YY156 pKa = 11.94LDD158 pKa = 4.66LMDD161 pKa = 5.33GADD164 pKa = 3.75DD165 pKa = 4.17TEE167 pKa = 4.62VDD169 pKa = 3.9VSVSSYY175 pKa = 11.65SLGTGEE181 pKa = 5.23SEE183 pKa = 4.34YY184 pKa = 10.53PDD186 pKa = 3.71EE187 pKa = 4.41EE188 pKa = 4.27EE189 pKa = 3.95IAIIEE194 pKa = 4.3QYY196 pKa = 10.37TDD198 pKa = 3.63EE199 pKa = 5.4LDD201 pKa = 3.39EE202 pKa = 5.08EE203 pKa = 4.63YY204 pKa = 10.76GIDD207 pKa = 4.5PIQSDD212 pKa = 4.03SFSFEE217 pKa = 3.92YY218 pKa = 10.43EE219 pKa = 4.0AEE221 pKa = 4.23QEE223 pKa = 4.44CC224 pKa = 4.91

Molecular weight: 25.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C0BLY7|A0A1C0BLY7_9CLOT Carboxymuconolactone decarboxylase OS=Clostridium sp. W14A OX=1849176 GN=A7X67_01005 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.14RR3 pKa = 11.84KK4 pKa = 8.97HH5 pKa = 6.15KK6 pKa = 10.47KK7 pKa = 9.82YY8 pKa = 10.46LVEE11 pKa = 4.32FWKK14 pKa = 10.7IKK16 pKa = 10.28DD17 pKa = 3.6YY18 pKa = 11.85NEE20 pKa = 3.62IARR23 pKa = 11.84QIKK26 pKa = 10.11SAMQRR31 pKa = 11.84QSVSPLCPLHH41 pKa = 6.56TEE43 pKa = 3.92HH44 pKa = 6.54QPPYY48 pKa = 7.81TTEE51 pKa = 4.02FTAHH55 pKa = 6.4CRR57 pKa = 11.84NHH59 pKa = 4.79YY60 pKa = 9.33TSFFRR65 pKa = 11.84FCTEE69 pKa = 3.33GRR71 pKa = 11.84KK72 pKa = 8.37MVHH75 pKa = 5.76SLIARR80 pKa = 11.84APVYY84 pKa = 10.95GMTFFRR90 pKa = 11.84TGAFCFLAGNGCRR103 pKa = 11.84ASQRR107 pKa = 11.84VNSSAGTRR115 pKa = 11.84RR116 pKa = 11.84HH117 pKa = 4.67VPRR120 pKa = 11.84RR121 pKa = 11.84QNN123 pKa = 2.86

Molecular weight: 14.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3645

0

3645

1085271

37

1975

297.7

32.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.521 ± 0.044

1.592 ± 0.018

5.333 ± 0.031

6.441 ± 0.046

4.26 ± 0.032

7.648 ± 0.048

1.685 ± 0.017

6.508 ± 0.036

5.818 ± 0.035

9.643 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.712 ± 0.019

3.954 ± 0.024

3.959 ± 0.03

3.302 ± 0.023

5.38 ± 0.04

6.644 ± 0.037

5.342 ± 0.037

6.904 ± 0.029

0.888 ± 0.014

3.466 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski