Pseudomonas cremoricolorata

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae;

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4020 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A089WR78|A0A089WR78_9PSED DNA polymerase III subunit chi OS=Pseudomonas cremoricolorata OX=157783 GN=LK03_10250 PE=4 SV=1
MM1 pKa = 6.73QRR3 pKa = 11.84IYY5 pKa = 10.78EE6 pKa = 4.28PEE8 pKa = 3.87NLMEE12 pKa = 4.3AQMLVGMLANEE23 pKa = 4.74GIEE26 pKa = 4.04VFLIGRR32 pKa = 11.84DD33 pKa = 3.53LVGAAGEE40 pKa = 4.29LPIQGLLGLAVADD53 pKa = 4.25EE54 pKa = 4.19QAGYY58 pKa = 10.82ARR60 pKa = 11.84QLIDD64 pKa = 4.61AYY66 pKa = 11.08NAAQPVLGDD75 pKa = 4.3EE76 pKa = 4.46PDD78 pKa = 3.84SYY80 pKa = 11.23PGTLICC86 pKa = 6.31

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A089WPX1|A0A089WPX1_9PSED 30S ribosomal protein S17 OS=Pseudomonas cremoricolorata OX=157783 GN=rpsQ PE=3 SV=1
MM1 pKa = 7.38HH2 pKa = 7.55FGRR5 pKa = 11.84RR6 pKa = 11.84LQAFRR11 pKa = 11.84LWFNPKK17 pKa = 8.39RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84WAGLTLIAMAVAAMFLNPGSRR41 pKa = 11.84WGLLLGAGIYY51 pKa = 9.65WFVTSLPPFIGGRR64 pKa = 11.84RR65 pKa = 3.48

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4020

0

4020

1324866

29

7790

329.6

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.477 ± 0.041

0.992 ± 0.014

5.424 ± 0.03

5.586 ± 0.035

3.479 ± 0.023

7.999 ± 0.038

2.377 ± 0.022

4.464 ± 0.036

3.111 ± 0.037

11.982 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.205 ± 0.02

2.798 ± 0.022

4.869 ± 0.031

4.926 ± 0.034

6.771 ± 0.042

5.737 ± 0.031

4.708 ± 0.036

7.168 ± 0.038

1.439 ± 0.019

2.487 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski