Alces alces faeces associated microvirus MP21 4718

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJ12|A0A2Z5CJ12_9VIRU Major capsid protein OS=Alces alces faeces associated microvirus MP21 4718 OX=2219138 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.22RR3 pKa = 11.84RR4 pKa = 11.84NKK6 pKa = 10.02FSLSHH11 pKa = 5.95YY12 pKa = 10.94VLGTCKK18 pKa = 9.46MGYY21 pKa = 8.12MLPIGLTEE29 pKa = 4.26VLPGDD34 pKa = 4.89TIQQATSVFMRR45 pKa = 11.84LAPMLAPVMHH55 pKa = 6.71PVQVKK60 pKa = 7.93IHH62 pKa = 5.79HH63 pKa = 6.6WYY65 pKa = 9.86VPLRR69 pKa = 11.84LVFPKK74 pKa = 10.62FEE76 pKa = 4.52DD77 pKa = 5.01FITGGPDD84 pKa = 3.33GLDD87 pKa = 3.01TTEE90 pKa = 4.87FPTITSDD97 pKa = 3.28EE98 pKa = 4.17TDD100 pKa = 3.26GFLEE104 pKa = 5.04GSLADD109 pKa = 4.31HH110 pKa = 7.04LGLPPGIPGLTVSALPFRR128 pKa = 11.84AYY130 pKa = 11.12NLIYY134 pKa = 10.53NEE136 pKa = 4.15WYY138 pKa = 10.0RR139 pKa = 11.84DD140 pKa = 3.15QDD142 pKa = 3.96LEE144 pKa = 4.32QEE146 pKa = 4.31IAISDD151 pKa = 3.8EE152 pKa = 4.35PGADD156 pKa = 2.9TTTSIEE162 pKa = 4.21LLRR165 pKa = 11.84GDD167 pKa = 3.53WEE169 pKa = 4.0RR170 pKa = 11.84DD171 pKa = 3.29YY172 pKa = 10.99FTTARR177 pKa = 11.84PWPQKK182 pKa = 10.73GIDD185 pKa = 3.46VTVPVQASGTADD197 pKa = 3.35VNIDD201 pKa = 2.96ITGNGTPSFSKK212 pKa = 10.82VSGFASGALQTNSSDD227 pKa = 3.74TIVTFGASSSGVSGPTSLAWLNPALQASGTVTGLDD262 pKa = 3.17VGSVNINDD270 pKa = 3.46LRR272 pKa = 11.84EE273 pKa = 4.07AFALQRR279 pKa = 11.84FEE281 pKa = 4.25EE282 pKa = 4.21HH283 pKa = 6.19RR284 pKa = 11.84AMYY287 pKa = 9.64GSRR290 pKa = 11.84YY291 pKa = 9.33VEE293 pKa = 3.84YY294 pKa = 10.74LRR296 pKa = 11.84YY297 pKa = 10.0LGIKK301 pKa = 10.33ASDD304 pKa = 3.48ARR306 pKa = 11.84LQRR309 pKa = 11.84PEE311 pKa = 3.75YY312 pKa = 10.59LGGGKK317 pKa = 7.49QTIQFSEE324 pKa = 4.46VLQTAADD331 pKa = 3.72GDD333 pKa = 4.29NPVGTLRR340 pKa = 11.84GHH342 pKa = 7.31GIAAMRR348 pKa = 11.84SNRR351 pKa = 11.84YY352 pKa = 8.7RR353 pKa = 11.84RR354 pKa = 11.84FIEE357 pKa = 3.55EE358 pKa = 3.45HH359 pKa = 6.62GYY361 pKa = 10.13IISIMLIRR369 pKa = 11.84PKK371 pKa = 10.28SIYY374 pKa = 8.5QQGVPRR380 pKa = 11.84TWLRR384 pKa = 11.84RR385 pKa = 11.84VKK387 pKa = 10.61EE388 pKa = 4.3DD389 pKa = 3.16FWQQEE394 pKa = 4.18LQHH397 pKa = 6.83IGQQEE402 pKa = 4.43VLTQEE407 pKa = 4.33LFGAAEE413 pKa = 4.06PEE415 pKa = 4.49KK416 pKa = 11.1VFGYY420 pKa = 8.13QNRR423 pKa = 11.84YY424 pKa = 9.41DD425 pKa = 4.75DD426 pKa = 4.13YY427 pKa = 9.1RR428 pKa = 11.84QHH430 pKa = 6.56VSYY433 pKa = 11.02VAGEE437 pKa = 3.84FRR439 pKa = 11.84SILNYY444 pKa = 8.59WHH446 pKa = 7.75LARR449 pKa = 11.84EE450 pKa = 4.43FGNEE454 pKa = 3.6PVLNADD460 pKa = 5.4FISSVPTEE468 pKa = 4.48RR469 pKa = 11.84IFAAQEE475 pKa = 3.54NDD477 pKa = 3.16QIYY480 pKa = 10.85YY481 pKa = 8.12MANHH485 pKa = 7.06SIQARR490 pKa = 11.84RR491 pKa = 11.84LMAKK495 pKa = 9.66YY496 pKa = 10.23GNPII500 pKa = 3.34

Molecular weight: 56.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5CJ12|A0A2Z5CJ12_9VIRU Major capsid protein OS=Alces alces faeces associated microvirus MP21 4718 OX=2219138 PE=3 SV=1
MM1 pKa = 7.51LEE3 pKa = 4.1SLEE6 pKa = 4.47HH7 pKa = 6.55EE8 pKa = 4.17EE9 pKa = 4.87SSFITLTYY17 pKa = 10.06KK18 pKa = 10.86EE19 pKa = 4.57DD20 pKa = 3.65MLPFMVDD27 pKa = 3.44LPVPTLCVRR36 pKa = 11.84DD37 pKa = 3.42AQLFLKK43 pKa = 10.35RR44 pKa = 11.84LRR46 pKa = 11.84KK47 pKa = 9.99GLTDD51 pKa = 3.56HH52 pKa = 6.8ARR54 pKa = 11.84KK55 pKa = 9.53EE56 pKa = 4.36IKK58 pKa = 10.33KK59 pKa = 8.66KK60 pKa = 8.81TGKK63 pKa = 10.19KK64 pKa = 9.56KK65 pKa = 10.72LKK67 pKa = 10.4LPSFQFRR74 pKa = 11.84YY75 pKa = 9.13YY76 pKa = 10.94LCGEE80 pKa = 3.87YY81 pKa = 10.46GPRR84 pKa = 11.84GGRR87 pKa = 11.84PHH89 pKa = 5.63YY90 pKa = 10.04HH91 pKa = 7.02AILFGVPPDD100 pKa = 3.3AAKK103 pKa = 10.48FIEE106 pKa = 4.53KK107 pKa = 9.76AWSIEE112 pKa = 3.67GEE114 pKa = 4.16PIGRR118 pKa = 11.84IDD120 pKa = 3.48VQPINEE126 pKa = 4.0NNIQYY131 pKa = 9.85VAGYY135 pKa = 6.78VTKK138 pKa = 10.89KK139 pKa = 5.31MTRR142 pKa = 11.84RR143 pKa = 11.84GDD145 pKa = 3.15GRR147 pKa = 11.84KK148 pKa = 9.36PEE150 pKa = 3.98FSIMSRR156 pKa = 11.84KK157 pKa = 9.41PGIGANAVPKK167 pKa = 10.45LVEE170 pKa = 4.46LFSNPDD176 pKa = 2.86TGQYY180 pKa = 10.49FDD182 pKa = 4.66IVHH185 pKa = 6.47EE186 pKa = 4.74APVLMHH192 pKa = 6.61GRR194 pKa = 11.84KK195 pKa = 8.84QLPLGRR201 pKa = 11.84YY202 pKa = 9.1LRR204 pKa = 11.84DD205 pKa = 3.39KK206 pKa = 11.26VLDD209 pKa = 3.76EE210 pKa = 4.62MGLEE214 pKa = 4.23RR215 pKa = 11.84DD216 pKa = 3.39LTPYY220 pKa = 10.01IEE222 pKa = 5.43EE223 pKa = 4.13MFQKK227 pKa = 10.6YY228 pKa = 9.91LEE230 pKa = 4.32AKK232 pKa = 10.3RR233 pKa = 11.84SNKK236 pKa = 9.37YY237 pKa = 8.83VNCYY241 pKa = 9.85LEE243 pKa = 4.68RR244 pKa = 11.84LLIDD248 pKa = 3.42EE249 pKa = 4.42SKK251 pKa = 10.7QRR253 pKa = 11.84NLQMRR258 pKa = 11.84AIHH261 pKa = 6.72KK262 pKa = 9.93IYY264 pKa = 10.53NSRR267 pKa = 11.84NKK269 pKa = 10.55AII271 pKa = 4.02

Molecular weight: 31.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1064

271

500

354.7

39.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.613 ± 1.387

0.376 ± 0.274

5.451 ± 0.303

6.109 ± 0.674

3.853 ± 0.568

8.835 ± 1.042

2.162 ± 0.303

5.545 ± 0.673

4.511 ± 1.893

8.271 ± 0.941

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.32

3.853 ± 0.22

5.451 ± 0.418

5.451 ± 0.94

6.767 ± 0.366

6.955 ± 1.395

4.887 ± 0.908

5.357 ± 0.382

1.222 ± 0.324

4.605 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski