Fibrobacter sp. UWB8

Taxonomy: cellular organisms; Bacteria; FCB group; Fibrobacteres; Fibrobacteria; Fibrobacterales; Fibrobacteraceae; Fibrobacter; unclassified Fibrobacter

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2821 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5K772|A0A1M5K772_9BACT Eco47II restriction endonuclease OS=Fibrobacter sp. UWB8 OX=1896207 GN=SAMN05720760_11228 PE=4 SV=1
MM1 pKa = 7.3FKK3 pKa = 10.59KK4 pKa = 10.13IALAAALTATASFATWDD21 pKa = 3.62YY22 pKa = 11.27FPVQEE27 pKa = 4.08AHH29 pKa = 6.97KK30 pKa = 9.39GQAEE34 pKa = 3.92IHH36 pKa = 6.55FDD38 pKa = 4.49YY39 pKa = 10.75MMQDD43 pKa = 2.88DD44 pKa = 4.58WSRR47 pKa = 11.84GALSAGARR55 pKa = 11.84YY56 pKa = 8.25TIVQNFEE63 pKa = 3.87AGLIVPFVLFTSFDD77 pKa = 4.01GEE79 pKa = 4.3DD80 pKa = 3.39LKK82 pKa = 11.28QDD84 pKa = 3.81GLSDD88 pKa = 3.72LQLLLRR94 pKa = 11.84YY95 pKa = 9.96QFMPIMNAFLDD106 pKa = 4.07VSLPTCNEE114 pKa = 4.08DD115 pKa = 3.7LCGEE119 pKa = 4.52DD120 pKa = 4.22GPIGFHH126 pKa = 7.14FGVQYY131 pKa = 9.55SQKK134 pKa = 10.6FGMVDD139 pKa = 4.19FGSEE143 pKa = 3.68LGLALEE149 pKa = 4.73TKK151 pKa = 10.64GDD153 pKa = 4.15DD154 pKa = 3.58KK155 pKa = 10.31TTPPWDD161 pKa = 3.72LNLGLEE167 pKa = 4.11ADD169 pKa = 4.18FAVSEE174 pKa = 4.53MITPYY179 pKa = 10.81VGIDD183 pKa = 2.77INMLLGKK190 pKa = 8.85VTVDD194 pKa = 3.12GDD196 pKa = 4.05NVGEE200 pKa = 4.24SHH202 pKa = 6.32TGDD205 pKa = 3.26MGFAPYY211 pKa = 10.44LGLTFNINPMFYY223 pKa = 10.49AGAQAQFCFGDD234 pKa = 3.64DD235 pKa = 3.93YY236 pKa = 11.67YY237 pKa = 11.64GEE239 pKa = 4.12EE240 pKa = 4.54TITILTAKK248 pKa = 10.25VGVNFF253 pKa = 4.47

Molecular weight: 27.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5JIC0|A0A1M5JIC0_9BACT Uncharacterized protein OS=Fibrobacter sp. UWB8 OX=1896207 GN=SAMN05720760_11039 PE=4 SV=1
MM1 pKa = 7.46ASKK4 pKa = 10.84RR5 pKa = 11.84MIEE8 pKa = 3.95KK9 pKa = 10.41CKK11 pKa = 9.75RR12 pKa = 11.84TPKK15 pKa = 9.04YY16 pKa = 8.5TVRR19 pKa = 11.84GYY21 pKa = 10.37NRR23 pKa = 11.84CKK25 pKa = 10.15RR26 pKa = 11.84CGRR29 pKa = 11.84PHH31 pKa = 7.62AFMRR35 pKa = 11.84RR36 pKa = 11.84FGLCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.78MALAGEE52 pKa = 4.53IPGITKK58 pKa = 10.48SSWW61 pKa = 2.78

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2821

0

2821

1063467

30

3944

377.0

41.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.471 ± 0.055

1.34 ± 0.022

6.022 ± 0.04

6.536 ± 0.047

4.624 ± 0.038

7.255 ± 0.046

1.756 ± 0.019

5.907 ± 0.037

6.732 ± 0.041

8.699 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.02

4.617 ± 0.039

3.889 ± 0.029

2.931 ± 0.025

4.437 ± 0.039

6.697 ± 0.054

5.35 ± 0.043

7.022 ± 0.041

1.307 ± 0.018

3.802 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski