Mycobacterium phage Phasih

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GH81|A0A2D1GH81_9CAUD Holin OS=Mycobacterium phage Phasih OX=2041544 GN=SEA_PHASIH_33 PE=4 SV=1
MM1 pKa = 7.01TAATDD6 pKa = 3.37RR7 pKa = 11.84YY8 pKa = 8.53EE9 pKa = 4.74AVMCGGCEE17 pKa = 4.02VKK19 pKa = 10.61SDD21 pKa = 3.54DD22 pKa = 4.54VVYY25 pKa = 10.54GMCTACGSVEE35 pKa = 4.12VALMQPTGSYY45 pKa = 7.63PTGHH49 pKa = 6.34GCEE52 pKa = 4.09MCNN55 pKa = 4.11

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GHP7|A0A2D1GHP7_9CAUD Uncharacterized protein OS=Mycobacterium phage Phasih OX=2041544 GN=SEA_PHASIH_34 PE=4 SV=1
MM1 pKa = 8.05PDD3 pKa = 3.22RR4 pKa = 11.84YY5 pKa = 10.68GDD7 pKa = 3.63PTPEE11 pKa = 3.56PRR13 pKa = 11.84VFVRR17 pKa = 11.84PKK19 pKa = 10.42VNALTVRR26 pKa = 11.84CSWCKK31 pKa = 9.95AGVGSRR37 pKa = 11.84CVVAGTSVVLRR48 pKa = 11.84RR49 pKa = 11.84SSFHH53 pKa = 6.57DD54 pKa = 3.25VRR56 pKa = 11.84VRR58 pKa = 11.84DD59 pKa = 3.96AEE61 pKa = 4.18LAATGALARR70 pKa = 11.84GRR72 pKa = 11.84MSS74 pKa = 3.0

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

17839

30

1161

178.4

19.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.27 ± 0.443

1.34 ± 0.179

6.604 ± 0.248

5.959 ± 0.325

2.853 ± 0.196

8.739 ± 0.555

2.354 ± 0.22

4.367 ± 0.186

3.47 ± 0.231

7.265 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.141

3.453 ± 0.18

6.116 ± 0.213

3.425 ± 0.188

6.917 ± 0.406

5.785 ± 0.276

6.665 ± 0.237

7.349 ± 0.268

2.248 ± 0.141

2.59 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski