Gordonia phage CloverMinnie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sourvirus; unclassified Sourvirus

Average proteome isoelectric point is 5.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TKD1|A0A5J6TKD1_9CAUD Uncharacterized protein OS=Gordonia phage CloverMinnie OX=2599842 GN=83 PE=4 SV=1
MM1 pKa = 7.38ATPTFEE7 pKa = 4.0PTTLRR12 pKa = 11.84GHH14 pKa = 6.5ALLRR18 pKa = 11.84HH19 pKa = 4.79VLEE22 pKa = 5.39IIEE25 pKa = 4.25AEE27 pKa = 4.05ARR29 pKa = 11.84GDD31 pKa = 3.74GYY33 pKa = 11.72GDD35 pKa = 3.2IDD37 pKa = 4.02QISKK41 pKa = 10.49AGHH44 pKa = 6.35WDD46 pKa = 2.95QGTWGEE52 pKa = 3.93IDD54 pKa = 3.47AEE56 pKa = 4.15EE57 pKa = 4.89LIEE60 pKa = 5.37AGVDD64 pKa = 4.0FAKK67 pKa = 10.78LAVEE71 pKa = 4.52AGGEE75 pKa = 4.07SGRR78 pKa = 11.84AVDD81 pKa = 4.5LTLPTTPRR89 pKa = 11.84NLCGTACCFAGHH101 pKa = 6.45TALQVGDD108 pKa = 3.82RR109 pKa = 11.84PVVNVVVDD117 pKa = 3.45QALRR121 pKa = 11.84NDD123 pKa = 4.25LGSLTIMDD131 pKa = 4.49VRR133 pKa = 11.84PVDD136 pKa = 3.78NPDD139 pKa = 3.03VTVSVSTRR147 pKa = 11.84AMDD150 pKa = 4.3LLGLGHH156 pKa = 6.8EE157 pKa = 4.34EE158 pKa = 3.94ADD160 pKa = 4.12VLFEE164 pKa = 5.23ANNTLADD171 pKa = 4.02LRR173 pKa = 11.84VMVDD177 pKa = 3.52WICSGRR183 pKa = 11.84TVDD186 pKa = 3.9EE187 pKa = 5.21CPACGQRR194 pKa = 11.84PWDD197 pKa = 3.92CDD199 pKa = 3.37QSGEE203 pKa = 4.17ACEE206 pKa = 4.69DD207 pKa = 3.99CNEE210 pKa = 4.38HH211 pKa = 7.84LDD213 pKa = 4.74DD214 pKa = 5.67CEE216 pKa = 4.3CCADD220 pKa = 4.04CGAEE224 pKa = 3.94TGEE227 pKa = 4.32GCTCFTCMWCSSSTEE242 pKa = 4.29EE243 pKa = 5.45IDD245 pKa = 5.92GSDD248 pKa = 3.67CTCRR252 pKa = 11.84DD253 pKa = 3.95DD254 pKa = 5.79DD255 pKa = 5.99DD256 pKa = 4.1EE257 pKa = 6.93AEE259 pKa = 4.23GG260 pKa = 4.05

Molecular weight: 27.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TJY5|A0A5J6TJY5_9CAUD Uncharacterized protein OS=Gordonia phage CloverMinnie OX=2599842 GN=14 PE=4 SV=1
MM1 pKa = 7.57NKK3 pKa = 9.9RR4 pKa = 11.84LVYY7 pKa = 10.72VIGQPGSGKK16 pKa = 6.7TTLMSALTSASVRR29 pKa = 11.84LPQDD33 pKa = 3.0KK34 pKa = 9.8PVPRR38 pKa = 11.84DD39 pKa = 3.28LLATRR44 pKa = 11.84NTGRR48 pKa = 11.84VWAVEE53 pKa = 4.15LGRR56 pKa = 11.84QRR58 pKa = 11.84PGGFSGTDD66 pKa = 3.2ALASTAINTAEE77 pKa = 3.85PWLRR81 pKa = 11.84SQTEE85 pKa = 4.09APVVLAEE92 pKa = 4.18GARR95 pKa = 11.84LANRR99 pKa = 11.84RR100 pKa = 11.84FLTAAVEE107 pKa = 4.02SGYY110 pKa = 10.69QVLLVLLDD118 pKa = 3.73HH119 pKa = 7.45ADD121 pKa = 3.43AEE123 pKa = 4.52RR124 pKa = 11.84WRR126 pKa = 11.84EE127 pKa = 3.78ARR129 pKa = 11.84SVEE132 pKa = 4.22IGKK135 pKa = 8.63HH136 pKa = 5.13QNASWVKK143 pKa = 8.58GRR145 pKa = 11.84RR146 pKa = 11.84SASLNLAADD155 pKa = 4.13PPAGVEE161 pKa = 4.17VLRR164 pKa = 11.84GHH166 pKa = 7.18PSAVQEE172 pKa = 4.97PIWAWVRR179 pKa = 11.84ASS181 pKa = 3.14

Molecular weight: 19.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

19696

55

1954

234.5

25.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.982 ± 0.277

0.99 ± 0.169

7.265 ± 0.296

6.159 ± 0.375

2.417 ± 0.169

8.398 ± 0.367

1.828 ± 0.154

4.509 ± 0.289

2.701 ± 0.224

8.22 ± 0.162

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.121

2.777 ± 0.23

5.56 ± 0.238

3.437 ± 0.176

6.676 ± 0.39

5.788 ± 0.227

6.656 ± 0.195

7.773 ± 0.153

2.117 ± 0.122

2.452 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski