Klebsiella phage ST101-KPC2phi6.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J3E0|A0A482J3E0_9CAUD Uncharacterized protein OS=Klebsiella phage ST101-KPC2phi6.1 OX=2555909 PE=4 SV=1
MM1 pKa = 8.07ADD3 pKa = 4.79PITAADD9 pKa = 3.57VQAFLGEE16 pKa = 4.34LGYY19 pKa = 10.55SIPAALLDD27 pKa = 5.02PILCVVNKK35 pKa = 10.3IIPCLDD41 pKa = 2.89GAGYY45 pKa = 10.51DD46 pKa = 3.61DD47 pKa = 4.7CTAKK51 pKa = 10.83LILMYY56 pKa = 10.52AAALMATSSGARR68 pKa = 11.84RR69 pKa = 11.84IKK71 pKa = 10.8SQGTPSGASRR81 pKa = 11.84SFDD84 pKa = 3.58YY85 pKa = 11.32GDD87 pKa = 5.93DD88 pKa = 4.56GITWLRR94 pKa = 11.84DD95 pKa = 3.31SLAKK99 pKa = 10.37LDD101 pKa = 3.87TSGCTGEE108 pKa = 4.82LPISAGNSVGLFMVVGGCC126 pKa = 4.44

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J4I2|A0A482J4I2_9CAUD Recombination protein OS=Klebsiella phage ST101-KPC2phi6.1 OX=2555909 PE=4 SV=1
MM1 pKa = 7.27SNVVRR6 pKa = 11.84KK7 pKa = 10.0AGRR10 pKa = 11.84IGSGGRR16 pKa = 11.84KK17 pKa = 9.34ADD19 pKa = 3.42VSSSIQAVILVVVTNVLVTGAEE41 pKa = 4.27LKK43 pKa = 9.76RR44 pKa = 11.84TILIVAKK51 pKa = 10.27KK52 pKa = 10.07NVAGGQKK59 pKa = 10.04EE60 pKa = 4.06LLKK63 pKa = 10.51PISSVQLRR71 pKa = 11.84GIEE74 pKa = 4.42LIISSGVRR82 pKa = 11.84QALYY86 pKa = 9.49TKK88 pKa = 9.2TVSIEE93 pKa = 3.71FTGASNFFVKK103 pKa = 10.43LRR105 pKa = 11.84ACKK108 pKa = 10.5YY109 pKa = 9.64RR110 pKa = 11.84ITDD113 pKa = 2.68IGTWYY118 pKa = 10.54RR119 pKa = 11.84NLRR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 11.84WEE126 pKa = 4.0WNFCHH131 pKa = 6.67CACRR135 pKa = 11.84PP136 pKa = 3.41

Molecular weight: 15.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14526

39

856

193.7

21.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.934 ± 0.528

1.184 ± 0.142

6.079 ± 0.269

6.595 ± 0.363

3.435 ± 0.231

7.27 ± 0.317

1.838 ± 0.172

5.762 ± 0.225

5.776 ± 0.301

7.958 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.134

4.323 ± 0.266

3.717 ± 0.283

4.241 ± 0.362

6.285 ± 0.359

6.223 ± 0.266

5.741 ± 0.293

6.395 ± 0.259

1.728 ± 0.181

3.098 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski