Pseudomonas phage phi15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Troedvirus; Pseudomonas virus Phi15

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0V6Z9|F0V6Z9_9CAUD Virion structural protein OS=Pseudomonas phage phi15 OX=988656 PE=4 SV=1
MM1 pKa = 7.09TLDD4 pKa = 3.41VGASKK9 pKa = 11.08ALTVAEE15 pKa = 4.25GVEE18 pKa = 4.78VVWSSSDD25 pKa = 3.61DD26 pKa = 3.65LVATVTDD33 pKa = 4.17GLVSAVSSGKK43 pKa = 10.65ANVTATYY50 pKa = 11.08GDD52 pKa = 3.8LTATCVVTVRR62 pKa = 4.17

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0V710|F0V710_9CAUD Terminase large subunit OS=Pseudomonas phage phi15 OX=988656 PE=3 SV=1
MM1 pKa = 7.5FKK3 pKa = 10.66QNSTKK8 pKa = 10.6RR9 pKa = 11.84PVTGLKK15 pKa = 9.43IRR17 pKa = 11.84RR18 pKa = 11.84GLRR21 pKa = 11.84ALPVALLIALLSLAYY36 pKa = 10.09GCASKK41 pKa = 9.92PQTPPEE47 pKa = 4.4PKK49 pKa = 10.06SKK51 pKa = 9.42VTIEE55 pKa = 3.73ASLMVKK61 pKa = 10.37PNYY64 pKa = 8.05TEE66 pKa = 4.72RR67 pKa = 11.84LLKK70 pKa = 10.6LLSEE74 pKa = 4.62

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12311

32

1326

246.2

27.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.829 ± 0.517

0.967 ± 0.147

6.222 ± 0.247

6.921 ± 0.41

3.704 ± 0.164

8.237 ± 0.337

2.071 ± 0.205

4.679 ± 0.164

5.816 ± 0.429

8.237 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.205

4.045 ± 0.272

3.931 ± 0.156

4.208 ± 0.354

5.922 ± 0.231

5.524 ± 0.291

5.613 ± 0.325

6.961 ± 0.325

1.462 ± 0.145

2.9 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski