Alkalibaculum bacchi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Alkalibaculum

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366IC42|A0A366IC42_9FIRM Putative alkaline shock family protein YloU OS=Alkalibaculum bacchi OX=645887 GN=DES36_10351 PE=3 SV=1
MM1 pKa = 7.4KK2 pKa = 10.26KK3 pKa = 10.42VIAFCMVVFMVFTLCACKK21 pKa = 10.15PKK23 pKa = 10.77GDD25 pKa = 3.89EE26 pKa = 4.39QKK28 pKa = 11.11DD29 pKa = 3.53KK30 pKa = 11.48QDD32 pKa = 3.46INEE35 pKa = 4.24SSDD38 pKa = 3.06GRR40 pKa = 11.84GDD42 pKa = 3.45VFDD45 pKa = 5.39DD46 pKa = 3.66YY47 pKa = 11.17KK48 pKa = 10.9IPEE51 pKa = 4.48GADD54 pKa = 3.61EE55 pKa = 3.9IRR57 pKa = 11.84QGVFYY62 pKa = 11.26YY63 pKa = 10.43EE64 pKa = 4.52LYY66 pKa = 10.37EE67 pKa = 4.34LSGDD71 pKa = 3.99PGDD74 pKa = 3.94GLTPRR79 pKa = 11.84EE80 pKa = 4.1GAGLVDD86 pKa = 4.61EE87 pKa = 5.38LLASTGLYY95 pKa = 10.27SGDD98 pKa = 3.71RR99 pKa = 11.84ISADD103 pKa = 2.98EE104 pKa = 4.14CLYY107 pKa = 10.66ISFDD111 pKa = 3.91DD112 pKa = 5.44LMIIDD117 pKa = 3.84SAMGRR122 pKa = 11.84EE123 pKa = 4.21CYY125 pKa = 9.72IYY127 pKa = 10.85SVALGTAEE135 pKa = 4.36GGLMGDD141 pKa = 4.86GYY143 pKa = 10.31QVIYY147 pKa = 10.11RR148 pKa = 11.84FSVDD152 pKa = 3.13YY153 pKa = 11.0SGNKK157 pKa = 7.31TASIYY162 pKa = 10.91EE163 pKa = 4.34DD164 pKa = 4.03FSDD167 pKa = 4.9TILDD171 pKa = 4.29DD172 pKa = 3.99GLGDD176 pKa = 4.84VISKK180 pKa = 11.05DD181 pKa = 3.34NTHH184 pKa = 6.81TDD186 pKa = 3.17NTSKK190 pKa = 11.15DD191 pKa = 3.56DD192 pKa = 3.54GRR194 pKa = 11.84GDD196 pKa = 4.1VLSQDD201 pKa = 4.08DD202 pKa = 4.54GKK204 pKa = 11.72GDD206 pKa = 5.23LIPYY210 pKa = 8.03GPNWRR215 pKa = 11.84GMYY218 pKa = 10.34VGDD221 pKa = 4.07EE222 pKa = 4.07FSIEE226 pKa = 3.61ITKK229 pKa = 10.48FNGSSFWFDD238 pKa = 2.46IYY240 pKa = 11.14LLRR243 pKa = 11.84NGSTVMSGTATLYY256 pKa = 10.49PDD258 pKa = 4.16DD259 pKa = 5.63KK260 pKa = 11.86YY261 pKa = 10.09MAEE264 pKa = 3.95YY265 pKa = 11.16GEE267 pKa = 4.77ISFSLYY273 pKa = 10.46KK274 pKa = 10.43DD275 pKa = 3.27YY276 pKa = 11.56SAIDD280 pKa = 3.51FFAPEE285 pKa = 4.08SSDD288 pKa = 3.12WAHH291 pKa = 6.28MRR293 pKa = 11.84GQYY296 pKa = 10.85KK297 pKa = 10.32FIEE300 pKa = 4.32

Molecular weight: 33.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366I3J4|A0A366I3J4_9FIRM Ribulose-phosphate 3-epimerase OS=Alkalibaculum bacchi OX=645887 GN=DES36_11420 PE=3 SV=1
MM1 pKa = 7.14KK2 pKa = 9.31QTYY5 pKa = 7.67QPKK8 pKa = 8.45KK9 pKa = 7.59RR10 pKa = 11.84KK11 pKa = 9.02RR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.41GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MASSTGQNVLSNRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.97GRR39 pKa = 11.84KK40 pKa = 8.84RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2857

0

2857

853553

29

2535

298.8

33.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.95 ± 0.051

1.134 ± 0.02

5.576 ± 0.033

7.611 ± 0.054

4.214 ± 0.039

6.592 ± 0.049

1.735 ± 0.021

9.437 ± 0.051

8.009 ± 0.046

9.247 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.756 ± 0.023

5.288 ± 0.036

3.102 ± 0.027

3.12 ± 0.024

3.675 ± 0.03

6.214 ± 0.036

5.083 ± 0.03

6.522 ± 0.035

0.704 ± 0.014

4.034 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski