Pseudocercospora musae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Pseudocercospora

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11907 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139II20|A0A139II20_9PEZI Protein kinase domain-containing protein OS=Pseudocercospora musae OX=113226 GN=AC579_3997 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.26EE4 pKa = 5.03NIYY7 pKa = 11.09DD8 pKa = 4.14EE9 pKa = 5.76IEE11 pKa = 4.47IEE13 pKa = 5.64DD14 pKa = 3.67MTFDD18 pKa = 3.51EE19 pKa = 4.92TTQLYY24 pKa = 9.92HH25 pKa = 6.06YY26 pKa = 7.55PCPCGDD32 pKa = 3.16RR33 pKa = 11.84FEE35 pKa = 5.85INIDD39 pKa = 3.55DD40 pKa = 3.93LRR42 pKa = 11.84GGEE45 pKa = 4.46EE46 pKa = 3.75IAVCPSCSLQIRR58 pKa = 11.84VIFDD62 pKa = 3.25VDD64 pKa = 4.13DD65 pKa = 4.14LPKK68 pKa = 10.35PKK70 pKa = 10.17EE71 pKa = 4.28PEE73 pKa = 3.94PPIPPQSAQQSLVAAAA89 pKa = 4.54

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139IC79|A0A139IC79_9PEZI Uncharacterized protein OS=Pseudocercospora musae OX=113226 GN=AC579_572 PE=4 SV=1
MM1 pKa = 7.38FDD3 pKa = 2.98ARR5 pKa = 11.84KK6 pKa = 9.34RR7 pKa = 11.84ALEE10 pKa = 3.82TAGIFGDD17 pKa = 4.08TVHH20 pKa = 6.33VSWRR24 pKa = 11.84KK25 pKa = 8.67LVSVRR30 pKa = 11.84GFCVVVPHH38 pKa = 5.69EE39 pKa = 4.05QRR41 pKa = 11.84MEE43 pKa = 4.08SGAGGNATEE52 pKa = 4.62RR53 pKa = 11.84DD54 pKa = 3.74SSRR57 pKa = 11.84SQPMPSEE64 pKa = 4.38GKK66 pKa = 9.56RR67 pKa = 11.84RR68 pKa = 11.84AAGRR72 pKa = 11.84PIMHH76 pKa = 7.49PNHH79 pKa = 7.02PSCIVRR85 pKa = 11.84RR86 pKa = 11.84AQLGRR91 pKa = 11.84TDD93 pKa = 3.23TRR95 pKa = 11.84TNLYY99 pKa = 10.59SCTTADD105 pKa = 3.15ARR107 pKa = 11.84RR108 pKa = 11.84SYY110 pKa = 8.35TQPLTPSCLTTILAGLVCTSSAQPPPPNAPKK141 pKa = 10.04WRR143 pKa = 11.84TEE145 pKa = 4.23GPNSMAIAVQQQLLYY160 pKa = 10.64KK161 pKa = 10.34FEE163 pKa = 4.62SKK165 pKa = 10.6QLTRR169 pKa = 11.84KK170 pKa = 8.33STIDD174 pKa = 3.63LPRR177 pKa = 11.84HH178 pKa = 4.75STPCQTEE185 pKa = 3.87MARR188 pKa = 11.84VEE190 pKa = 4.24TRR192 pKa = 11.84LPRR195 pKa = 11.84ASSDD199 pKa = 3.07LHH201 pKa = 6.13AVSWSASGSMSTRR214 pKa = 11.84VRR216 pKa = 11.84QAWFLVTQQ224 pKa = 4.3

Molecular weight: 24.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10380

1527

11907

5423413

25

9027

455.5

50.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.073 ± 0.021

1.322 ± 0.008

5.685 ± 0.015

6.107 ± 0.024

3.557 ± 0.015

6.784 ± 0.02

2.596 ± 0.01

4.717 ± 0.015

4.926 ± 0.023

8.565 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.009

3.661 ± 0.012

5.995 ± 0.025

4.282 ± 0.017

6.233 ± 0.02

8.202 ± 0.031

5.986 ± 0.018

5.849 ± 0.017

1.462 ± 0.009

2.744 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski